Exploring the role of E. coli derived enzyme, Oxyrase, as an oxygen scavenger to improve the cryotolerance of spermatozoa of Sahiwal bull

Ngou, Athanas Alex; Ghosh, SK; Prasad, J.K.; Katiyar, Rahul; Kumar, Abhishek; Rautela, Rupali; Bisla, Amarjeet; Srivastava, N.; Kumar, Ajay

doi:10.1016/j.cryobiol.2020.10.003

Cited by 9 publications

(13 citation statements)

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“…The present study showed that the spermatozoa in diluents con- (c) (d) 2018;Ngou et al, 2020). The optimal DO levels prevent the peroxidative damage in sperm plasma membrane, thereby strengthening the sperm plasma membrane intactness (Balamurugan et al, 2018a).…”

Section: Discussionmentioning

confidence: 60%

“…Similar findings were also reported by Kumar et al (2018) and Amin et al (2018) following reduction in the DO levels. The another reason of improved IPM at 4 ppm DO levels may be the diminished production of lipid peroxidation (LPO) products which form adduct with the sperm proteins, thus affect the motility (Ngou et al, 2020). Similarly, viability of spermatozoa was more at 4 ppm level of DO and further reduction in the level of DO was found to be detrimental for the survivability of the spermatozoa as observed at 2 ppm level of DO.…”

Section: Discussionmentioning

confidence: 99%

“…Capacitation is characterized by relocalization of phospholipids from inner to outer leaflet and leaching of cholesterol from sperm plasma membrane (Amin et al, 2018;Ngou et al, 2020). Watson (1995) speculated that these alterations in the plasma membrane make spermatozoa more vulnerable to their micro-environment at postthaw stage.…”

Section: Discussionmentioning

confidence: 99%

“…technique that has been tried by different researchers from our laboratory in the past (Amin et al, 2018;Balamurugan et al, 2018b;Bhutia, 2018;Kumar et al, 2018;Ngou et al, 2020;Pande et al, 2015). However, the precision reduction in dissolved oxygen (DO) by nitrogen gassing using trial and error method has not been done before.…”

Section: Partial Deoxygenation Of Extender Is An Innovative and Novelmentioning

confidence: 99%

“…The elevation in the ROS levels during semen cryopreservation is reported by various researchers (Balamurugan et al, 2018b;Kumar et al, 2018;Lone, Prasad, Ghosh, Das, Kumar, et al, 2016b). The levels of antioxidant components in seminal plasma decline as the antioxidant machinery associated with spermatozoa is exhausted to counterbalance the surplus free radicals during different stages of semen cryopreservation (Kumar et al, 2021;Lone et al, 2016a;2018;Ngou et al, 2020). The higher ROS production also results in premature capacitation or cryocapacitation due to cholesterol efflux and alteration in the phase behaviour of spermatozoa (Amin et al, 2018;Rajoriya et al, 2020).…”

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confidence: 96%

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Optimization of dissolved oxygen levels in extender prevents development of cryocapacitation like changes, oxidative stress and augments zona binding capacity of crossbred bull spermatozoa

et al. 2021

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Semen cryopreservation is a remarkable technique for faster and widespread propagation of superior germplasm in dairy animals.Despite the tremendous advancement in the understanding the physiology of spermatozoa during various stages of semen cryopreservation, the post-thaw recovery of viable spermatozoa still remains inadequate, especially in the crossbred bulls (Ghosh et al., 2007;Loyi, 2008;Wang et al., 1997). The average discard rate of ejaculates in the crossbred bull lies close to 55% (Mukhopadhyay et al., 2010;Tyagi et al., 2010). The poor semen freezability may be attributed to genetic, hormonal and semenological factors (Gulia et al., 2010;Sagdeo et al., 1990). Differential expression of transcripts associated with physiological processes like steroidogenesis, capacitation and acrosome reaction and cell differentiation is another aspect associated with sub-fertility in crossbred bulls (Elango

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Partial Deoxygenation Of Extender Is An Innovative and Novelmentioning

confidence: 99%

mentioning

confidence: 96%

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Optimization of dissolved oxygen levels in extender prevents development of cryocapacitation like changes, oxidative stress and augments zona binding capacity of crossbred bull spermatozoa

et al. 2021

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An evidence of Humanin-like peptide and Humanin mediated cryosurvival of spermatozoa in buffalo bulls

Katiyar

Ghosh²,

Karikalan³

et al. 2022

Theriogenology

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A review on applications and toxicities of metallic nanoparticles in mammalian semen biology

2022

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The advent of nanotechnology since the 1950s, when the well‐known physicist Richard P. Feynman talked in his famous talk about “There's plenty of room at the bottom”, has led to incredible contribution of nanotechnology in the fields of medical and veterinary therapeutics, diagnostics and other applications. Semen biology dealing with the study of spermatozoa and its related physiological and pathological aspects has not remained unscathed from the facets of nanotechnology. With each passing day investigators are revealing newer aspects of the nanoparticles, such as an antioxidants to relieve oxidative stress during semen cryopreservation, for the depletion of moribund spermatozoa from semen, gender selection of spermatozoa, bio‐imaging of gametes, sperm mediated gene transfer, as well as for male fertility evaluation. As, the uses of various magnetic nanoparticles in the industry have gained acceleration, the evaluation of their effects, either beneficial or otherwise on the mammalian spermatozoa becomes obligatory. Many toxicological studies have also been conducted in respect to the harmful effects of different metallic nanoparticles related to their applicability, and industry borne adverse effects on the male germ cells in human beings and the animals. This review has been designed to focus on the beneficial as well as toxicological effects of various metallic nanoparticles on the mammalian spermatozoa and the future prospects related to their applicability in the semen biology.

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Exploring the role of E. coli derived enzyme, Oxyrase, as an oxygen scavenger to improve the cryotolerance of spermatozoa of Sahiwal bull

Cited by 9 publications

References 42 publications

Optimization of dissolved oxygen levels in extender prevents development of cryocapacitation like changes, oxidative stress and augments zona binding capacity of crossbred bull spermatozoa

Optimization of dissolved oxygen levels in extender prevents development of cryocapacitation like changes, oxidative stress and augments zona binding capacity of crossbred bull spermatozoa

An evidence of Humanin-like peptide and Humanin mediated cryosurvival of spermatozoa in buffalo bulls

A review on applications and toxicities of metallic nanoparticles in mammalian semen biology

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