Exploring the potential of environment friendly silver nanoparticles for DNA interaction: Physicochemical approach

Sonia, Sonia; Kukreti, Shrikant; Kaushik, Mahima

doi:10.1016/j.jphotobiol.2019.03.022

Cited by 40 publications

(13 citation statements)

References 38 publications

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“…The concentration of BP-AgNPs was made constant and CT-DNA was added gradually for the study. Figure 7 displayed a decrease in the absorption of NPs (i.e., hypochromic) with a minor bathochromic or redshift in spectra (420-424 nm) preferably indicating intercalation of BP-AgNPs with the corresponding DNA (Komal and Kaushik, 2019). The absorption spectra of CT-DNA demonstrated a minor blue shift or hypsochromic effect (264-260 nm) with the isosbestic point showing a strong interaction of NPs with CT-DNA.…”

Section: Dna Interaction Studymentioning

confidence: 99%

“…To explore the intracellular action of NPs, studies on their binding with DNA have become a primary interest in recent years (Ribeiro et al, 2018). Apart from some complicated techniques like gel electrophoresis and other electrochemical detection, UV-vis spectroscopy has been established as a convenient, accurate and extremely sensitive mode to illustrate the interaction of NPs with nucleic acids (Komal and Kaushik, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H2O2 Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken

Chandraker

Lal

Dhruve

et al. 2021

Front. Mol. Biosci.

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Bryophyllum pinnatum is a perennial herb traditionally used in ethnomedicine. In the present report, silver nanoparticles (AgNPs) were synthesized using B. pinnatum leaf extract. BP-AgNPs were confirmed following UV-Vis spectroscopy with SPR peak at 412 nm and further characterized by FTIR, XRD, SEM-EDX, and TEM. Microscopic images confirmed the spherical shape and ~15 nm average size of nanostructures. BP-AgNPs were evaluated for photocatalytic degradation of hazardous dyes (methylene blue and Rhodamine-B) and showed their complete reduction within 100 and 110 min., respectively. BP-AgNPs have emerged as a unique SPR-based novel sensor for the detection of H2O2, which may deliver exciting prospects in clinical and industrial areas. DPPH and ABTS free radical scavenging activity were studied with respective IC50 values of 89 and 259 μg/mL. A strong intercalating interaction of CT-DNA with BP-AgNPs was investigated. Observed chromosomal abnormalities confirm the antimitotic potential of BP-AgNPs in the meristematic root tip. The cytotoxicity of BP-AgNPs against B16F10 (melanoma cell line) and A431 (squamous cell carcinoma cell line), was assessed with respective IC50 values of 59.5 and 96.61 μg/ml after 24 h of treatment. The presented green synthetic approach provides a novel and new door for environmental, industrial, and biomedical applications.

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Section: Dna Interaction Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H2O2 Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken

Chandraker

Lal

Dhruve

et al. 2021

Front. Mol. Biosci.

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“…Physicochemical processes decrease the reaction time, modulating specific size, shape, crystallinity, and stability of nanoparticles. However, they need sophisticated, high-cost equipment and hazardous chemicals [177,178].…”

Section: Classification Based On the Nature Of The Deriving Forcesmentioning

confidence: 99%

Review on Natural, Incidental, Bioinspired, and Engineered Nanomaterials: History, Definitions, Classifications, Synthesis, Properties, Market, Toxicities, Risks, and Regulations

et al. 2022

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Nanomaterials are becoming important materials in several fields and industries thanks to their very reduced size and shape-related features. Scientists think that nanoparticles and nanostructured materials originated during the Big Bang process from meteorites leading to the formation of the universe and Earth. Since 1990, the term nanotechnology became very popular due to advances in imaging technologies that paved the way to specific industrial applications. Currently, nanoparticles and nanostructured materials are synthesized on a large scale and are indispensable for many industries. This fact fosters and supports research in biochemistry, biophysics, and biochemical engineering applications. Recently, nanotechnology has been combined with other sciences to fabricate new forms of nanomaterials that could be used, for instance, for diagnostic tools, drug delivery systems, energy generation/storage, environmental remediation as well as agriculture and food processing. In contrast with traditional materials, specific features can be integrated into nanoparticles, nanostructures, and nanosystems by simply modifying their scale, shape, and composition. This article first summarizes the history of nanomaterials and nanotechnology. Followed by the progress that led to improved synthesis processes to produce different nanoparticles and nanostructures characterized by specific features. The content finally presents various origins and sources of nanomaterials, synthesis strategies, their toxicity, risks, regulations, and self-aggregation.

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“…[ 1–7 ] Nanoparticles bind directly to the double helix of DNA through intercalative binding, groove binding, and electrostatic binding. [ 4,8–10 ] Intercalative mode of binding refers to nanoparticles π–π stacking interactions between adjacent base pairs of DNA resulting in structural perturbation of double helix. Nanoparticles interact with the edges of base pairs either major or minor grooves through noncovalent bonds are known as groove binding.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, coulombic interactions that occur between the negatively charged phosphate groups of DNA and the cationic portion of the nanoparticles are referred to as electrostatic binding. [ 4,8–10 ]…”

Section: Introductionmentioning

confidence: 99%

Molecular interaction studies of zinc sulphide nanoparticles with DNA and its consequence: a multitechnique approach

2020

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Molecular interaction studies between nanoparticles (NPs) and biomolecules are of great importance in the field of nanomedicine as they affect many physiological processes. Therefore, the interaction of zinc sulphide nanoparticles (ZnS NPs) with calf thymus deoxyribonucleic acid (CT DNA) and its significance was analyzed using ultraviolet (UV)–visible light, fluorescence, circular dichroism (CD), zeta potential, viscometry, electrochemical, and polymerase chain reaction methods. Fluorescence quenching analysis revealed that the fluorescence of ZnS NPs was quenched using CT DNA through a static quenching mechanism. The negative values of thermodynamic parameters (ΔG, ΔH, and ΔS) showed that the binding process was spontaneous, exothermic, and van der Waals or hydrogen bonding plays an important role in the interaction of ZnS NPs with CT DNA. Thermal melting (Tm) studies indicated a decrease in the Tm of CT DNA, suggesting the destabilization of CT DNA upon interaction with ZnS NPs. In addition, the results obtained from competitive binding, zeta potential, CD, and viscometry measurements showed that the interaction of ZnS NPs with CT DNA is through groove binding. Electrochemical analysis further confirmed the observed results from various spectroscopic and other related studies, in which decrease in the redox peak current along with changes in peak potential (CV) and increase in the electrical resistance (EIS) indicated the interaction between ZnS NPs and CT DNA. Furthermore, PCR analysis using DNA polymerase revealed the potential of ZnS NPs to inhibit DNA replication in vitro. ZnS NP–CT DNA interaction studies can be explored to define new horizons in biomedical applications of ZnS NPs.

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Exploring the potential of environment friendly silver nanoparticles for DNA interaction: Physicochemical approach

Cited by 40 publications

References 38 publications

Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H2O2 Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken

Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H2O2 Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken

Review on Natural, Incidental, Bioinspired, and Engineered Nanomaterials: History, Definitions, Classifications, Synthesis, Properties, Market, Toxicities, Risks, and Regulations

Molecular interaction studies of zinc sulphide nanoparticles with DNA and its consequence: a multitechnique approach

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