Expressed Sequences from Conidial, Mycelial, and Sexual Stages ofNeurospora crassa

Nelson, Mary Anne; Kang, Seogchan; Braun, Edward L.; Crawford, Matthew E.; Dolan, Patricia; Léonard, Pascale M.; Mitchell, Jason W.; Armijo, Anne Marie; Bean, Laura E.; Blueyes, Eldon; Cushing, Thomas; Errett, Allison; Fleharty, Mark; Gorman, Marianita; Judson, Kimberly M.; Miller, Robert D.; Ortega, Jennifer; Pavlova, Iglika V.; Perea, John M.; Todisco, Shanda; Trujillo, Robert; Valentine, Joseph M.; Wells, Audrey; Werner‐Washburne, Margaret; Yazzie, Sheldwin; Natvig, Donald O.

doi:10.1006/fgbi.1997.0986

Cited by 141 publications

(123 citation statements)

References 39 publications

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“…The results demonstrated that all transcripts, and probably the encoded proteins, are expressed constitutively throughout the Neurospora life cycle (data not shown). This finding is corroborated by the identification of the various cDNA clones in three different cDNA libraries, representing both vegetative and sexual stages of development (30). 2 Characterization of NDE1 Activity-To characterize the activity of NDE1, mitochondria and IO-SMP were prepared from wild type and the nde-1 mutant and tested for different activities under various conditions.…”

Section: Resultsmentioning

confidence: 81%

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The External Calcium-dependent NADPH Dehydrogenase from Neurospora crassa Mitochondria

Melo

Duarte

Møller

et al. 2001

Journal of Biological Chemistry

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We have inactivated the nuclear gene coding for a putative NAD(P)H dehydrogenase from the inner membrane of Neurospora crassa mitochondria by repeat-induced point mutations. The respiratory rates of mitochondria from the resulting mutant (nde-1) were measured, using NADH or NADPH as substrates under different assay conditions. The results showed that the mutant lacks an external calcium-dependent NADPH dehydrogenase. The observation of NADH and NADPH oxidation by intact mitochondria from the nde-1 mutant suggests the existence of a second external NAD(P)H dehydrogenase. The topology of the NDE1 protein was further studied by protease accessibility, in vitro import experiments, and in silico analysis of the amino acid sequence. Taken together, it appears that most of the NDE1 protein extends into the intermembrane space in a tightly folded conformation and that it remains anchored to the inner mitochondrial membrane by an Nterminal transmembrane domain.In nonphotosynthetic eukaryotes, the mitochondrion is the cellular organelle responsible for producing most of the energy required for cellular metabolism. The process of oxidative phosphorylation takes place in the inner mitochondrial membrane, whereby the electrons produced by the oxidation of substrates like NAD(P)H are transported through the electron transport chain to oxygen, coupled to the generation of a transmembrane proton gradient that eventually leads to ATP synthesis (1). In contrast to mammals, the electron transport chains of plants and fungi possess several nonproton-pumping NAD(P)H dehydrogenases for transferring electrons to ubiquinone (2). In the case of potato tubers, four rotenone-insensitive NAD(P)H dehydrogenases have been identified in the inner mitochondrial membrane, two with the catalytic site facing the matrix (3, 4) and two facing the intermembrane space (5). In mitochondria from Saccharomyces cerevisiae, where the proton-pumping complex I is not present, the oxidation of NADH and NADPH is performed exclusively by three nonproton-pumping enzymes, one facing the matrix and two facing the intermembrane space (6 -8). In addition, the genome analysis of Synechocystis revealed three open reading frames that may code for such type II NAD(P)H dehydrogenases (9). On the other hand, only one external type II NADH dehydrogenase was reported for the fungus Yarrowia lipolytica (10). Although NAD(P)H dehydrogenases have been studied for a long time, our understanding of protein function at the molecular level is still very incomplete. The cloning of genes encoding several of these rotenoneinsensitive NAD(P)H dehydrogenases from mitochondria of different organisms (7, 10 -13) provides important tools for further research in this field. These enzymes might constitute a wasteful system acting to prevent the overreduction of the electron transport components and the production of reactive oxygen species, but their exact roles remain unclear.Both proton-pumping and nonproton-pumping NAD(P)H dehydrogenases have been described in Neurospora crassa mitochon...

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Section: Resultsmentioning

confidence: 81%

“…Northern blots (29) were hybridized with random primer-labeled restriction fragments from four N. crassa cDNA clones obtained from three cDNA libraries (30). The probes used were NM1C2, encoding NDE1, and SC3A5, SM1F6, and SP6B10, which encode the 12.3-, 17.8-, and 21-kDa subunits, respectively, of the mitochondrial complex I.…”

Section: Methodsmentioning

confidence: 99%

The External Calcium-dependent NADPH Dehydrogenase from Neurospora crassa Mitochondria

Melo

Duarte

Møller

et al. 2001

Journal of Biological Chemistry

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“…The first class contains genes similar to DHN-melanin PKS genes of the fungi Exophila dermatitidis 61 , Colletotrichum lagenarium 62 and Alternaria alternata 63 . Sequence identity to numerous expressed sequence tag (EST) sequences from sexual and perithecial libraries suggest a role in melanin pigment synthesis during sexual development 64 . The genes in the second class are similar in structure to several fungal PKSs, including the Aspergillus terreus lovF gene required for lovastatin synthesis.…”

Section: Polyketide Synthasesmentioning

confidence: 99%

The genome sequence of the filamentous fungus Neurospora crassa

Galagan

Calvo

Borkovich

et al. 2003

Nature

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Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report a high-quality draft sequence of the N. crassa genome. The approximately 40-megabase genome encodes about 10,000 protein-coding genes-more than twice as many as in the fission yeast Schizosaccharomyces pombe and only about 25% fewer than in the fruitfly Drosophila melanogaster. Analysis of the gene set yields insights into unexpected aspects of Neurospora biology including the identification of genes potentially associated with red light photobiology, genes implicated in secondary metabolism, and important differences in Ca(2+) signalling as compared with plants and animals. Neurospora possesses the widest array of genome defence mechanisms known for any eukaryotic organism, including a process unique to fungi called repeat-induced point mutation (RIP). Genome analysis suggests that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes

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“…The annual Neurospora bibliography (Strickland) provided another unparalleled data resource prior to the development of robust and open bibliographic databases such as Google Scholar. Shared data and molecular resources such as an restriction fragment length polymorphism mapping population and data (Nelson & Perkins 2000), gene (Nelson et al 1997), and genome libraries (Kelkar et al 2001) facilitated the development of one of the most densely populated genetic maps for all organisms (Barratt et al 1954a; Perkins et al 1969). Over many years, the genetic map along with characteristics of the mutants and mapping data have been published as a gene compendium (Perkins et al 1982, 2001).…”

Section: Introductionmentioning

confidence: 99%

Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era

McCluskey

Baker

2017

Mycology

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Filamentous fungi have been important as model organisms since the beginning of modern biological inquiry and have benefitted from open data since the earliest genetic maps were shared. From early origins in simple Mendelian genetics of mating types, parasexual genetics of colony colour, and the foundational demonstration of the segregation of a nutritional requirement, the contribution of research systems utilising filamentous fungi has spanned the biochemical genetics era, through the molecular genetics era, and now are at the very foundation of diverse omics approaches to research and development. Fungal model organisms have come from most major taxonomic groups although Ascomycete filamentous fungi have seen the most major sustained effort. In addition to the published material about filamentous fungi, shared molecular tools have found application in every area of fungal biology. Similarly, shared data has contributed to the success of model systems. The scale of data supporting research with filamentous fungi has grown by 10 to 12 orders of magnitude. From genetic to molecular maps, expression databases, and finally genome resources, the open and collaborative nature of the research communities has assured that the rising tide of data has lifted all of the research systems together.

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Expressed Sequences from Conidial, Mycelial, and Sexual Stages ofNeurospora crassa

Cited by 141 publications

References 39 publications

The External Calcium-dependent NADPH Dehydrogenase from Neurospora crassa Mitochondria

The External Calcium-dependent NADPH Dehydrogenase from Neurospora crassa Mitochondria

The genome sequence of the filamentous fungus Neurospora crassa

Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era

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