Exploring the origin of tip‐enhanced Raman scattering; preparation of efficient TERS probes with high yield

Asghari-Khiavi, Mehdi; Wood, Bayden Robert; Hojati-Talemi, Pejman; Downes, Andrew; McNaughton, Donald; Mechler, Ádám

doi:10.1002/jrs.3021

Cited by 55 publications

(51 citation statements)

References 46 publications

(51 reference statements)

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“…One possibility is to rely upon the field enhancement induced by the gold tip. However, this enhancement is highly dependent on the local nanostructure of the tip gold coating (Asghari‐Khiavi et al ., ) especially in the case of the commercial tip used for this study.…”

Section: Resultsmentioning

confidence: 99%

Correlative infrared nanospectroscopy and transmission electron microscopy to investigate nanometric amyloid fibrils: prospects and challenges

Partouche

Mathurin

Malabirade

et al. 2019

Journal of Microscopy

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Summary Propagation of structural information through conformational changes in host‐encoded amyloid proteins is at the root of many neurodegenerative disorders. Although important breakthroughs have been made in the field, fundamental issues like the 3D‐structures of the fibrils involved in some of those disorders are still to be elucidated. To better characterise those nanometric fibrils, a broad range of techniques is currently available. Nevertheless none of them is able to perform direct chemical characterisation of single protein fibrils. In this work, we propose to investigate the structure of the C‐terminal region of a bacterial protein called Hfq as a model amyloidogenic protein, using a correlative approach. The complementary techniques used are transmission electron microscopy and a newly developed infrared nanospectroscopy technique called AFM‐IR. We introduce and discuss the strategy that we have implemented as well as the protocol, challenges and difficulties encountered during this study to characterise amyloid assemblies at the nearly single‐molecule level. Lay Description Propagation of structural information through conformational changes in amyloid proteins is at the root of many neurodegenerative disorders. Amyloids are nanostructures originating from the aggregation of multiple copies of peptide or protein monomers that eventually form fibrils. Often described as being the cause for the development of various diseases, amyloid fibrils are of major significance in the public health domain. While important breakthroughs have been made in the field, fundamental issues like the 3D‐structures of the fibrils implied in some of those disorders are still to be elucidated. To better characterise these fibrils, a broad range of techniques is currently available for the detection and visualisation of amyloid nanostructures. Nevertheless none of them is able to perform direct chemical characterisation of single protein fibrils. In this work, we propose to investigate the structure of model amyloidogenic fibrils using a correlative approach. The complementary techniques used are transmission electron microscopy and a newly developed infrared nanospectroscopy technique called AFM‐IR that allows chemical characterisation at the nanometric scale. The strategy, protocol, challenges and difficulties encountered in this approach are introduced and discussed herein.

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Section: Resultsmentioning

confidence: 99%

Correlative infrared nanospectroscopy and transmission electron microscopy to investigate nanometric amyloid fibrils: prospects and challenges

Partouche

Mathurin

Malabirade

et al. 2019

Journal of Microscopy

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“…The observed EM enhancement at the tip-apex arises from a combination of localised surface plasmon (LSP) resonance and antenna effect that result in a locally confined and enhanced electric field [24], as shown in Fig. 2a.…”

Section: Signal Enhancementmentioning

confidence: 99%

Tip-enhanced Raman spectroscopy: principles and applications

et al. 2015

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This review provides a detailed overview of the state of the art in tip-enhanced Raman spectroscopy (TERS) and focuses on its applications at the horizon including those in materials science, chemical science and biological science. The capabilities and potential of TERS are demonstrated by summarising major achievements of TERS applications in disparate fields of scientific research. Finally, an outlook has been given on future development of the technique and the mechanisms of achieving high signal enhancement and spatial resolution.

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“…[23][24][25][26] Other methods, such as galvanic reaction 27 and silver mirror reaction, 28 are not able to prepare the AFM TERS tip with a good reproducibility. [23][24][25][26] Other methods, such as galvanic reaction 27 and silver mirror reaction, 28 are not able to prepare the AFM TERS tip with a good reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Rational fabrication of a gold-coated AFM TERS tip by pulsed electrodeposition

et al. 2015

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Reproducible fabrication of sharp gold- or silver-coated tips has become the bottleneck issue in tip-enhanced Raman spectroscopy, especially for atomic force microscopy (AFM)-based TERS. Herein, we developed a novel method based on pulsed electrodeposition to coat a thin gold layer over atomic force microscopy (AFM) tips to produce plasmonic TERS tips with high reproducibility. We systematically investigated the influence of the deposition potential and step time on the surface roughness and sharpness. This method allows the rational control of the radii of gold-coated TERS tips from a few to hundreds of nanometers, which allows us to systematically study the dependence of the TERS enhancement on the radius of the gold-coated AFM tip. The maximum TERS enhancement was achieved for the tip radius in the range of 60-75 nm in the gap mode. The coated gold layer has a strong adhesion with the silicon tip surface, which is highly stable in water, showing the great potential for application in the aqueous environment.

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Exploring the origin of tip‐enhanced Raman scattering; preparation of efficient TERS probes with high yield

Cited by 55 publications

References 46 publications

Correlative infrared nanospectroscopy and transmission electron microscopy to investigate nanometric amyloid fibrils: prospects and challenges

Correlative infrared nanospectroscopy and transmission electron microscopy to investigate nanometric amyloid fibrils: prospects and challenges

Tip-enhanced Raman spectroscopy: principles and applications

Rational fabrication of a gold-coated AFM TERS tip by pulsed electrodeposition

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