Exploring the influence of silver nanoparticles on the mechanism of fluorescence quenching of coumarin dye using FRET

Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials

“…The effect of dye quenching has been widely exploited for chemical and biological sensing applications, with one of the main techniques being fluorescence resonance energy transfer (FRET), in which the initially excited chromophore transfers its energy to an acceptor, thus quenching the fluorescence in the process [23,37,38]. In FRET, the donor and acceptor are molecules, and the energy transfer occurs via through space dipole-dipole interactions [39][40][41].…”

Ratiometric fluorescence assay based on carbon dots and Cu2+-catalyzed oxidation ofO-phenylenediamine for the effective detection of deferasirox

“…Moreover, according to the Benesi-Hildebrand equation (shown as formula (S1) in ESI †), the relationship between C/(DA) and 1/([OPD]) was investigated to evaluate the interaction of CDs and oxOPD. 56,57 As shown in Fig. 4C, there was a good linear relationship between C/(DA) and 1/([OPD]) (R 2 ¼ 0.9925), and the association constant (K a ) for CDs and oxOPD is 7.25 Â 10 2 M À1 , indicating the strong interaction between CDs and oxOPD.…”

“…4C, there was a good linear relationship between C/(DA) and 1/([OPD]) (R 2 ¼ 0.9925), and the association constant (K a ) for CDs and oxOPD is 7.25 Â 10 2 M À1 , indicating the strong interaction between CDs and oxOPD. 57,58 To further evaluate the degree of the combination of oxOPD and CDs, the binding constant (K b ) and the number of binding sites (n) of oxOPD with CDs were calculated by formula (S2) (shown in ESI †). 59 As shown in Fig.…”

FRET Ratiometric Nanoprobes for Nanoparticle Monitoring

“…Consequently, it has gained potential applications in many fields of science and technology lately to use metal nanoparticles in combination with fluorescent probes. Several studies have reported that metal nanoparticles of different shapes and sizes can significantly affect the fluorescence of the fluorophore within an ideal distance [ 14 , 15 ]. For instance, one study reported the application of FRET between fluorescent probe (biologically active coumarin derivative 2-acetyl-3H-benzo[ ]chromen-3-one) and microwave-assisted silver nanoparticles in propanol solvent at room temperature [ 15 ].…”

“…Several studies have reported that metal nanoparticles of different shapes and sizes can significantly affect the fluorescence of the fluorophore within an ideal distance [ 14 , 15 ]. For instance, one study reported the application of FRET between fluorescent probe (biologically active coumarin derivative 2-acetyl-3H-benzo[ ]chromen-3-one) and microwave-assisted silver nanoparticles in propanol solvent at room temperature [ 15 ]. Additionally, gold nanoparticles can also be exploited as a good energy transfer acceptor for manufacturing biosensors due to their distinctive electrodynamic properties.…”