Exploring the membrane proteome of the diazotropic cyanobacterium Anabaena PCC7120 through gel-based proteomics and in silico approaches

Sen, Sonia; Agrawal, Chitranjan; Mishra, Yogesh; Rai, Shweta; Chatterjee, Antra; Yadav, Shivam; Singh, Shilpi; Rai, L.C.

doi:10.1016/j.jprot.2015.07.022

Cited by 5 publications

(3 citation statements)

References 55 publications

(100 reference statements)

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“…Proteins are the executors of various physiological functions. Therefore, a proteomics approach is a useful technique to explore the responses of cyanobacteria at the proteome level, especially pertaining to the interaction networks among proteins (Sen et al 2015). A two-dimensional gel electrophoresis-based proteomics analysis was used to study the responses of cyanobacteria to environmental stresses, but information on hydrophobic proteins and low-abundance proteins with extreme pI values and molecular weights was usually missed (Castielli et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Stimulation effects of ciprofloxacin and sulphamethoxazole in Microcystis aeruginosa and isobaric tag for relative and absolute quantitation‐based screening of antibiotic targets

et al. 2016

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Antibiotics are normally regarded as safe to aquatic ecosystems when their contamination concentrations are lower than the toxic threshold. This study observed the hazard of ciprofloxacin, sulphamethoxazole and their binary mixture to the aquatic environment at environmentally relevant concentrations lower than the toxic threshold, due to the stimulation on the bloom of Microcystis aeruginosa. The enhanced growth of M. aeruginosa, coupled with elevated photosynthesis activity, was exerted by 50-200 ng/L of ciprofloxacin, 100-200 ng/L of sulphamethoxazole and 20-100 ng/L of the binary antibiotic mixture. Stimulated production and release of microcystins were observed at even lower concentrations. The upregulation of transcription-related proteins, cell division-related proteins, a gas vesicle protein, a global nitrogen regulator (ntcA), two microcystin synthetases (mcyC and mcyH) and ATP-binding cassette transporters provided direct proteomic evidence for the regulation of target antibiotics on M. aeruginosa bloom. Cytochrome P450 was an essential component involved in stress responses and antibiotic biodegradation. Proteomic responses to antibiotic exposure presented a shift in the energy metabolism of M. aeruginosa towards the excitation of photosynthesis, an increase of carbohydrate biosynthesis and the inhibition of carbohydrate catabolism. Superoxide dismutase, enolase and D1 protein were candidate target proteins of different antibiotics in M. aeruginosa. The antibiotic mixture showed a greater hazard than single antibiotics, and a safe threshold of 5 ng/L was suggested for each target antibiotic under the coexistence condition.

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Section: Introductionmentioning

confidence: 99%

Stimulation effects of ciprofloxacin and sulphamethoxazole in Microcystis aeruginosa and isobaric tag for relative and absolute quantitation‐based screening of antibiotic targets

et al. 2016

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“…Apart from Synechocystis , thylakoid proteomes of other cyanobacteria such as S. platensis , N. punctiforme PCC 73102, and A . PCC 7120 were also investigated. ,, Further, with the advent of nano -LC-ESI-Q-TOF MS, compositional heterogeneity in various thylakoid fractions of S . PCC 6803 by proteome analysis using nano -LC separation combined with electrospray ionization mass spectrometry was investigated and 123 proteins were identified.…”

Section: The Spatial Dynamics Of Cyanobacterial Proteomementioning

confidence: 99%

Cyanobacterial Proteomics: Diversity and Dynamics

Srivastava,

Singh,

Kanda

et al. 2024

J. Proteome Res.

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Cyanobacteria (oxygenic photoautrophs) comprise a diverse group holding significance both environmentally and for biotechnological applications. The utilization of proteomic techniques has significantly influenced investigations concerning cyanobacteria. Application of proteomics allows for large-scale analysis of protein expression and function within cyanobacterial systems. The cyanobacterial proteome exhibits tremendous functional, spatial, and temporal diversity regulated by multiple factors that continuously modify protein abundance, post-translational modifications, interactions, localization, and activity to meet the dynamic needs of these tiny blue greens. Modern mass spectrometry-based proteomics techniques enable system-wide examination of proteome complexity through global identification and high-throughput quantification of proteins. These powerful approaches have revolutionized our understanding of proteome dynamics and promise to provide novel insights into integrated cellular behavior at an unprecedented scale. In this Review, we present modern methods and cutting-edge technologies employed for unraveling the spatiotemporal diversity and dynamics of cyanobacterial proteomics with a specific focus on the methods used to analyze post-translational modifications (PTMs) and examples of dynamic changes in the cyanobacterial proteome investigated by proteomic approaches.

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“…It is noteworthy that the differentiation process can be inhibited if the nitrogen source is replenished during the time period of 9 to 12 h . By 24 h, the period of adaptation to the nitrogen deficiency, nearly all the heterocysts are mature with a full ability to fix nitrogen for the growth of cyanobacterial cells. , Although proteomic analyses have revealed the cellular mechanisms for nitrogen fixation and heterocyst differentiation in Anabaena 7120, − the previous studies on Anabaena 7120 focused mostly on the proteome changes caused by individual gene deletions or the specific proteomic changes occurring at a certain point after exposure to nitrogen stress. ,,− Additionally, these previous studies present several limitations due to the immaturity of the technology at the time they were published, such as low coverage of the proteome. Thus, it is necessary to explore the overall dynamic changes in the intracellular proteome of Anabaena 7120 in response to nitrogen stress with the proteomic technologies available nowadays.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Reveals the Protein Regulatory Network of Anabaena sp. PCC 7120 under Nitrogen Deficiency

Zhang

Wang

et al. 2021

J. Proteome Res.

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Anabaena sp. PCC 7120 (Anabaena 7120) is a photoautotrophic filamentous cyanobacterium capable of fixing atmospheric nitrogen. It is a model organism used for studying cell differentiation and nitrogen fixation. Under nitrogen deficiency, Anabaena 7120 forms specialized heterocysts capable of nitrogen fixation. However, the molecular mechanisms involved in the cyanobacterial adaptation to nitrogen deficiency are not well understood. Here, we employed a label-free quantitative proteomic strategy to systematically investigate the nitrogen deficiency response of Anabaena 7120 at different time points. In total, 363, 603, and 669 proteins showed significant changes in protein abundance under nitrogen deficiency for 3, 12, and 24 h, respectively. With mapping onto metabolic pathways, we revealed proteomic perturbation and regulation of carbon and nitrogen metabolism in response to nitrogen deficiency. Functional analysis confirmed the involvement of nitrogen stress-responsive proteins in biological processes, including nitrogen fixation, photosynthesis, energy and carbon metabolism, and heterocyst development. The expression of 10 proteins at different time points was further validated by using multiple reaction monitoring assays. In particular, many dysregulated proteins were found to be time-specific and involved in heterocyst development, providing new candidates for future functional studies in this model cyanobacterium. These results provide novel insights into the molecular mechanisms of nitrogen stress responses and heterocyst development in Anabaena 7120.

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Exploring the membrane proteome of the diazotropic cyanobacterium Anabaena PCC7120 through gel-based proteomics and in silico approaches

Cited by 5 publications

References 55 publications

Stimulation effects of ciprofloxacin and sulphamethoxazole in Microcystis aeruginosa and isobaric tag for relative and absolute quantitation‐based screening of antibiotic targets

Stimulation effects of ciprofloxacin and sulphamethoxazole in Microcystis aeruginosa and isobaric tag for relative and absolute quantitation‐based screening of antibiotic targets

Cyanobacterial Proteomics: Diversity and Dynamics

Quantitative Proteomics Reveals the Protein Regulatory Network of Anabaena sp. PCC 7120 under Nitrogen Deficiency

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