Exploring the potential of benzoic acid derived from the endophytic fungus strain Neurospora crassa SSN01 as a promising antimicrobial agent in wound healing

El-Zawawy, Nessma; Ali, Sameh S.; Khalil, M. I.; Sun, Jianzhong; Nouh, Hoda S.

doi:10.1016/j.micres.2022.127108

Cited by 16 publications

(13 citation statements)

References 69 publications

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“…These data demonstrated that MRSA and MSSA did not acquire resistance to both vanillic acid and 2-hydroxycinnamic acid; by contrast, vancomycin caused the acquisition of resistance of both strains [38]. In another study, benzoic acid, purified from the endophytic fungus strain Neurospora crassa, displayed prominent antimicrobial activity against six different multidrug-resistant (MDR) clinical pathogens (see Table 1) and also confirmed its nontoxicity [39]. These findings indicate the promising aspect of phenolic acids in preventing the emergence of new resistant bacterial strains and combating with the MDR pathogens.…”

Section: Simple Phenols and Phenolic Acidsmentioning

confidence: 82%

Preventing Microbial Infections with Natural Phenolic Compounds

Ecevit

Barros

Silva

et al. 2022

Future Pharmacology

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The struggle between humans and pathogens has taken and is continuing to take countless lives every year. As the misusage of conventional antibiotics increases, the complexity associated with the resistance mechanisms of pathogens has been evolving into gradually more clever mechanisms, diminishing the effectiveness of antibiotics. Hence, there is a growing interest in discovering novel and reliable therapeutics able to struggle with the infection, circumvent the resistance and defend the natural microbiome. In this regard, nature-derived phenolic compounds are gaining considerable attention due to their potential safety and therapeutic effect. Phenolic compounds comprise numerous and widely distributed groups with different biological activities attributed mainly to their structure. Investigations have revealed that phenolic compounds from natural sources exhibit potent antimicrobial activity against various clinically relevant pathogens associated with microbial infection and sensitize multi-drug resistance strains to bactericidal or bacteriostatic antibiotics. This review outlines the current knowledge about the antimicrobial activity of phenolic compounds from various natural sources, with a particular focus on the structure-activity relationship and mechanisms of actions of each class of natural phenolic compounds, including simple phenols, phenolic acids, coumarin, flavonoids, tannins, stilbenes, lignans, quinones, and curcuminoids.

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Section: Simple Phenols and Phenolic Acidsmentioning

confidence: 82%

Preventing Microbial Infections with Natural Phenolic Compounds

Ecevit

Barros

Silva

et al. 2022

Future Pharmacology

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“…This has great value in the management of hypertrophic scarring [ 31 ]. El-Zaway et al (2022) discovered that benzoic acid could fight pathogens involved in burn wound infection, and it could be a hopeful candidate for wound healing [ 32 ]. Although no data could be found in the literature documenting a wound-healing effect for 4-hydroxybenzoic acid, the current study explores its wound-healing activity with a docking score of −4.458 kcal/mol on the active site of HMGB1 protein.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model

et al. 2023

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Excisional wounds are considered one of the most common physical injuries. This study aims to test the effect of a nanophytosomal formulation loaded with a dried hydroalcoholic extract of S. platensis on promoting excisional wound healing. The Spirulina platensis nanophytosomal formulation (SPNP) containing 100 mg PC and 50 mg CH exhibited optimum physicochemical characteristics regarding particle size (598.40 ± 9.68 nm), zeta potential (−19.8 ± 0.49 mV), entrapment efficiency (62.76 ± 1.75%), and Q6h (74.00 ± 1.90%). It was selected to prepare an HPMC gel (SPNP-gel). Through metabolomic profiling of the algal extract, thirteen compounds were identified. Molecular docking of the identified compounds on the active site of the HMGB-1 protein revealed that 12,13-DiHome had the highest docking score of −7.130 kcal/mol. SPNP-gel showed higher wound closure potential and enhanced histopathological alterations as compared to standard (MEBO® ointment) and S. platensis gel in wounded Sprague-Dawley rats. Collectively, NPS promoted the wound healing process by enhancing the autophagy process (LC3B/Beclin-1) and the NRF-2/HO-1antioxidant pathway and halting the inflammatory (TNF-, NF-κB, TlR-4 and VEGF), apoptotic processes (AIF, Caspase-3), and the downregulation of HGMB-1 protein expression. The present study’s findings suggest that the topical application of SPNP-gel possesses a potential therapeutic effect in excisional wound healing, chiefly by downregulating HGMB-1 protein expression.

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“…Sterilized cladodes were cut into 5-mm long fragments using a flame-sterilized scalpel under aseptic conditions [ 49 ]. After that, three fragments were placed separately on potato dextrose agar plates (PDA, Sigma–Aldrich, St. Louis, Missouri) supplemented with penicillin (100 U/ml) (Sigma/USA) [ 10 ]. The plates were incubated for up to 15 days at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Staphylococcus aureus and Pseudomonas aeruginosa are the most common bacteria in burn wounds [ 8 ]. Burns caused by both P. aeruginosa and S. aureus have resulted in a chronic, non-healing wound [ 9 , 10 ]. The presence of host microbiota-associated pathogens in burns could lower the efficiency of burn healing [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…They serve a crucial function in protecting the host from a variety of biotic stresses that have been shown to be associated with their antimicrobial activity [ 31 ]. Alkaloids, steroids, and amino acids have been extracted and identified as the secondary metabolites responsible for different biological activities shown by fungal endophytes [ 10 , 32 ]. Numerous studies on endophytic fungi have shown that, while some of them produce secondary metabolites that are comparable to those produced by their host plants, others produce a wide range of substances, making them a promising source of novel substances that are more bioactive than those produced by their hosts [ 33 – 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Exploring the potential of Rhizopus oryzae AUMC14899 as a novel endophytic fungus for the production of l-tyrosine and its biomedical applications

2023

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Background A significant threat to the public's health is the rise in antimicrobial resistance among numerous nosocomial bacterial infections. This may be a detriment to present initiatives to enhance the health of immune-compromised patients. Consequently, attention has been devoted to exploring new bioactive compounds in the field of drug discovery from endophytes. Therefore, this study is the first on the production of l-tyrosine (LT) as a promising bio-therapeutic agent from endophytic fungi. Results A new endophytic fungal isolate has been identified for the first time as Rhizopus oryzae AUMC14899 from Opuntia ficus-indica (L.) and submitted to GenBank under the accession number MZ025968. Separation of amino acids in the crude extract of this fungal isolate was carried out, giving a higher content of LT, which is then characterized and purified. LT exhibited strong antibacterial and anti-biofilm activities against multidrug-resistant Gram-negative and Gram-positive bacteria. The recorded minimum inhibitory concentration (MIC) values ranged from 6 to 20 µg/ml. In addition, LT caused a strong reduction in biofilm formation and disrupted the preformed biofilm. Moreover, results indicated that LT supported cell viability, evidencing hemocompatibility and no cytotoxicity. Conclusion Our findings suggest that LT has potential as a therapeutic agent due to its potential antibacterial, anti-biofilm, hemocompatibility, and lack of cytotoxic activities, which may also increase the range of therapy options for skin burn infections, leading to the development of a novel fungal-based drug.

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Exploring the potential of benzoic acid derived from the endophytic fungus strain Neurospora crassa SSN01 as a promising antimicrobial agent in wound healing

Cited by 16 publications

References 69 publications

Preventing Microbial Infections with Natural Phenolic Compounds

Preventing Microbial Infections with Natural Phenolic Compounds

Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model

Exploring the potential of Rhizopus oryzae AUMC14899 as a novel endophytic fungus for the production of l-tyrosine and its biomedical applications

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