Export of polybasic motif–containing secretory proteins BMP8A and SFRP1 from the endoplasmic reticulum is regulated by surfeit locus protein 4

Tang, Xiao Song; Wang, Tingxuan; Guo, Yusong

doi:10.1016/j.jbc.2022.102687

Cited by 7 publications

(8 citation statements)

References 28 publications

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“…Recent studies have also suggested a key role for the Cardin–Weintraub (CW) motif [K/R][K/R][K/R]XX[K/R][K/R] in recognition by SURF4 for cargoes such as the hedgehog family. , Analysis of the human proteome identified 267 proteins carrying a CW motif among 7327 proteins that carry a signal peptide or a transmembrane domain. Among the 115 SURF4 candidate cargoes identified by our and previous studies (Figure C), seven carry a CW motif, though only one of these seven proteins was identified in the current study (Table S13).…”

Section: Resultsmentioning

confidence: 99%

“…Erv29p facilitates the secretion of yeast pro-α-factor, carboxypeptidase Y, and proteinase A. – Multiple SURF4 cargoes in mammals have been identified to date, including PCSK9, – apolipoprotein B (APOB) – growth hormone, dentin sialophosphoprotein (DSPP), amelogenin, erythropoietin, pathogenic SERPINA1 polymers, sonic hedgehog, proinsulin, and the lysosomal proteins progranulin and prosaposin . Two SURF4-binding motifs on cargoes have been proposed, including an ER-ESCAPE tripeptide motif immediately downstream of the signal peptide sequence and a Cardin–Weintraub motif, , though not all of the putative cargoes listed above carry one of these motifs, suggesting the presence of additional determinants for recognition by SURF4 …”

Section: Introductionmentioning

confidence: 99%

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Identification of LMAN1- and SURF4-Dependent Secretory Cargoes

Tang,

Abbineni,

Veiga Leprevost

et al. 2023

J. Proteome Res.

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Most proteins secreted into the extracellular space are first recruited from the endoplasmic reticulum into coat protein complex II (COPII)-coated vesicles or tubules that facilitate their transport to the Golgi apparatus. Although several secreted proteins have been shown to be actively recruited into COPII vesicles and tubules by the cargo receptors LMAN1 and SURF4, the full cargo repertoire of these receptors is unknown. We now report mass spectrometry analysis of conditioned media and cell lysates from HuH7 cells CRISPR targeted to inactivate the LMAN1 or SURF4 gene. We found that LMAN1 has limited clients in HuH7 cells, whereas SURF4 traffics a broad range of cargoes. Analysis of putative SURF4 cargoes suggests that cargo recognition is governed by complex mechanisms rather than interaction with a universal binding motif..

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of LMAN1- and SURF4-Dependent Secretory Cargoes

Tang,

Abbineni,

Veiga Leprevost

et al. 2023

J. Proteome Res.

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“…A subset of putative SURF4 cargoes carry the previously described ER-ESCAPE (29) or CW motifs (32,35) potentially mediating interaction with SURF4. However, most do not, suggesting a more complex mechanism governing SURF4 cargo recognition.…”

Section: Discussionmentioning

confidence: 99%

“…in recognition by SURF4 for cargoes such as the hedgehog family (32,35). Analysis of the human proteome identified 267 proteins carrying a CW motif among 7327 proteins that carry a signal peptide or a transmembrane domain.…”

Section: Most Surf4-dependent Secreted Proteins Lack An Er-escape Motifmentioning

confidence: 99%

“…Two SURF4 binding motifs on cargoes have been proposed, including an ER-ESCAPE tripeptide motif immediately downstream of the signal peptide sequence (29) and a Cardin-Weintraub motif (32,35), though not all of the putative cargoes listed above carry one of these motifs, suggesting the presence of additional determinants for recognition by SURF4 (24).…”

Section: Introductionmentioning

confidence: 99%

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Identification of LMAN1 and SURF4 dependent secretory cargoes

Tang

Abbineni

Leprevost

et al. 2023

Preprint

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Most proteins secreted into the extracellular space are first recruited from the endoplasmic reticulum into coat protein complex II (COPII)-coated vesicles or tubules that facilitate their transport to the Golgi apparatus. Although several secreted proteins have been shown to be actively recruited into COPII vesicles/tubules by the cargo receptors LMAN1 and SURF4, the full cargo repertoire of these receptors is unknown. We now report mass spectrometry analysis of conditioned media and cell lysates from HuH7 cells CRISPR targeted to inactivate the LMAN1 or SURF4 gene. We found that LMAN1 has limited clients in HuH7 cells whereas SURF4 traffics a broad range of cargoes. Analysis of putative SURF4 cargoes suggests that cargo recognition is governed by complex mechanisms rather than interaction with a universal binding motif.

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Conventional and Unconventional Protein Secretion in Yeast and Animal Cells

Tang,

Guo

2024

Methods in Molecular Biology

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Export of polybasic motif–containing secretory proteins BMP8A and SFRP1 from the endoplasmic reticulum is regulated by surfeit locus protein 4

Cited by 7 publications

References 28 publications

Identification of LMAN1- and SURF4-Dependent Secretory Cargoes

Identification of LMAN1- and SURF4-Dependent Secretory Cargoes

Identification of LMAN1 and SURF4 dependent secretory cargoes

Conventional and Unconventional Protein Secretion in Yeast and Animal Cells

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