Exploring the diversity of Acinetobacter populations in river water with genus-specific primers and probes

Abstract

This study aimed to explore the diversity of river water Acinetobacter populations using culture-dependent and -independent methods. Pyrosequencing indicated that 1.5% of the total sequences from Qiandeng River water were classified as Acinetobacter. Twelve Acinetobacter strains were isolated from three different sampling sites of the Qiandeng River. Based on culture-dependent methods, A. johnsonii, A. lwoffii and A. guillouiae were the most abundantly represented Acinetobacter strains among the upper, middle and downstream populations of the river. Probing of three Acinetobacter-enriched 16S rRNA gene libraries with the Acinetobacter specific probe Act660F revealed 42 unique 16S rRNA gene sequences exhibiting a similarity of 94.9 99.9% with the known Acinetobacter strains. Among the uncultured Acinetobacter sequences, 50%, 58.3% and 68.8% of those obtained from upstream sampling site A, middle stream sampling site B and downstream sampling site C were phylogenetically located within Group I. This Group represented the most abundant strains of Acinetobacter populations in river water based on culture-independent methods. The results indicated that culture-independent methods provide more detailed information on the diversity of Acinetobacter populations than that based on culture-dependent methods. Therefore, the development of new and efficient isolation methods to identify uncultured Acinetobacter species is required.

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

“… Acinetobacter species of concern are emerging worldwide, especially in warmer climates, and their prevalence likely will increase with climate change ( 50 – 52 ). River levels of Acinetobacter species can be examined by targeted metagenomic analysis ( 53 ), amplicon assessment ( 54 ), and culture-based methods ( 55 ).…”

Differences in organic matter and bacterioplankton between sections of the largest Arctic river: Mosaic or continuum?

“…Besides, OTU 7 Acinetobacter tended to have maximum in section 3. Acinetobacter are common stream bacteria (free-living saprophytes) that have been detected in a variety of ecosystems (Leff et al 2003;Xin et al 2014) and are likely related to soil (allochthonous) organic matter (Leff et al 2003). Among the Oxalobacteraceae there are globally distributed freshwater species (Mueller-Spitz et al 2009).…”

Impacts of Flood Damage on Airborne Bacteria and Fungi in Homes after the 2013 Colorado Front Range Flood

“…This lifestyle could make these organisms better adapted to resist the biostatic agents applied to kill microorganisms in water-damaged homes, as demonstrated under laboratory conditions. 60 The abundant Acinetobacter species in flooded homes, including A. johnsonii, have been shown to form aggregates, 61 which could result in similar resistance to antimicrobial treatments, and they have been found in a variety of environments that are consistent with their abundance in indoor air from flooded homes, including river water 62 and indoor air from a turkey farm. 63 The Pantoea genus, the most abundant group of Enterobacteriaceae in flooded homes, includes organisms isolated from a wide variety of environments, including plants, water, soil, humans, and animals.…”