Exposing hidden diversity by concordant genealogies and morphology—a study of the Lactifluus volemus (Russulales) species complex in Sikkim Himalaya (India)

Putte, Kobeke Van de; Nuytinck, Jorinde; Das, Kanad; Verbeken, Annemieke

doi:10.1007/s13225-012-0162-0

Cited by 34 publications

(45 citation statements)

References 43 publications

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“…Stubbe and L. subg. Russulopsis (Verbeken) Verbeken are strongly similar to Lactarius and Russula, respectively (Verbeken 2001, Stubbe et al 2010, Verbeken et al 2011. Microscopically the genus has more types of pileipellis and stipitipellis than Lactarius and Multifurca, varying from cutis to palisade, over trichoderm or trichopalisade, with or without ixo layers, with or without thick-walled elements, with or without dermatocystidia and including some deviating types.…”

Section: Introductionmentioning

confidence: 99%

“…Microscopically the genus has more types of pileipellis and stipitipellis than Lactarius and Multifurca, varying from cutis to palisade, over trichoderm or trichopalisade, with or without ixo layers, with or without thick-walled elements, with or without dermatocystidia and including some deviating types. The morphological richness is still growing with new representatives discovered from the tropics (van de Putte et al 2009, Miller et al 2012, Wang et al 2012, Morozova et al 2013. Buyck et al (2008) presented a three-locus phylogeny of Lactifluus (as "Lactarius 1").…”

Section: Introductionmentioning

confidence: 99%

“…It contained 15 species, belonging to the four subgenera proposed by Verbeken et al (2011. Before and after Buyck et al (2008) additional taxa from Africa, America and Asia were documented with molecular data, either using a single locus or a multigene approach (Henkel et al 2000;Buyck et al 2007;Stubbe et al 2010;van de Putte et al 2010van de Putte et al , 2012Wang et al 2012;Morozova et al 2013;de Crop et al 2014a;Maba et al 2014Maba et al , 2015. These taxa represented all six currently described subgenera (Verbeken et al 2011Stubbe et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Polysphaerophori (Singer) Verbeken has not been tested using molecular data. Several morphology-based subgeneric taxa have been suggested to be paraphyletic using molecular phylogenetics (Buyck et al 2008, Stubbe et al 2010, van de Putte et al 2010, but further sampling of species and genes are needed to confirm this. Molecular data are needed to clarify the relationships of species with still uncertain or isolated systematic positions (Wang and Verbeken 2006, Miller et al 2012, for example, L. subiculatus S. L Miller et al, L. cocosmus (van de Putte & de Kesel) van de Putte, L. tenuicystidiatus (X.H.…”

Section: Introductionmentioning

confidence: 99%

“…Gerardii and L. subg. Piperati Verbeken) has demonstrated high diversity in this still largely unexplored continent (Stubbe et al 2010van de Putte et al 2010van de Putte et al , 2012Wang et al 2012;Morozova et al 2013;de Crop et al 2014a). The current study revisiting Asia with sampling in southern China added three new distinct lineages to Lactifluus.…”

Section: Introductionmentioning

confidence: 99%

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Revisiting the morphology and phylogeny ofLactifluuswith three new lineages from southern China

et al. 2015

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As a recent group mainly defined by molecular data the genus Lactifluus is in need of further study to provide insight into the morphological and molecular variation within the genus, species limits and relationships. Phylogenetic analyses of nuc rDNA ITS1-5.8S-ITS2 (ITS), D1 and D2 domains of nuc 28S rDNA (28S), and part of the second largest subunit of the RNA polymerase II (rpb2) (6-7 region) sequences of 28 samples from southern China revealed three new lineages of Lactifluus. Two of them are nested in a major clade that includes the type of Lactifluus and here is treated as two new sections: L. sect. Ambicystidiati and L. sect. Tenuicystidiati. Lactifluus ambicystidiatus, described here as a new species (5 sect. Ambicystidiati), has both lamprocystidia and macrocystidia in the hymenium, a unique combination of features within Russulaceae. Furthermore, only remnants of lactiferous hyphae are present in L. ambicystidiatus and our results suggest that the ability to form a lactiferous system has been lost in this lineage. Lactifluus sect. Tenuicystidiati forms a strongly supported monophyletic group as a sister lineage to L. sect. Lactifluus. We recognize it based on the thin-walled macrocystidia and smaller ellipsoid spores with an incomplete reticulum compared with L. sect. Lactifluus. The former placement of L. tenuicystidiatus in the African L. sect. Pseudogymnocarpi is not supported. Using genealogical concordance we recognize five phylogenetic species within L. sect. Tenuicystidiati and describe two of these as new, L. subpruinosus and L. tropicosinicus. The third lineage, represented by L. leoninus, forms a sister group to L. subg. Lactariopsis sensu stricto. The three lineages provide further evidence for morphological features in Lactifluus being homoplasious. Some sections and species complexes are likely to be composed of more species and merit further investigations. Subtropical-tropical Asia is likely a key region for additional sampling.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%