Exploring the Mesoscopic Morphology in Mussel Adhesive Proteins by Soft X-ray Spectromicroscopy

Higaki, Yasuki; Kamitani, Kazutaka; Ohigashi, Takuji; Hayakawa, Teruaki; Takahara, Atsushi

doi:10.1021/acs.biomac.0c01746

Cited by 6 publications

(4 citation statements)

References 31 publications

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“…As part of developing the analysis and evaluation, spectroscopic imaging techniques have been developed internationally in various fields, and as a pioneer, scanning transmission x-ray microscopy (STXM) applicable to the soft matter has been developed in UVSOR. To meet the recent demand for operand measurement, STXM is being further enhanced to apply to complex and heterogeneous systems [7][8][9][10][11][12][13][14][15]. In FY2020, we started the development of imaging for photoelectron spectroscopy.…”

Section: History Of Uvsormentioning

confidence: 99%

Prospects required for future light-source facilities: a case of UVSOR synchrotron facility

et al. 2023

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The synchrotron radiation facility is a large-scale public infrastructure that provides advanced light sources and is used for various academic research and application development. For 40 years, UVSOR synchrotron facility has been leading the field as a facility that has developed and utilized cutting-edge light source technology in the low-photon-energy regime. The next UVSOR synchrotron facility aims to establish a center for quantum photon science research through the development of unexplored evaluation technologies by advanced use of light sources and a measurement and analysis support environment. The science of molecular dynamics with spatio-temporal hierarchies for evaluating and controlling the functions of complex systems will be pioneered. The assets of the UVSOR synchrotron radiation facility and the fundamental measurement and analysis technology of the Institute for Molecular Science will be inherited effectively and progressively. We plan to contribute to strengthening the foundation of many fundamental disciplines to create next-generation industrial applications.

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Section: History Of Uvsormentioning

confidence: 99%

Prospects required for future light-source facilities: a case of UVSOR synchrotron facility

et al. 2023

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“…Through modelling and molecular dynamics simulations, a disordered structure and elongated conformation is found for DOPA-rich Pvfp-5 proteins and DOPA moieties are on the protein surfaces. [29,63] By extracting and analysis of these mfps from natural mussels, it is found that the diversity of catechol chemistry plays a crucial role in maintaining its adhesion behaviour. [64]…”

Section: The Structure Of Mfpsmentioning

confidence: 99%

Bioengineered Protein‐based Adhesives for Biomedical Applications

Sun

Han

Wang

et al. 2021

Chemistry A European J

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Protein-based adhesives with their robust adhesion performance and excellent biocompatibility have been extensively explored over years. In particular, the unique adhesion behaviours of mussel and sandcastle worm inspired the development of synthetic adhesives. However, the chemical synthesized adhesives often demonstrate weak underwater adhesion performance and poor biocompatibility/biodegradability, limiting their further biomedical applications. In sharp contrast, genetically engineering endows the protein-based adhesives the ability to maintain underwater adhesion property as well as biocompatibility/biodegradability. Herein, we outline recent advances in the design and development of protein-based adhesives by genetic engineering. We summarize the fabrication and adhesion performance of elastin-like polypeptide-based adhesives, followed by mussel foot protein (mfp) based adhesives and other sources protein-based adhesives, such as, spider silk spidroin and suckerin. In addition, the biomedical applications of these bioengineered protein-based adhesives are presented. Finally, we give a brief summary and perspective on the future development of bioengineered protein-based adhesives.

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“…Interface-sensitive characterizations with high spatial resolution, providing multiscale structural information about adhesive molecules at the interface, are essential to verify the above-mentioned theories. Sum frequency generation (SFG) spectroscopy − and soft X-ray absorption − have shown that functional groups of adsorbed chains are oriented at the outermost surface of the adherends. Real-space observation, such as electron microscopy, − has revealed the possibility of an interlocking or anchoring effect, , as described by the mechanical theory.…”

Section: Introductionmentioning

confidence: 99%

Electronic Interaction of Epoxy Resin with Copper at the Adhered Interface

Saeki,

Kawaguchi,

Tsuji

et al. 2024

Langmuir

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A better understanding of the aggregation states of adhesive molecules in the interfacial region with an adherend is crucial for controlling the adhesion strength and is of great inherent academic interest. The adhesion mechanism has been described through four theories: adsorption, mechanical, diffusion, and electronic. While interfacial characterization techniques have been developed to validate the aforementioned theories, that related to the electronic theory has not yet been thoroughly studied. We here directly detected the electronic interaction between a commonly used thermosetting adhesive, cured epoxy of diglycidyl ether of bisphenol A (DGEBA) and 4,4′-diaminodiphenylmethane (DDM), and copper (Cu). This study used a combination of density functional theory (DFT) calculations and femtosecond transient absorption spectroscopic (TAS) measurements as this epoxy adhesive-Cu pairing is extensively used in electronic device packaging. The DFT calculations predicted that π electrons in a DDM molecule adsorbed onto the Cu surface flowed out onto the Cu surface, resulting in a positive charge on the DDM. TAS measurements for the Cu/epoxy multilayer film, a model sample containing many metal/adhesive interfaces, revealed that the electronic states of excited DDM moieties at the Cu interface were different from those in the bulk region. These results were in good accordance with the prediction by DFT calculations. Thus, it can be concluded that TAS is applicable to characterize the electronic interaction of adhesives with metal adherends in a nondestructive manner.

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Exploring the Mesoscopic Morphology in Mussel Adhesive Proteins by Soft X-ray Spectromicroscopy

Cited by 6 publications

References 31 publications

Prospects required for future light-source facilities: a case of UVSOR synchrotron facility

Prospects required for future light-source facilities: a case of UVSOR synchrotron facility

Bioengineered Protein‐based Adhesives for Biomedical Applications

Electronic Interaction of Epoxy Resin with Copper at the Adhered Interface

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