Exploring the Antibacterial and Biosensing Applications of Peroxidase-Mimetic Ni0.1Cu0.9S Nanoflower

Abstract

Nanozymes, as artificial enzymes with the biological action of natural enzymes, have enormous potential in the fields of disease diagnosis, bacteriostasis, biosensing, etc. In this work, the Ni0.1Cu0.9S nanoflower was successfully synthesized through a one-step hydrothermal method. A combined strategy of Ni doping and morphology design was employed to adjust its electronic structure and active sites, endowing the Ni0.1Cu0.9S nanoflower with excellent peroxidase-like activity. Therefore, it can catalyze the decomposition of H2O2 to generate •OH with higher antibacterial activity, establishing a broad-spectrum antibacterial system based on the Ni0.1Cu0.9S nanoflower against E. coli and S. aureus, which avoids the harm of a high concentration of H2O2. Additionally, the colorless substrate TMB can be catalytically oxidized into blue ox-TMB via •OH. As a result, a colorimetric technique with rapid and accurate detection of ascorbic acid (AA) by the unaided eye was designed, in view of the specific inhibition effect towards the oxidation of TMB. This detection platform has a wide linear range (10~800 μM) with a low limit of detection (0.84 μM) and exhibits a satisfactory selectivity toward the detection of AA. This study sheds new light on the application of copper-containing nanozymes in the fields of biomedicine and bioassay.

Advanced nanozymes possess peroxidase-like catalytic activities in biomedical and antibacterial fields: review and progress

“…Today, the variety and quantity of nanozymes have grown exponentially, with their utilization spanning disease diagnosis and treatment, environmental protection, and sensing, among other fields. 9–11…”

“…Today, the variety and quantity of nanozymes have grown exponentially, with their utilization spanning disease diagnosis and treatment, environmental protection, and sensing, among other fields. [9][10][11] In this regard, oxidoreductase mimics have been extensively reported in the domain of antibacterial applications, which, in turn, include four enzyme-like activities: catalase (CAT)-, oxidase (OXD)-, superoxide dismutase (SOD)-, and POD-like catalytic activities. These four enzyme-simulated properties can be subdivided according to their functions and purposes.…”

Biomimetic Construction of Artificial Selenoenzymes

“…The research progress is rapid and many innovative research fields have emerged, such as selenium-containing antibody enzymes, molecularly imprinted selnoenzymes, artificial small molecular selenoenzymes, supramolecular selenoenzymes, semi-synthetic selenoenzymes, genetic engineered selenzymes, etc. The research scope of artificial selenoenzyme is expanding constantly, and its catalytic efficiency is also gradually improving [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. In this review, we summed up the progress of artificial selenoenzyme glutathione peroxidase (GPx) and several innovative strategies for constructing artificial selenoenzymes.…”