Exploring Wells-Dawson Clusters Associated With the Small Ribosomal Subunit

Abstract

The polyoxometalate P 2 W 18 , the Wells-Dawson cluster, stabilized the ribosome sufficiently for the crystallographers to solve the phase problem and improve the structural resolution. In the following we characterize the interaction of the Wells-Dawson cluster with the ribosome small subunit. There are 14 different P 2 W 18 clusters interacting with the ribosome, and the types of interactions range from one simple residue interaction to complex association of multiple sites including backbone interactions with a Wells-Dawson cluster. Although well-documented that bridging oxygen atoms are the main basic sites on other polyoxometalate interaction with most proteins reported, the W=O groups are the main sites of the Wells-Dawson cluster interacting with the ribosome. Furthermore, the peptide chain backbone on the ribosome host constitutes the main sites that associate with the Wells-Dawson cluster. In this work we investigate the potential of one representative pair of closely-located Wells-Dawson clusters being a genuine Double Wells-Dawson cluster. We found that the Double Wells-Dawson structure on the ribosome is geometrically sound and in line with other Double Wells-Dawson clusters previously observed in the solid state and solution. This information suggests that the Double Wells-Dawson structure on the ribosome is real and contribute to characterization of this particular structure of the ribosome.

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

“…Moreover, POMs have shown pharmacological activities in vitro and in vivo, such as antitumor, antimicrobial, and antidiabetic [2,3]. Their roles in biological systems are non-functional or functional kind of interactions with biomolecules [4], like the tungstate cluster that helps to solve the X-ray structure of ribosome [5] or the insulin-like properties of the decavanadates [6].…”

“…Inorganics 2020, 8, x FOR PEER REVIEW 2 of 20 the tungstate cluster that helps to solve the X-ray structure of ribosome [5] or the insulin-like properties of the decavanadates [6].…”

"Ultrastructure of the Mitochondrial Membrane and Respiratory Ensembles"

“…Tedeschi and Harris have convincingly shown that the permeability of the mitochondrial membrane also follows these patterns. Biological membranes are characterized by very high electrical resistance and capacitance; this implies the presence of structures containing a continuous dielectric layer, which can be created by a continuous lipid phase [29]. Pauli has recently established that the mitochondrial membrane is also characterized by high electrical resistance.…”

Exploiting Interactions between Polyoxometalates and Proteins for Applications in (Bio)chemistry and Medicine

“…Additionally, the favorable binding of POMs to protein surfaces renders them ideally suited for facilitating the crystallization of proteins and solving their structures through X‐ray diffraction [15–17] . The most prominent example of this is perhaps the important dual role of POMs in generating phases and inducing post‐crystallization rearrangements that allowed the structure of the ribosome to be solved, for which the 2009 Nobel Prize in Chemistry was awarded [15–18] …”