Expressing and functional analysis of mammalian apoptotic regulators in yeast

Greenwood, Michael T.; Ludovico, Paula

doi:10.1038/cdd.2009.177

Cited by 36 publications

(22 citation statements)

References 95 publications

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“…A variety of yeast genes and proteins, orthologues of mammalian apoptosis regulators, were shown to play a role in yeast PCD triggered by a variety of either endogenous or exogenous stimuli, including harsh chemical treatment, gene mutations, aging, and heterologous expression of human pro-apoptotic proteins [3,6]. Accordingly, some processes occurring in mammalian apoptosis take place, sometimes with some difference, in yeast PCD.…”

Section: Introductionmentioning

confidence: 98%

Achievements and perspectives in yeast acetic acid-induced programmed cell death pathways

Guaragnella

Antonacci

Passarella

et al. 2011

Biochemical Society Transactions

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The use of non-mammalian model organisms, including yeast Saccharomyces cerevisiae, can provide new insights into eukaryotic PCD (programmed cell death) pathways. In the present paper, we report recent achievements in the elucidation of the events leading to PCD that occur as a response to yeast treatment with AA (acetic acid). In particular, ROS (reactive oxygen species) generation, cyt c (cytochrome c) release and mitochondrial function and proteolytic activity will be dealt with as they vary along the AA-PCD time course by using both wild-type and mutant yeast cells. Two AA-PCD pathways are described sharing common features, but distinct from one another with respect to the role of ROS and mitochondria, the former in which YCA1 acts upstream of cyt c release and caspase-like activation in a ROS-dependent manner and the latter in which cyt c release does not occur, but caspase-like activity increases, in a ROS-independent manner.

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Section: Introductionmentioning

confidence: 98%

Achievements and perspectives in yeast acetic acid-induced programmed cell death pathways

Guaragnella

Antonacci

Passarella

et al. 2011

Biochemical Society Transactions

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“…For this reason, yeast PCD is often referred to as "yeast apoptosis". However, yeast PCD does not utilize all of the same regulators as mammalian apoptosis nor the extensive family of caspases typical of metazoan apoptosis 9697. In this review, to avoid semantic distinctions, I refer to yeast apoptosis as PCD.…”

Section: Varying Fates Varying Functionsmentioning

confidence: 99%

Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation

Honigberg¹

2016

Microbial Cell

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Diploid budding yeast (Saccharomyces cerevisiae) can adopt one of several alternative differentiation fates in response to nutrient limitation, and each of these fates provides distinct biological functions. When different strain backgrounds are taken into account, these various fates occur in response to similar environmental cues, are regulated by the same signal transduction pathways, and share many of the same master regulators. I propose that the relationships between fate choice, environmental cues and signaling pathways are not Boolean, but involve graded levels of signals, pathway activation and master-regulator activity. In the absence of large differences between environmental cues, small differences in the concentration of cues may be reinforced by cell-to-cell signals. These signals are particularly essential for fate determination within communities, such as colonies and biofilms, where fate choice varies dramatically from one region of the community to another. The lack of Boolean relationships between cues, signaling pathways, master regulators and cell fates may allow yeast communities to respond appropriately to the wide range of environments they encounter in nature.

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“…Due to the lack of obvious counterparts (e.g., p53, Bcl-2), many early studies considered yeast an in vivo test tube in which to analyze mammalian apoptotic regulators free of complications from yeast-based PCD (Manon et al 1997;Ligr et al 1998;Lisa-Santamaria et al 2009;Greenwood and Ludovico 2010;reviewed in Silva et al 2011;Clapp et al 2012). However, extensive studies in budding yeast, fission yeast, and other single-cell eukaryotes challenge this view (Shemarova 2010).…”

Section: Executioners Of the Programmed Cell Death Pathwaymentioning

confidence: 99%

Programmed Cell Death Initiation and Execution in Budding Yeast

Strich

2015

Genetics

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Apoptosis or programmed cell death (PCD) was initially described in metazoans as a genetically controlled process leading to intracellular breakdown and engulfment by a neighboring cell . This process was distinguished from other forms of cell death like necrosis by maintenance of plasma membrane integrity prior to engulfment and the well-defined genetic system controlling this process. Apoptosis was originally described as a mechanism to reshape tissues during development. Given this context, the assumption was made that this process would not be found in simpler eukaryotes such as budding yeast. Although basic components of the apoptotic pathway were identified in yeast, initial observations suggested that it was devoid of prosurvival and prodeath regulatory proteins identified in mammalian cells. However, as apoptosis became extensively linked to the elimination of damaged cells, key PCD regulatory proteins were identified in yeast that play similar roles in mammals. This review highlights recent discoveries that have permitted information regarding PCD regulation in yeast to now inform experiments in animals.

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Expressing and functional analysis of mammalian apoptotic regulators in yeast

Cited by 36 publications

References 95 publications

Achievements and perspectives in yeast acetic acid-induced programmed cell death pathways

Achievements and perspectives in yeast acetic acid-induced programmed cell death pathways

Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation

Programmed Cell Death Initiation and Execution in Budding Yeast

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