Expression analysis of integrin β1 isoforms during zebrafish embryonic development

Wang, Xin; Li, Liping; Liu, Dong

doi:10.1016/j.gep.2014.10.001

Cited by 10 publications

(13 citation statements)

References 26 publications

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“…We confirmed integrin β1b expression pattern by a whole‐mount in situ hybridization analysis and showed that integrin β1b transcript is expressed in the whole embryo, including an intermediate cellular mass (ICM), where vasculature is generated, and the dorsal midline junction in the head (Supporting Information Figure S1), in accordance with previous reports (Wang et al, ; Wu et al, ). To investigate the functions of integrin β1b in embryogenesis, we obtained and observed an integrin β1b mi371 mutant (Figure a).…”

Section: Resultssupporting

confidence: 91%

“…Zebrafish represent one of the most versatile vertebrate models and are particularly useful for live‐cell imaging because of their external fertilization, rapid development and transparency during embryogenesis (Kimmel, Ballard, Kimmel, Ullmann, & Schilling, ). Due to an ancestral whole‐genome duplication (Woods et al, ), zebrafish have four integrin β1 orthologous genes, β1a , β1b, β1b.1 and β1b.2 (Mould et al, ; Wang, Li, & Liu, ). These paralogous genes are expressed in multiple organs including the heart, myotome and epithelium during development (Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Due to an ancestral whole‐genome duplication (Woods et al, ), zebrafish have four integrin β1 orthologous genes, β1a , β1b, β1b.1 and β1b.2 (Mould et al, ; Wang, Li, & Liu, ). These paralogous genes are expressed in multiple organs including the heart, myotome and epithelium during development (Wang et al, ). Another study also indicated integrin β1b expression in the axial vessels and heart tube, and conferred its cardiovascular functions assayed by antisense morpholino‐mediated knockdown (Wu et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Integrin β1 activity is required for cardiovascular formation in zebrafish

et al. 2018

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Integrins are transmembrane molecules that facilitate cell-to-cell and cell-to-extracellular matrix (ECM) interactions. Integrin molecules are heterodimers that consist of α- and β-subunits. The integrin β1 gene is widely expressed in vivo and is the major β molecule in many tissues; however, tissue-specific roles of integrin β1 are still elusive. In this study, we investigated integrin β1 function in endothelial cells of zebrafish. An integrin β1b mutant zebrafish exhibited morphological abnormalities in blood vessel formation, cephalic hemorrhage and a decreased responsiveness to tactile stimulation during development. To determine the role of integrin β1b in vascular formation, we developed a Gal4/UAS-mediated conditional inactivation of integrin β1 by expressing the cytoplasmic region of integrin β1 that acts as a dominant-negative (DN) isoform. Expression of integrin β1 DN in endothelial cells induced blood vessel abnormalities as in integrin β1b mutants. These results show that endothelial cells require integrin activity for the formation and/or maintenance of blood vessels in zebrafish. Furthermore, our time-lapse recording visualized the breakpoint of cephalic vessels and the hemorrhage onset. Taken together, our tissue-specific inactivation of integrin β1 in zebrafish is powerful tools for functional analysis of integrin β1 in developing tissues.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrin β1 activity is required for cardiovascular formation in zebrafish

et al. 2018

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“…Then we did a series of experiments to testify weather integrin β1 was the receptor through which Maeg regulates angiogenesis in zebrafish embryos. Based on the previous studies, there are four itgb1 isoforms in zebrafish, including itgb1a , itgb1b , itgb1b.1 , and itgb1b.2 [30, 31]. We isolated GFP+ cells from Tg(kdrl:EGFP) transgenic embryos at 24 hpf by fluorescence-activated cell sorting (FACs) (Supplementary Figure S7A).…”

Section: Resultsmentioning

confidence: 99%

“…The detailed information of these probes and the primers for generating these probes are listed in supplemental data (Table S1) or described in previous work [22, 30, 40]. DIG-labeled RNA sense and antisense probes were made from the linearized plasmids according to the manufacturer's protocol using the DIG RNA Labeling Kit (SP6/T7) (Roche).…”

Section: Methodsmentioning

confidence: 99%

The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis

et al. 2016

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MAM and EGF containing gene (MAEG), also called Epidermal Growth Factor-like domain multiple 6 (EGFL6), belongs to the epidermal growth factor repeat superfamily. The role of Maeg in zebrafish angiogenesis remains unclear. It was demonstrated that maeg was dynamically expressed in zebrafish developing somite during a time window encompassing many key steps in embryonic angiogenesis. Maeg loss-of-function embryos showed reduced endothelial cell number and filopodia extensions of intersegmental vessels (ISVs). Maeg gain-of-function induced ectopic sprouting evolving into a hyperbranched and functional perfused vasculature. Mechanistically we demonstrate that Maeg promotes angiogenesis dependent on RGD domain and stimulates activation of Akt and Erk signaling in vivo. Loss of Maeg or Itgb1, augmented expression of Notch receptors, and inhibiting Notch signaling or Dll4 partially rescued angiogenic phenotypes suggesting that Notch acts downstream of Itgb1. We conclude that Maeg acts as a positive regulator of angiogenic cell behavior and formation of functional vessels.

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Egfl6 is involved in zebrafish notochord development

Wang

Wei

et al. 2015

Fish Physiol Biochem

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The epidermal growth factor (EGF) repeat motif defines a superfamily of diverse protein involved in regulating a variety of cellular and physiological processes, such as cell cycle, cell adhesion, proliferation, migration, and neural development. Egfl6, an EGF protein, also named MAGE was first cloned in human tissue. Up to date, the study of zebrafish Egfl6 expression pattern and functional analysis of Egfl6 involved in embryonic development of vertebrate in vivo is thus far lacking. Here we reported that Egfl6 was involved in zebrafish notochord development. It was shown that Egfl6 mRNA was expressed in zebrafish, developing somites, fin epidermis, pharyngeal arches, and hindbrain region. Particularly the secreted Egfl6 protein was significantly accumulated in notochord. Loss of Egfl6 function in zebrafish embryos resulted in curved body with distorted notochord in the posterior trunk. It was observed that expression of all Notch ligand and receptors in notochord of 28 hpf Egfl6 morphants was not affected, except notch2, which was up-regulated. We found that inhibition of Notch signaling by DAPT efficiently rescued notochord developmental defect of Egfl6 deficiency embryos.

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Expression analysis of integrin β1 isoforms during zebrafish embryonic development

Cited by 10 publications

References 26 publications

Integrin β1 activity is required for cardiovascular formation in zebrafish

Integrin β1 activity is required for cardiovascular formation in zebrafish

The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis

Egfl6 is involved in zebrafish notochord development

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