Expression analysis and promoter methylation under osmotic and salinity stress of TaGAPC1 in wheat (Triticum aestivum L)

Abstract

Cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) catalyzes a key reaction in glycolysis and encoded by a multi-gene family which showed instability expression under abiotic stress. DNA methylation is an epigenetic modification that plays an important role in gene regulation in response to abiotic stress. The comprehension of DNA methylation at promoter region of TaGAPC1 can provide insights into the transcription regulation mechanisms of plant genes under abiotic stress. In this study, we cloned TaGAPC1 genes and its promoters from two wheat genomes, then investigated the expression patterns of TaGAPC1 under osmotic and salinity stress, and analyzed the promoter sequences. Moreover, the methylation patterns of promoters under stress were confirmed. Expression analysis indicated that TaGAPC1 was induced inordinately by stresses in two wheat genotypes with contrasting drought tolerance. Several stress-related cis-acting elements (MBS, DRE, GT1 and LTR et al.) were located in its promoters. Furthermore, the osmotic and salinity stress induced the demethylation of CG and CHG nucleotide in the promoter region of Changwu134. The methylation level of CHG and CHH in promoter of Zhengyin1 was always increased under stresses, and the CG contexts remained unchanged. The cytosine loci of stress-related cis-acting elements also showed different methylation changes in this process. These results provide insights into the relationship between promoter methylation and gene expression, promoting the function investigation of GAPC.

Salt stress induces genotype-specific DNA hypomethylation in ZmEXPB2 and ZmXET1 genes in maize

“…This genotype specific high level of DNA methylation is in accordance with the previous reports where DNA hypermethylation has been observed stress responsive genes under control conditions. In a recent study, high level of DNA methylation was revealed in ABRERATCAL and MYB-core cis-regulatory elements of the promoter region of TaGAPC1 in wheat (Fei et al 2017). Similar to our results, the relatively more tolerant wheat genotype showed higher level of DNA methylation under normal conditions.…”

Comprehensive In Silico Characterization and Expression Profiling of TCP Gene Family in Rapeseed

“…In wheat, the expression pattern of TaGAPC1 was regulated under osmotic and salinity stress conditions. The promoter region of TaGAPC1 contains various stress-related cis -regulatory elements (including MBS, DRE, GT1, and LTR), and methylation changes were observed during the stress treatments, leading to stress tolerance and regulation in gene expression ( Fei et al, 2017 ). In the current study, these results were further authenticated by the GO annotation evaluation.…”

Chitooligosaccharides enhance cold tolerance by repairing photodamaged PS II in rice

“…Improving the quality and biotic/abiotic resistances of crops has become a topic of considerable interest throughout the world. Progress in breeding biotic and abiotic stress-resistant crops and developing biotic and abiotic stress resistance-inducing reagents, especially oligosaccharide-like, environmentally friendly reagents, has been achieved (Thomashow, 1999; Dzung et al ., 2011; Liu et al ., 2013; Sun et al ., 2013; Qiang et al ., 2015; Jing et al ., 2016; Fei et al ., 2017).…”