Exposure to cadmium during in vitro maturation at environmental nanomolar levels impairs oocyte fertilization through oxidative damage: A large animal model study

Martino, Nicola Antonio; Marzano, Giuseppina; Mangiacotti, Michele; Miedico, Oto; Sardanelli, Anna Maria; Gnoni, Antonio; Lacalandra, Giovanni Michele; Chiaravalle, Antonio Eugenio; Ciani, Elena; Bogliolo, Luisa; Minervini, Fabrizio; Pizzi, Flavia; Dell’Aquila, Maria Elena

doi:10.1016/j.reprotox.2017.02.005

Cited by 33 publications

(31 citation statements)

References 50 publications

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“…It is also a possibility that there might be more than one mechanism in operation at the same time. In addition, Cd may exert its effect by interacting with membrane transporters involved in the uptake of nutritive metals, such as calcium, iron, zinc, copper and manganese, through a process called “ionic mimicry” or alternatively by depleting cell protein sulfhydryl reserves and interfering with normal protein folding [11] or by inducing cumulus-oocyte mitochondria over-activity and oxidative damage as has been reported in case of ovine COCs [36]. However, it was not the aim of this study to investigate how Cd may achieve its effect on IVM of bovine oocytes and subsequent IVF and embryo development and therefore, is a matter for further research.…”

Section: Discussionmentioning

confidence: 99%

The effect of cadmium on the bovinein vitrooocyte maturation and early embryo development

Akar

Ahmad

Khalid

2018

International Journal of Veterinary Science and Medicine

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Common pollutants such as heavy metals and cadmium is among those with high environmental concerns. In vivo studies had shown that cadmium (Cd) causes oocyte degeneration and embryo mortality, and lowers pregnancy rates in mammals. However, there is limited information available about direct effects of Cd on oocyte maturation and/or embryo development. This study was aimed to investigate if Cd has any effect on the oocyte maturation and/or embryo development in vitro. Bovine COCs were collected from the slaughter house and cultured for 24 h in serum-free media only (Controls) or supplemented with 0.2, 2.0 and 20.0 μM CdCl2. At 24 h cumulus cell expansion was assessed in all COCs. COCs were either denuded and stained for determination of nuclear maturation or fertilized for assessment of subsequent embryo development. Cd at the lowest concentration (0.2 μM) did not affect any of the parameters studied. However, at higher concentrations (2.0 and 20.0 μM) it significantly (P < 0.05) reduced the percentage of fully-expanded COCs and significantly (P < 0.05) increased the percentage of partially and/or non-expanded COCs compared to controls and 0.2 μM. Cadmium at higher concentrations (2.0 and 20.0 μM) also significantly (P < 0.01) reduced the percentage of oocytes reaching metaphase II stage compared to controls and 0.2 μM. Post-fertilization cleavage rate in presumptive zygotes and blastocyst development significantly (P < 0.05) reduced 0.2, 2.0 and 20.0 μM CdCl2 compared to the controls (0.0 μM). In conclusion, these results suggest that Cd had direct detrimental effects on the bovine oocyte maturation and its developmental competence.

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Section: Discussionmentioning

confidence: 99%

The effect of cadmium on the bovinein vitrooocyte maturation and early embryo development

Akar

Ahmad

Khalid

2018

International Journal of Veterinary Science and Medicine

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“…Oocyte exposure (maternal effect). It has been well-documented that Cd exposure negatively affects oocyte maturation, subsequent fertilization and embryo development [ 24 ]. 2.…”

Section: Discussionmentioning

confidence: 99%

Reproductive effects of cadmium on sperm function and early embryonic development in vitro

et al. 2017

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Cadmium is a major environmental toxicant that is released into the atmosphere, water and soil in the form of cadmium oxide, cadmium chloride, or cadmium sulfide via industrial activities, such as the manufacturing of batteries and pigments, metal smelting and refining and municipal waste incineration. In the present study, we investigated the effects of cadmium exposure on sperm quality parameters, fertilization capacity and early embryonic development. Our study showed that in vitro incubation of human or mouse sperms with cadmium for a long time (up to 24 hours) could significantly decreased sperm motility in a concentration- and time-dependent manner. Exposure to cadmium in the environment for a short term (30 min) did not affect sperm motility but significantly reduced in vitro fertilization rate. We also evaluated the effects of cadmium at concentrations of 0.625 μg/ml, and 1.25 μg/ml on early embryonic development in vitro and observed that the blastocyst formation rate dramatically decreased with increasing cadmium concentration. This finding emphasizes the hazardous effects of cadmium on sperm quality as well as on natural embryo development and raises greater concerns regarding cadmium pollution.

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“…For COC retrieval, ovaries from adult cyclic ewes underwent follicular fluid aspiration using an 18 G needle followed by slicing [52]. Follicular contents were released in sterile Petri dishes containing phosphate buffered saline (PBS) supplemented with 1 mg/mL heparin.…”

Section: Coc Retrievalmentioning

confidence: 99%

One-step automated bioprinting-based method for cumulus-oocyte complex microencapsulation for 3D in vitro maturation

Mastrorocco¹,

Cacopardo²,

Martino³

et al. 2020

PLoS ONE

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Three-dimensional in vitro maturation (3D IVM) is a promising approach to improve IVM efficiency as it could prevent cumulus-oocyte complex (COC) flattening and preserve its structural and functional integrity. Methods reported to date have low reproducibility and validation studies are limited. In this study, a bioprinting based production process for generating microbeads containing a COC (COC-microbeads) was optimized and its validity tested in a large animal model (sheep). Alginate microbeads were produced and characterized for size, shape and stability under culture conditions. COC encapsulation had high efficiency and reproducibility and cumulus integrity was preserved. COC-microbeads underwent IVM, with COCs cultured in standard 2D IVM as controls. After IVM, oocytes were analyzed for nuclear chromatin configuration, bioenergetic/oxidative status and transcriptional activity of genes biomarker of mitochondrial activity (TFAM, ATP6, ATP8) and oocyte developmental competence (KHDC3, NLRP5, OOEP and TLE6). The 3D system supported oocyte nuclear maturation more efficiently than the 2D control (P<0.05). Ooplasmic mitochondrial activity and reactive oxygen species (ROS) generation ability were increased (P<0.05). Up-regulation of TFAM, ATP6 and ATP8 and down-regulation of KHDC3, NLRP5 expression were observed in 3D IVM. In conclusion, the new bioprinting method for producing COC-microbeads has high reproducibility and efficiency. Moreover, 3D IVM improves oocyte nuclear maturation and relevant parameters of oocyte cytoplasmic maturation and could be used for clinical and toxicological applications.

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Exposure to cadmium during in vitro maturation at environmental nanomolar levels impairs oocyte fertilization through oxidative damage: A large animal model study

Cited by 33 publications

References 50 publications

The effect of cadmium on the bovinein vitrooocyte maturation and early embryo development

The effect of cadmium on the bovinein vitrooocyte maturation and early embryo development

Reproductive effects of cadmium on sperm function and early embryonic development in vitro

One-step automated bioprinting-based method for cumulus-oocyte complex microencapsulation for 3D in vitro maturation

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