Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (<i>Triticum aestivum</i>L.)

Malik, Waqar Afzal; Mahmood, Imran; Razzaq, Abdul; Afzal, M.; Shah, Ghulam Abbas; Iqbal, Asif; Zain, Muhammad; Ditta, Allah; Asad, Saeed Ahmad; Ahmad, Ishfaq; Mangi, Naimatullah; Ye, Wuwei

doi:10.1080/21645698.2021.1917975

Cited by 25 publications

(16 citation statements)

References 63 publications

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“…The response of the related parameters to AgNO3 concentration assumed a more or less concentration-dependent bell-shaped curve peaked at 4 mg L −1 . With a genotype-dependent peak, the same trend was recorded for shoot regeneration in response to the increasing concentrations of AgNO3 in the potato cultivar 'Kufri Chipsona 1′ [25,26,39], tomato [40], primrose [41], oat [42], and wheat [43]. It was also observed dealing with shoot growth and chlorophyll content in potato [26] and robusta coffee [44].…”

Section: Discussionsupporting

confidence: 59%

“…Moreover, the positive effect of AgNO 3 on in vitro growth of potato shoots was recorded by Turhan [46]. The AgNO 3 -related improvements in shoot regeneration, recorded at low concentrations, can be explained by interference with ethylene perception that ameliorates the inhibitory impact of the accumulation of the gas hormone on shoot regeneration [21,43] and shoot growth [47]. The current results reflected only 33% improvement in shoot regeneration % at 4 mg L −1 , while the average number of regenerated shoots per explant reached 2.5 folds of control.…”

Section: Discussionmentioning

confidence: 98%

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Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

et al. 2022

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Advancements in shoot regeneration systems support biotechnology-based tools used in the genetic improvement of plant crops. This study aims to enhance shoot regeneration in potatoes by boosting polyamine content by adding AgNO3 to the shoot regeneration medium (MS medium supplemented with 30 g L−1 sucrose, 100 mg L−1 myoinositol, and 2.25 BA mg L−1). Five concentrations of AgNO3 (2, 4, 6, 8, and 10 mg L−1) were used in addition to a control. The effect of AgNO3 on regeneration assumed a more or less concentration-dependent bell-shaped curve peaking at 4 mg L−1. Enhancements in shoot regeneration were attributed to the known role of AgNO3 as an ethylene action blocker in addition to improvements in polyamine accumulation without an increase in H2O2 content, lipid peroxidation, or DNA damage. The uncoupling of shoot regeneration and polyamine content recorded at high AgNO3 concentrations can be attributed to the consumption of polyamines to counteract the synchronized oxidative stress manifested by increases in H2O2 content, lipid peroxidation, and DNA damage.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 98%

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

et al. 2022

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“…Reduction in the morphogenic response observed with higher levels of the metals in the present study can be explained by the possible triggering effect they had on Fenton reaction that generates hydroxyl radicals which are known to cause lipid peroxidation, DNA and protein damage (Drazkiewicz et al 2004). In a study with AgNO 3 , CuSO 4 and their nanoparticles, Malik et al (2021) reported their differential promotory in uence on callus induction, embryogenic tissue formation and plant regeneration in Triticum aestivum genotypes in concentrationdependent manner, with the synthesized nanoparticles been more e cient over use of the two metals employed.…”

Section: Discussionmentioning

confidence: 99%

Influence of silver nitrate and copper sulphate on somatic embryogenesis, shoot morphogenesis, multiplication and associated physiological biochemical changes in Gladiolus hybridus L.

Isah

Qurratul

Umar

2022

Preprint

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Micronutrients are essential for in vitro plant tissue growth, and studying their culture medium levels manipulation to promote growth and morphogenesis is essential in plant tissue culture experiments. In the present study, influence of CuSO4.5H2O (0–15 µM), AgNO3 (0–60 µM) and their combinations on callus induction and various morphogenic processes at different treatment levels were studied in G. hybridus. AgNO3 was more efficient in promoting the parameters studied, with 40 µM been more efficient over other treatment levels tested. In the case of CuSO4.5H2O treatments, maximum efficiency was achieved with 7.5 µM over other concentration treatment of the tissues analysed. Results of the experiments on morphogenesis and associated physiological biochemical changes suggests that combined treatment with the two metals promoted morphogenesis at higher levels through influence on physiological biochemical changes essential for the morphogenic parameters studied. Application of the technique on embryogenic tissue formation and somatic embryos differentiation seems of appropriate application when using metals for the experiment.

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“…Our data contradict the aforementioned studies, but the observed discrepancy is not exceptional. Recently, Malik et al (2021) [24] reported that the calli of mature wheat embryos exposed to CuSO 4 concentrations in excess of the MS control medium showed a decrease in the frequency of somatic embryogenesis and plant regeneration. The addition of copper did not confirm the possibility of increasing regeneration in oat androgenic cultures [38] and reducing albinism in the barley microspore cultures [38].…”

Section: Discussionmentioning

confidence: 99%

“…The use of other plant growth regulators in conjunction with auxins may be useful to achieve a better balance between an efficient morphogenic response and the level of albino plant regeneration. Data from previous reports indicate that additional supplementation of cytokinin [21], an abscisic acid [22], a gibberellin inhibitor [23], or an ethylene inhibitor [24] positively affects the morphogenic response of embryo-derived cereal cultures, affecting the efficacy and the type of plant regeneration pathway.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Daminozide, Dark/Light Schedule and Copper Sulphate in Tissue Culture of Triticum timopheevii

Miroshnichenko

Klementyeva

Dolgov

2021

Plants

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Triticum timopheevii Zhuk. is a tetraploid wheat that is utilized worldwide as a valuable breeding source for wheat improvement. Gene-based biotechnologies can contribute to this field; however, T. timopheevii exhibits recalcitrance and albinism in tissue cultures, making this species of little use for manipulation through genetic engineering and genome editing. This study tested various approaches to increasing in vitro somatic embryogenesis and plant regeneration, while reducing the portion of albinos in cultures derived from immature embryos (IEs) of T. timopheevii. They included (i) adjusting the balance between 2,4-D and daminozide in callus induction medium; (ii) cultivation using various darkness/illumination schedules; and (iii) inclusion of additional concentrations of copper ions in the tissue culture medium. We achieved a 2.5-fold increase in somatic embryogenesis (up to 80%) when 50 mg L−1 daminozide was included in the callus induction medium together with 3 mg L−1 2,4-D. It was found that the dark cultivation for 20–30 days was superior in terms of achieving maximum culture efficiency; moreover, switching to light in under 2 weeks from culture initiation significantly increased the number of albino plants, suppressed somatic embryogenesis, and decreased the regeneration of green plants. Media containing higher levels of copper ions did not have a positive effect on the regeneration of green plants; contrarily, the elevated concentrations caused albinism in plantlets. The results and relevant conclusions of the present study might be valuable for establishing an improved protocol for the regeneration of green plants in tissue cultures of T. timopheevii.

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Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticum aestivumL.)

Abstract: Ye (2021): Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticumaestivum L.

Cited by 25 publications

References 63 publications

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

Influence of silver nitrate and copper sulphate on somatic embryogenesis, shoot morphogenesis, multiplication and associated physiological biochemical changes in Gladiolus hybridus L.

The Effect of Daminozide, Dark/Light Schedule and Copper Sulphate in Tissue Culture of Triticum timopheevii

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