Exposure of turkey sperm to a synthetic peptide before insemination increases fertility

Gill, S. P. S.; Donoghue, Ann M.; Amann, R. P.

doi:10.1093/ps/79.3.426

Cited by 4 publications

(4 citation statements)

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“…Exposure of frozen thawed bull (Amann et al, 1999b) or rooster (Gill et al, 1999) sperm increased pregnancy rate or number of chicks hatched. Exposure of fresh turkey sperm to peptide S12-Ox increased poult production (Gill et al, 2000).…”

Section: Is the Upsebp System Important?mentioning

confidence: 95%

“…We concluded that the intervening sequence between saposins A and B (Figure 1) and UPSEBP were similar and had similar biological activity; namely, increasing egg binding and fertility of rooster sperm. An abstract (Hammerstedt et al, 1997) presented early data, but 4 reports on bioactivity of the synthetic sequence (Amann et al, 1999a(Amann et al, , 1999b(Amann et al, , 1999cGill et al, 2000) bypassed the biological underpinning and path to the sequence used. This biological information is provided herein.…”

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confidence: 99%

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A Fragment of Prosaposin (SGP‐1) from Rooster Sperm Promotes Sperm‐Egg Binding and Improves Fertility in Chickens

HAMMERSTEDT,

CRAMER,

BARBATO

et al. 2001

Journal of Andrology

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A protein isolated from the supernatant of cryopreserved rooster sperm was found to increase the capability of cryopreserved rooster sperm to bind in vitro to the perivitelline membrane of a chicken egg and substantially raise fertility after artificial insemination (AI). That activity was partially purified and termed universal primary sperm‐egg binding protein (UPSEBP). Insufficient protein remained from 6 × 1011 sperm, despite retention of bioactivity, to allow sequencing. We deduced that the protein may be related to prosaposin (also termed SGP‐1, for sulfated glycoprotein‐1), and we used published amino acid sequences of prosaposin as a guide for synthesis of peptides. Certain peptides were found to increase in vitro sperm‐egg binding and increase fertility of frozen‐thawed or fresh rooster sperm, in a manner similar to semipurified UPSEBP. Active epitopes were in a 60 amino acid sequence, reflecting the intervening sequence between saposins A and B, plus short extensions into saposins A and B. Highest activity was found when this synthetic peptide was oxidized to form a disulfide bond between terminal cysteines. Antibody against a synthetic peptide consisting of 58 of these 60 amino acids bound to a 7–9 kilodalton protein in UPSEBP. Collectively, the data support the conclusion that UPSEBP is a fragment of prosaposin. Because prosaposin is in semen in humans and animals, these observations have broad implications for possible cause and therapy of one type of subfertility.

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Section: Is the Upsebp System Important?mentioning

confidence: 95%

mentioning

confidence: 99%

A Fragment of Prosaposin (SGP‐1) from Rooster Sperm Promotes Sperm‐Egg Binding and Improves Fertility in Chickens

HAMMERSTEDT,

CRAMER,

BARBATO

et al. 2001

Journal of Andrology

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“…O teste também tem sido usado para avaliar se a versão sintética (FertPlus®) de um fragmento da prosaposina, presente no plasma seminal, e envolvido na ligação dos espermatozóides à membrana perivitelina de aves, aumentaria a ligação e a fertilidade dos espermatozóides de touros , cachaços , perus (Gill et al, 1999a) e homens (Amann et al, , 1999c.…”

Section: Boar Semen Fertility Evaluated By a Sperm-binding Assay To Aunclassified

Fertilidade de sêmen suíno avaliada pelo teste de ligação dos espermatozóides a um substrato sintético

Reis

Bernardi

Wentz

et al. 2003

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a fertilidade de sêmen suíno pelo teste de ligação de espermatozóides a um substrato sintético. A motilidade (MOT) e o porcentual de espermatozóides ligados (PEL) foram avaliados após 5, 24, 48 e 72 horas de armazenamento a 17ºC. O PEL foi determinado em soluções contendo 6,25 ou 12,5 milhões de espermatozóides/mL, com ou sem albumina sérica bovina (BSA), preparadas a partir de dois a cinco ejaculados de cada um dos quatro machos. Cinqüenta e oito leitoas foram inseminadas, uma vez, 24 horas após o início do estro. Houve correlação positiva (P = 0,0001; r = 0,33) entre a MOT e o PEL. O PEL foi maior com 12,5 milhões de espermatozóides/mL e na presença de BSA (P<0,05). Após 72 horas, o macho 3 apresentou PEL inferior ao dos outros três (P<0,05). As taxas de clivagem (TC) e de embriões morfologicamente normais não diferiram entre indivíduos, mas o macho 3 apresentou menos de 70,0% de TC no quartil superior, enquanto os outros tiveram mais de 75,0%. Os machos diferem quanto à capacidade de ligação de seus espermatozóides ao substrato sintético, a partir de 24 horas de armazenamento do sêmen. A ligação dos espermatozóides ao substrato sintético é maior com a inclusão de BSA e com o aumento da concentração espermática.Termos para indexação: albumina sérica, inseminação artificial, ligação espermatozóide-ovócito. Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrateAbstract -The objective of this work was to evaluate boar semen fertility by a sperm-binding assay to a synthetic substrate. Motility (MOT) and percentage of bound sperm (PSB) were evaluated after 5, 24, 48 and 72 hours of storage at 17°C. PSB was analyzed in solutions containing 6.25 or 12.5 million of spermatozoa/mL, with or without bovine serum albumin (BSA), processed from two to five ejaculates of four boars. Fifty eight gilts were inseminated, a single time, 24 hours after the beginning of estrus. There was a positive correlation (P = 0.0001; r = 0.33) between MOT and PSB. Higher percentages of PSB were observed with 12.5 million of spermatozoa/mL and in the presence of BSA (P<0.05). After 72 hours, boar 3 showed lower PSB (P<0.05) than the other boars. The cleavage (TC) and normal embryo rates did not differ among boars, but boar 3 showed less than 70.0% of TC belonging to the superior quartile while boars 1, 2 and 4 had more than 75.0%. After 24 hours of sperm storage, boars differ in their sperm binding to the synthetic substrate. Binding of swine spermatozoa to the synthetic substrate is higher in the presence of BSA and with the increase of spermatic concentration.

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“…In addition, ef®ciency of IVF differs with mouse strain (Tada et al, 1990;Songsasen and Liebo, 1997) for unknown reasons. Recent reports (Barbato et al, 1998;Amann et al, 1999a, b, c;Gill et al, 1999Gill et al, , 2000Amann and Gill, 2000), testing a variety of avian and mammalian species describe: (a) an in vitro assay capable of distinguishing differences in sperm±ovum binding between individual males (and strains); and (b) the ability of exogenous peptide to alter fertilization rate. Barbato et al (1998) established the mouse sperm could bind to the test matrix, but provided neither an analysis of any stain-to-strain differences nor any evidence that extent of binding was related to fertilization potential.…”

Section: Introductionmentioning

confidence: 99%

Increased in vitro binding and fertilizing ability of mouse sperm exposed to a synthetic peptide

2000

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We report use of an in vitro assay (Barbato et al., 1998: Biol Reprod 58:686–699) to assess binding ability of cauda epididymal mouse sperm to a surrogate zona pellucida and effect of a synthetic peptide (Amann et al., 1999: J Androl 20: 42–46) on fertilization ability in in vitro fertilization (IVF) tests. Sperm from C57Bl/6, CD1, and CF1 mice (4 replicates each) were evaluated for binding ability after exposure to 0 (control) and 80–1280 pM peptide. For control sperm, endogenous binding was C57Bl/6 < CD1 = CF1 (P < 0.05, 1‐way ANOVA). Across all three strains, exposure to > 320 pM peptide increased relative binding of sperm (P < 0.05; 2‐way general linear model; GLM). Strains differed both in basal binding ability and in response to synthetic peptide. To determine if IVF rate increased after exposure of sperm to peptide, ova from B6C3 mice (four replicate pools) were collected after eCG and hCG stimulation. Cumulus–oocyte complexes (COC; 8–15 ova in each of 3–6 drops/treatment) were incubated with hyperactivated C57Bl/6 sperm at ∼1500 sperm per ovum. Data for incubations were corrected for false‐positive classification to yield a better estimate of true cleavage rate, and then related to results observed with a tenfold greater sperm concentration. Relative cleavage rates were 0 peptide (0.48); 420 pM (0.78, P < 0.05); and 840 pM (0.90, P < 0.01; GLM and Tukey tests). IVF rate was increased by exposure of mouse sperm to peptide at concentrations effective in the in vitro assay, and use of peptide allowed use of 1/10 as many sperm. Mol. Reprod. Dev. 57:406–411, 2000. © 2000 Wiley‐Liss, Inc.

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Exposure of turkey sperm to a synthetic peptide before insemination increases fertility

Cited by 4 publications

References 5 publications

A Fragment of Prosaposin (SGP‐1) from Rooster Sperm Promotes Sperm‐Egg Binding and Improves Fertility in Chickens

A Fragment of Prosaposin (SGP‐1) from Rooster Sperm Promotes Sperm‐Egg Binding and Improves Fertility in Chickens

Fertilidade de sêmen suíno avaliada pelo teste de ligação dos espermatozóides a um substrato sintético

Increased in vitro binding and fertilizing ability of mouse sperm exposed to a synthetic peptide

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