Exposure to the Polychlorinated Biphenyl Mixture Aroclor® 1254 Alters Melanocyte and Tail Muscle Morphology in Developing Xenopus Laevis Tadpoles

Fisher, Marla A.; Jelaso, Anna M.; Predenkiewicz, Amy; Schuster, Lindsey; Means, Jay C.; Ide, Charles F.

doi:10.1897/1551-5028(2003)022<0321:ettpbm>2.0.co;2

Cited by 7 publications

(11 citation statements)

References 34 publications

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“…For both studies, similar health effects appeared in some tadpoles exposed to 500 ppb. This is in agreement with our previous studies that quantitatively analyzed Aroclor 1254-induced changes in growth, gross morphology, and behavior in developing tadpoles [Fisher et al, 2003;Jelaso et al, 2002].…”

Section: Aroclor 1254 Alters Gross Morphology and Swimming Behaviorsupporting

confidence: 93%

“…In some cases tadpoles from different spawnings responded somewhat differently to Aroclor 1254. This difference also occurred in our previous studies and is most likely due to genetic variability among the spawnings [Fisher et al, 2003;Jelaso et al, 2002]. However, Burgin et al [2001] reported that individual lots of commercially available Aroclor 1254 produce differential effects.…”

Section: Discussionsupporting

confidence: 64%

“…Previous studies showed that Aroclor 1254 alters gross morphological development and behavior in 5-and 11-day PF tadpoles [Fisher et al, 2003;Jelaso et al, 2002]. In this report, gross morphological development and behavior were monitored in 18-day PF tadpoles.…”

Section: Chemical Exposuresmentioning

confidence: 81%

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Gene expression patterns predict exposure to PCBs in developing Xenopus laevis tadpoles

Jelaso

Lehigh-Shirey

Means

et al. 2003

Environ and Mol Mutagen

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Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants that pose global ecological and human health problems. Although it is well established that PCBs are associated with a variety of adverse health effects in wildlife and in humans, it is often difficult to determine direct cause-and-effect relationships between exposure and specific health outcomes. In this study, gene expression signatures were used to relate exposure to PCBs with altered physiological responses and/or specific health effects. Real-time PCR was used to measure gene expression levels for 10 genes in Xenopus laevis tadpoles (18 days postfertilization, PF) after acute exposure (2 days) to the PCB mixture Aroclor 1254. Specific gene expression signatures correlated with exposure and were predictive of adverse health effects. Exposure to low levels of Aroclor 1254 (5-50 ppb) significantly increased expression of six genes, independent of any health effects; exposure to midlevel concentrations (300-400 ppb) significantly decreased expression levels of two genes, NGF and beta-actin, prior to the onset of observable health effects; exposure to higher doses (500-700 ppb) significantly decreased NGF and beta-actin expression concomitant with the appearance of gross morphological abnormalities, behavioral deficits, and a statistically significant decrease in survival. This study expands upon our previous work that demonstrated an age-dependent susceptibility to Aroclor 1254 in Xenopus laevis tadpoles and that defined specific gene expression signatures as useful bioindicators of exposure and as predictors of overt or impending health effects.

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Section: Aroclor 1254 Alters Gross Morphology and Swimming Behaviorsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 64%

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Gene expression patterns predict exposure to PCBs in developing Xenopus laevis tadpoles

Jelaso

Lehigh-Shirey

Means

et al. 2003

Environ and Mol Mutagen

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“…Exposure at the higher concentrations (10, 50, and 100 μg/mL) caused statistically significant reductions in survival and body size, and resulted in histological abnormalities, including aberrant tail-tips, and aberrant myotomal and melanocyte morphologies; tadpoles treated with Aroclor 1254 were devoid of dendritic arborizations, resulting in decrease in total melanocyte area (Fisher et al, 2003).…”

Section: (B) Experimental Systems (I)mentioning

confidence: 99%

Polychlorinated Biphenyls and Polybrominated Biphenyls

Agudo

Aronson²,

Bonefeld-Jörgensen³

et al. 2008

Organic Pollutants

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initiated a programme on the evaluation of the carcinogenic risk of chemicals to humans involving the production of critically evaluated monographs on individual chemicals. The programme was subsequently expanded to include evaluations of carcinogenic risks associated with exposures to complex mixtures, lifestyle factors and biological and physical agents, as well as those in specific occupations. The objective of the programme is to elaborate and publish in the form of monographs critical reviews of data on carcinogenicity for agents to which humans are known to be exposed and on specific exposure situations; to evaluate these data in terms of human risk with the help of international working groups of experts in carcinogenesis and related fields; and to indicate where additional research efforts are needed. The lists of IARC evaluations are regularly updated and are available on the Internet at http:// monographs.iarc.fr/.This programme has been supported since 1982 by Cooperative Agreement U01 CA33193 with the United States National Cancer Institute, Department of Health and Human Services. Additional support has been provided since 1986 by the European Commission Directorate-General for Employment, Social Affairs, and Inclusion, initially by the Unit of Health, Safety and Hygiene at Work, and since 2014 by the European Union Programme for Employment and Social Innovation "EaSI" (2014-2020) The International Agency for Research on Cancer welcomes requests for permission to reproduce or translate its publications, in part or in full. Requests for permission to reproduce or translate IARC publications -whether for sale or for non-commercial distribution -should be addressed to the IARC Communications Group at: publications@iarc.fr.The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the Secretariat of the World Health Organization concerning the legal status of any country, territory, city, or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.The mention of specific companies or of certain manufacturers' products does not imply that they are endorsed or recommended by the World Health Organization in preference to others of a similar nature that are not mentioned. Errors and omissions excepted, the names of proprietary products are distinguished by initial capital letters.The IARC Monographs Working Group alone is responsible for the views expressed in this publication. IARC Library Cataloguing in Publication Data NOTE TO THE READERThe term 'carcinogenic risk' in the IARC Monographs series is taken to mean that an agent is capable of causing cancer. The Monographs evaluate cancer hazards, despite the historical presence of the word 'risks' in the title.Inclusion of an agent in the Monographs does not imply that it is a carcinogen, only that the published data have been examined. Equally, the fact that an agent has not yet been evaluated in a Monograph does not mean that...

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“…Due to their long half-lives, extreme persistence in environment and bio-accumulation capacity, PCBs exposure are thought to be a significant health hazard for human populations. Chronic exposure to PCBs and their metabolites have multifaceted adverse effects on wild organisms such as developmental deformities, [1,2] immunological defects, [3] endocrine disruption [4,5] and potential carcinogenesis. [6,7] Results of animal studies and epidemiological studies showed that PCBs could cause cancer development and enhance metastatic propensity in various target organs including liver, brain, breast, and prostate.…”

Section: Introductionmentioning

confidence: 99%

Aroclor 1254 inhibits cell viability and induces apoptosis of human A549 lung cancer cells by modulating the intracellular Ca²⁺level and ROS production through the mitochondrial pathway

Zhong

Guo

Wang

et al. 2015

Journal of Environmental Science and Health, Part A

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To study the acute toxic effects of PCBs on airway exposure, the cell viability, apoptosis and mitochondrial functions of human lung cancer cell line A549 were measured and compared after Aroclor 1254 exposure for different time. The results showed that Aroclor 1254 could inhibit cell viability and increase cell apoptosis in a concentration- and time-dependent manner. The mitochondrial apoptosis pathway was confirmed playing an important role. ROS elevation was an early response within 1h treatment of Aroclor 1254. Then after 4 h of Aroclor 1254 exposure, the intracellular calcium level increased and mitochondrial transmembrane potential (ΔΨm) collapsed, accompanying with Cytochrome c (Cyt-c) leakage, boosting expression of Bax, Apaf-1 and miRNA155, which were involved in the mitochondrial apoptosis pathway. After 24 h of Aroclor 1254 exposure, ROS returned to normal level, but cell apoptosis rate was higher than that at 4 h with ΔΨm continued collapsing and intracellular calcium increased. In conclusion, Aroclor 1254 could suppress cell viability and induce apoptosis in A549 cells, which was associated with ROS over-production and elevated cellular Ca(2+) level, which may result in mitochondrial dysfunction, inducing expression of Bax/Cyt-c/Apaf-1 and miRNA155.

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Exposure to the Polychlorinated Biphenyl Mixture Aroclor® 1254 Alters Melanocyte and Tail Muscle Morphology in Developing Xenopus Laevis Tadpoles

Cited by 7 publications

References 34 publications

Gene expression patterns predict exposure to PCBs in developing Xenopus laevis tadpoles

Gene expression patterns predict exposure to PCBs in developing Xenopus laevis tadpoles

Polychlorinated Biphenyls and Polybrominated Biphenyls

Aroclor 1254 inhibits cell viability and induces apoptosis of human A549 lung cancer cells by modulating the intracellular Ca²⁺level and ROS production through the mitochondrial pathway

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Exposure to the Polychlorinated Biphenyl Mixture Aroclor® 1254 Alters Melanocyte and Tail Muscle Morphology in Developing Xenopus Laevis Tadpoles

Cited by 7 publications

References 34 publications

Gene expression patterns predict exposure to PCBs in developing Xenopus laevis tadpoles

Gene expression patterns predict exposure to PCBs in developing Xenopus laevis tadpoles

Polychlorinated Biphenyls and Polybrominated Biphenyls

Aroclor 1254 inhibits cell viability and induces apoptosis of human A549 lung cancer cells by modulating the intracellular Ca2+level and ROS production through the mitochondrial pathway

Contact Info

Product

Resources

About

Aroclor 1254 inhibits cell viability and induces apoptosis of human A549 lung cancer cells by modulating the intracellular Ca²⁺level and ROS production through the mitochondrial pathway