Expression analysis of facilitative glucose transporters (GLUTs) in human thyroid carcinoma cell lines and primary tumors

Abstract

Expression analysis of facilitative glucose transporters (GLUTs) in human thyroid carcinoma cell lines and primary tumors, Molecular and Cellular Endocrinology (2007), doi:10.1016/j.mce.2008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. We studied several GLUT isoforms expression in thyroid tumor cell lines deriving from anaplastic (ARO, FRO), papillary (NPA), follicular (WRO) and medullary (TT) human thyroid carcinoma. GLUT1 and GLUT3 were also studied in 157 human thyroid malignant and benign tissues.Quantitative Real-time RT-PCR analysis revealed that GLUT1 mRNA levels were higher in less-differentiated cells (ARO, FRO) while GLUT3 mRNA levels were prevalent in well-differentiated cells (NPA, WRO). Accordingly, Western blot showed high expression and correct membrane targeting of GLUT1 protein in ARO and FRO and of GLUT3 protein in NPA and WRO. All cell lines were able to take-up different rates of 3 H-Deoxy-Glucose. The analysis of GLUT1 and GLUT3 mRNA expression in human thyroid tissues showed the prevalence of GLUT1, but not of GLUT3, in malignant with respect to normal tissues. Finally, both GLUT1 and GLUT3 showed a slightly higher expression in anaplastic than in well-differentiated tumors.In conclusion, we showed that GLUT1 and GLUT3 were the most important glucose transporters in the thyroid tumoral cells. In particular GLUT1 was the most prevalent in less-differentiated cells (ARO and FRO) while GLUT3 was the most prevalent in welldifferentiated cells (NPA and WRO). A similar pattern of expression was found for GLUT1 but not for GLUT3 in human thyroid tumors.

Regulation of Uptake of18F-FDG by a Follicular Human Thyroid Cancer Cell Line with Mutation-Activated K-Ras

“…Ninety-five percent of human follicular carcinomas are deficient for GLUT-1 messenger RNA (23). This observation is not completely unexpected and also agrees with results obtained by Ciampi et al, who reported an overexpression of GLUT-3 in other well-differentiated follicular thyroid carcinoma cell lines (24).…”

PTEN regulates plasma membrane expression of glucose transporter 1 and glucose uptake in thyroid cancer cells

“…Thyroid cancer cells also show a high rate of glycolysis (Andrade et al 2012). Immunohistochemical studies have shown that enhanced glucose uptake in cancer cells correlates with over-expression of GLUTs, in most cases GLUT1 (Haber et al 1997, Ciampi et al 2008. Herein, we show that the oncosuppressor PTEN plays a dominant role in the membrane expression of GLUT1 and glucose uptake in thyroid cancer cells.…”

The role of 18F-fluorodeoxyglucose positron emission tomography in differentiated thyroid cancer before surgery

“…However, the expression of GLUT3 (SLC2A3) can be observed in DTCs (Ciampi et al 2008), and correlations between GLUT3, GLUT4 (SLC2A4), and SUV were significant in thyroid cancer (Kaida et al 2011). Therefore, FDG uptake may be determined by the expression of GLUT3 and GLUT4 (Kaida et al 2011).…”