Expression analysis of genes for cytochrome P450 CYP86 and glycerol-3-phosphate acyltransferase related to suberin biosynthesis in rice roots under stagnant deoxygenated conditions

Nishiuchi, Shunsaku; Watanabe, Kohtaro; Sato, Saori; Takahashi, Hirokazu; Nakazono, Mikio

doi:10.3117/plantroot.15.19

Cited by 8 publications

(9 citation statements)

References 43 publications

(48 reference statements)

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“…It has been suggested that phenylpropanoids, such as suberin or lignin, are the major components of the ROL barrier, although it is not clear which one has a more significant role (Kotula et al ., 2009). Some genes were commonly upregulated under H 2 S and stagnant conditions, and different copies of suberin biosynthesis genes like KCS , GPAT , CYP86A were upregulated in both conditions (Table S7), and two GPAT genes ( LOC_Os02g02340 and LOC_05g38350 ) have been shown to be specifically expressed in the exodermis (Nishiuchi et al ., 2021). Previous studies have identified a number of genes related to suberin biosynthesis responding to stagnant conditions (Kulichikhin et al ., 2014; Shiono et al ., 2014).…”

Section: Discussionmentioning

confidence: 99%

The barrier to radial oxygen loss protects roots against hydrogen sulphide intrusion and its toxic effect

et al. 2023

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Summary The root barrier to radial O2 loss (ROL) is a key root trait preventing O2 loss from roots to anoxic soils, thereby enabling root growth into anoxic, flooded soils. We hypothesized that the ROL barrier can also prevent intrusion of hydrogen sulphide (H2S), a potent phytotoxin in flooded soils. Using H2S‐ and O2‐sensitive microsensors, we measured the apparent permeance to H2S of rice roots, tested whether restricted H2S intrusion reduced its adverse effects on root respiration, and whether H2S could induce the formation of a ROL barrier. The ROL barrier reduced apparent permeance to H2S by almost 99%, greatly restricting H2S intrusion. The ROL barrier acted as a shield towards H2S; O2 consumption in roots with a ROL barrier remained unaffected at high H2S concentration (500 μM), compared to a 67% decline in roots without a barrier. Importantly, low H2S concentrations induced the formation of a ROL barrier. In conclusion, the ROL barrier plays a key role in protecting against H2S intrusion, and H2S can act as an environmental signalling molecule for the induction of the barrier. This study demonstrates the multiple functions of the suberized/lignified outer part of the rice root beyond that of restricting ROL.

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Section: Discussionmentioning

confidence: 99%

The barrier to radial oxygen loss protects roots against hydrogen sulphide intrusion and its toxic effect

et al. 2023

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“…The spatial expression patterns of OsCYP86A9 coincided with the patterns of suberin formation in rice roots (Nishiuchi et al, 2021). To determine if OsCYP86A9 plays a role in suberin formation, we stained the root sections of WT and oscyp86a9 mutant plants with Sudan Red 7B, that stains suberin in red color (Brundrett et al, 1991).…”

Section: Osmyb80 Directly Regulates Oscyp86a9 Expression In Anthersmentioning

confidence: 99%

“…The rice genome contains only one ortholog of AtCYP86A1, i.e., OsCYP86A9. Previous studies showed that OsCYP86A9 was specifically expressed in the root exodermis cells 10-35 mm away from the root apex (Nishiuchi et al, 2021). Rice plants have been shown to remarkably enhance root suberization to develop a physical barrier against radial oxygen loss under stagnant deoxygenated conditions (Ranathunge et al, 2011).…”

Section: Oscyp86a9 Is Crucial In Root Suberin Depositionmentioning

confidence: 99%

“…In response to deoxygenated stagnant conditions, more suberin is synthesized and deposited in the outer layers of the roots (Kotula et al, 2009). Previous studies have shown a significant upregulation of OsCYP86A9, OsCYP86A10, OsCYP86A11, and OsCYP86B3 were in the adventitious roots of rice grown under stagnant deoxygenated conditions (Nishiuchi et al, 2021;Shiono et al, 2014). Moreover, OsCYP86A9, OsCYP86A11, and OsCYP86B3 were mainly expressed in root exodermis cells (Nishiuchi et al, 2021).…”

Section: Introductionmentioning

confidence: 98%

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Regulation by distinct MYB transcription factors defines the roles of OsCYP86A9 in anther development and root suberin deposition

Huang,

Li,

Chang

et al. 2024

The Plant Journal

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SUMMARYCytochrome P450 proteins (CYPs) play critical roles in plant development and adaptation to fluctuating environments. Previous reports have shown that CYP86A proteins are involved in the biosynthesis of suberin and cutin in Arabidopsis. However, the functions of these proteins in rice remain obscure. In this study, a rice mutant with incomplete male sterility was identified. Cytological analyses revealed that this mutant was defective in anther development. Cloning of the mutant gene indicated that the responsible mutation was on OsCYP86A9. OsMYB80 is a core transcription factor in the regulation of rice anther development. The expression of OsCYP86A9 was abolished in the anther of osmyb80 mutant. In vivo and in vitro experiments showed that OsMYB80 binds to the MYB‐binding motifs in OsCYP86A9 promoter region and regulates its expression. Furthermore, the oscyp86a9 mutant exhibited an impaired suberin deposition in the root, and was more susceptible to drought stress. Interestingly, genetic and biochemical analyses revealed that OsCYP86A9 expression was regulated in the root by certain MYB transcription factors other than OsMYB80. Moreover, mutations in the MYB genes that regulate OsCYP86A9 expression in the root did not impair the male fertility of the plant. Taken together, these findings revealed the critical roles of OsCYP86A9 in plant development and proposed that OsCYP86A9 functions in anther development and root suberin formation via two distinct tissue‐specific regulatory pathways.

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“…Cell wall depositions of suberin and/or lignin in the outer part of the root are thought to form the functional biochemical components of the barrier (Schreiber 1999;Kotula et al 2009;Watanabe et al 2017). Suberin is mainly deposited in the exodermal cell walls (Kotula et al 2009;Nishiuchi et al 2021), whereas lignin is mainly found in the sclerenchyma (Armstrong 2000;Soukup et al 2007). The molecular mechanisms controlling barrier formation have not yet been fully elucidated.…”

Section: Formation Of a Root Barrier To Radial O 2 Lossmentioning

confidence: 99%

Flooding-adaptive root and shoot traits in rice

Lin,

Zhang,

Shen

et al. 2024

Funct. Plant Biol.

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Wetland plants, including rice (Oryza spp.), have developed multiple functional adaptive traits to survive soil flooding, partial submergence or even complete submergence. In waterlogged soils and under water, diffusion of O2 and CO2 is extremely slow with severe impacts on photosynthesis and respiration. As a response to shallow floods or rising floodwater, several rice varieties, including deepwater rice, elongate their stems to keep their leaves above the water surface so that photosynthesis can occur unhindered during partial submergence. In stark contrast, some other varieties hardly elongate even if they become completely submerged. Instead, their metabolism is reduced to an absolute minimum so that carbohydrates are conserved enabling fast regrowth once the floodwater recedes. This review focuses on the fascinating functional adaptive traits conferring tolerance to soil flooding, partial or complete submergence. We provide a general analysis of these traits focusing on molecular, anatomical and morphological, physiological and ecological levels. Some of these key traits have already been introgressed into modern high-yielding genotypes improving flood tolerance of several cultivars used by millions of farmers in Asia. However, with the ongoing changes in climate, we propose that even more emphasis should be placed on improving flood tolerance of rice by breeding for rice that can tolerate longer periods of complete submergence or stagnant flooding. Such tolerance could be achieved via additional tissues; i.e. aquatic adventitious roots relevant during partial submergence, and leaves with higher underwater photosynthesis caused by a longer gas film retention time.

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Expression analysis of genes for cytochrome P450 CYP86 and glycerol-3-phosphate acyltransferase related to suberin biosynthesis in rice roots under stagnant deoxygenated conditions

Cited by 8 publications

References 43 publications

The barrier to radial oxygen loss protects roots against hydrogen sulphide intrusion and its toxic effect

The barrier to radial oxygen loss protects roots against hydrogen sulphide intrusion and its toxic effect

Regulation by distinct MYB transcription factors defines the roles of OsCYP86A9 in anther development and root suberin deposition

Flooding-adaptive root and shoot traits in rice

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