Expression Analysis of the LFY and TFL1 Homologs in Floral Buds of Japanese Pear (Pyrus pyrifolia Nakai) and Quince (Cydonia oblonga Mill.)

Esumi, Tomoya; Tao, Ryutaro; Yonemori, Keizo

doi:10.2503/jjshs1.77.128

Cited by 10 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar to peach, TFL1 expression was absent or low during floral differentiation in buds of apple (Hattasch et al, 2008;Mimida et al, 2009Mimida et al, , 2011, quince [C. oblonga (Esumi et al, 2008)], and rose [Rosa wichurana (Iwata et al, 2012)]. However, in pear (P. pyrifolia, P. communis), one of the two TFL1 genes was expressed during floral differentiation (Esumi et al, 2005(Esumi et al, , 2008.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Peach TFL1 and Comparison with FT/TFL1 Gene Families of the Rosaceae

Chen¹,

Jiang²,

Thammannagowda³

et al. 2013

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

We investigated the FT/TFL1 family of peach (Prunus persica), a gene family that regulates floral induction in annual and perennial plants. The peach terminal flower 1 gene (PpTFL1) was expressed in a developmental and tissue-specific pattern that, overall, was similar to that of TFL1 orthologs in other woody Rosaceae species. Consistent with a role as a floral inhibitor, ectopic expression of PpTFL1 in arabidopsis (Arabidopsis thaliana) delayed flowering and prolonged vegetative growth. Other members of the peach FT/TFL1 family were identified from the sequenced genome, including orthologs of flowering locus T, centroradialis, brother of ft, and mother of ft and tfl. Sequence analysis found that peach FT/TFL1 family members were more similar to orthologous genes across the Rosaceae than to each other. Together these results suggest that information on genes that regulate flowering in peach could be applied to other Rosaceae species, particularly ornamentals.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of Peach TFL1 and Comparison with FT/TFL1 Gene Families of the Rosaceae

Chen¹,

Jiang²,

Thammannagowda³

et al. 2013

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

show abstract

“…Kandidat lain yang dapat digunakan adalah gen TERMINAL FLOWER1 (TFL1 dan TLP2) dari Arabidopsis yang dapat membuat fenotipe bunga bergerombol pada ujung cabang dan tanaman berbunga berhenti di ujung cabang (determinate) sehingga panennya serempak (Esumi et al 2008). Dengan berkembangnya informasi sekuen genom rujukan jarak pagar, ortolog gen serupa semestinya juga dapat diisolasi dari genom jarak pagar sendiri sehingga lebih cocok untuk dimanipulasi melalui teknik rekayasa genetika.…”

Section: Perakitan Bahan Tanaman Dengan Buah Matang Serempakunclassified

Pendekatan Bioteknologi dan Genomika untuk Perbaikan Genetik Tanaman Jarak Pagar sebagai Penghasil Bahan Bakar Nabati

Tasma

2018

J. AgroBiogen

View full text Add to dashboard Cite

Physic nut (Jatropha curcas L.) is an oil-bearing crop whose oil can be used as diesel substitution. This crop can be grown at suboptimal soils and thus attracts many people to explore its potential as a biorenewable energy crop. Many constrains, however, are faced by this crop. From plant material and cultivation aspects, not much information has yet been known regarding this crop. In addition, people still view physic nut as an incomplete domesticated crop as indicated by the fact that many J. curcas accessions of the world still have toxic in their seeds causing the protein-rich seeds can not directly be used as animal feed. J. curcas demonstrates continuous fruiting habit during its fruiting season and hence no uniform fruit maturity causing expensive harvesting cost. The female and male flower ratio is low causing lower seed productivity. J. curcas oil is relatively rich with poly unsaturated fatty acids that need to be decreased to improve diesel quality. Genomic knowledge to understand J. curcas genes and function is critical to manipulate J. curcas genetics systematically. Genetic transformation technology is potentially applied for better plant architecture, improvement of agronomically important characters, seed and oil yield, and quality traits. The objective of this manuscript was to review biotechnology and genomic approaches for genetic improvement of J. curcas plant materials. The biotechnological method application should expedite J. curcas breeding programs. With superior planting materials, the J. curcas should be able to be cultivated economically and resulting better J. curcas oil suitable for biodiesel industry.Keywords: Jatropha curcas, genomics, gene and QTL, genetic transformation, marker-assisted breeding. ABSTRAKJarak pagar (Jatropha curcas L.) merupakan tanaman penghasil minyak nabati yang dapat digunakan sebagai pengganti minyak diesel. Tanaman yang dapat tumbuh pada kondisi lahan kurang subur ini menarik minat banyak pihak untuk mengekplorasi potensinya sebagai tanaman sumber energi yang ramah lingkungan. Namun, masih banyak kendala yang dihadapi dalam pembudidayaannya supaya dapat diusahakan secara ekonomis. Dari aspek bahan tanaman dan budi daya, saat ini tanaman jarak pagar masih belum banyak diketahui. Bahkan, jarak pagar masih dianggap sebagai tanaman yang belum didomestikasikan secara penuh seperti ditunjukkan oleh fakta bahwa sebagian besar genotipe jarak pagar di dunia bijinya toksik sehingga ampas bijinya yang kaya protein tidak dapat langsung digunakan sebagai pakan ternak. Kematangan buah tanaman ini tidak serempak yang menyebabkan biaya panen tinggi. Rasio bunga betina dan bunga jantan yang rendah menyebabkan produktivitas bijinya rendah. Biji jarak pagar mengandung asam lemak poli tidak jenuh yang konsentrasinya perlu diturunkan untuk meningkatkan mutu minyak diesel. Pengetahuan genomika memungkinkan untuk mengetahui komposisi genom, komposisi dan fungsi gen, dan pemetaan genetik (gen/QTL) unggul jarak pagar. Pemahaman ini diperlukan agar genetika tanaman jarak...

show abstract

“…The AFL genes have traits in common with the Arabidopsis thaliana LEAFY (LFY) and snapdragon, Antirrhinum majus, FLORICAULA (FLO) as the floral meristem identity gene (Coen et al, 1990;Wada et al, 2002;Weigel et al, 1992). The homologues from other herbaceous plants (Bomblies et al, 2003;Hofer et al, 1997;Kelly et al, 1995;MolineroRosales et al, 1999), woody plants (Mouradov et al, 1998;Rottman et al, 2000) and fruit trees (Carmona et al, 2002;Esumi et al, 2007;Esumi et al, 2008;Walton et al, 2001;Yu et al, 2005) are thought to be involved in the flowering events in a similar manner. The expression of AFL1 and AFL2 in Arabidopsis promotes flowering.…”

Section: Introductionmentioning

confidence: 99%

Anatomical Analysis by Two Approaches Ensure the Promoter Activities of Apple AFL Genes

Wada

Ureshino

Tanaka

et al. 2009

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

The apple AFL (Apple FLORICAULA LEAFY) 1 and 2 gene promoter linked β-glucuronidase (GUS) clearly displayed staining at the meristems of the shoot apexes, lateral axils, and leaf primordia in apple trees. The GUS staining of AFL1 promoter revealed that the AFL1 gene was also expressed at a vegetative meristem, and the staining patterns of AFL2 promoter were almost the same in the culture shoots and two-year-old trees. Quantitative RT-PCR analysis also showed that both genes were expressed at floral buds, where the expression of each was higher than the vegetative expression individually and increased with floral developments. These results showed the similarity in the expression patterns of the two AFL genes, and this expression was not affected by the vegetative growth condition, but was regulated at floral development. In situ hybridization of AFL genes supported the results of the GUS staining. The specific probe of each AFL gene showed almost the same expression pattern at the meristem of shoot apexes, lateral axils, and leaf primordia from the seedling, culture shoots, and watersprouts. These results indicated that the promoter GUS analysis and the in situ hybridization can be combined for the analysis of AFL gene expression. The cooperative usage of the GUS analysis and in situ hybridization suggested that they are powerful tools for apple promoter analysis.

show abstract

Expression Analysis of the LFY and TFL1 Homologs in Floral Buds of Japanese Pear (Pyrus pyrifolia Nakai) and Quince (Cydonia oblonga Mill.)

Cited by 10 publications

References 22 publications

Characterization of Peach TFL1 and Comparison with FT/TFL1 Gene Families of the Rosaceae

Characterization of Peach TFL1 and Comparison with FT/TFL1 Gene Families of the Rosaceae

Pendekatan Bioteknologi dan Genomika untuk Perbaikan Genetik Tanaman Jarak Pagar sebagai Penghasil Bahan Bakar Nabati

Anatomical Analysis by Two Approaches Ensure the Promoter Activities of Apple AFL Genes

Contact Info

Product

Resources

About