Expression and Cellular Distribution of INHA and INHB before and after<i>In Vitro</i>Cultivation of Porcine Oocytes Isolated from Follicles of Different Size

Kempisty, Bartosz; Jackowska, M.; Woźna, M.; Antosik, Paweł; Piotrowska, H.; Zawierucha, Piotr; Bukowska, Dorota; Jaśkowski, J. M.; Nowicki, Michał; Brüssow, Klaus‐Peter

doi:10.1155/2012/742829

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…In relation to the INHBA gene, previous studies have demonstrated a higher expression in porcine MF‐derived oocytes, than in those derived from SF after IVM (Kempisty et al., 2012) and in protein prior to and after IVM (Kempisty et al., 2015); similar findings were described in the case of MII bovine oocytes compared with GV oocytes (Leal et al, 2011). Our results point to INHBA expression being similar in CCs.…”

Section: Discussionsupporting

confidence: 54%

Relative transcript abundance in porcine cumulus cells collected from different sized follicles

Moros-Nicolás

Izquierdo-Rico

et al. 2020

Reprod Domestic Animals

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Crosstalk between the oocyte and surrounding cumulus cells (CCs) is essential for the production of competent oocytes. Previous studies have analysed the relative transcript abundance in oocytes derived from small (SF: <3 mm diameter)‐ and medium‐sized (MF: 3–6 mm diameter) follicles to determine the potential use of SF‐derived oocytes in assisted reproductive technologies (ART). The aim of this study was to examine the relative transcript abundance of CCs obtained from cumulus–oocyte complexes (COCs) derived from SF and MF. Nine genes were selected according to their importance for developmental competence: AT‐rich interaction domain 1B (ARID1B), bone morphogenic protein receptor 2 (BMPR2), CD44, follicle‐stimulating hormone receptor (FSHR), follistatin (FST), inhibin beta‐A (INHBA), luteinizing hormone receptor (LHR), nuclear receptor subfamily 2 group F member 6 (NR2F6) and vascular endothelial growth factor A (VEGFA). The expression of these genes was analysed by RT‐qPCR. The results pointed to significant differences in five genes, and the relative transcript abundance of SF‐derived CCs was lower in the case of INHBA, but higher in FSHR, FST, LHR and NR2F6 compared with MF‐derived CCs. We provide information of gene activity in the porcine CCs from different sized follicles, thus improving our understanding of oocyte biology and providing new markers that identify viable and competent oocytes.

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Section: Discussionsupporting

confidence: 54%

Relative transcript abundance in porcine cumulus cells collected from different sized follicles

Moros-Nicolás

Izquierdo-Rico

et al. 2020

Reprod Domestic Animals

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“…The average FPKM of the INHA gene in the ovary is 371, which is distributed in the peripheral region of the cytoplasm in the oocytes of large follicles and exists in the whole cytoplasm in the oocytes of small follicles; after in vitro fertilization, INHA is strongly expressed in the oocytes of small follicles, especially in the zona pellucida. Before and after in vitro fertilization, the INHB protein is highly expressed in the oocytes and cytoplasm of small follicles, and INHs can be used as a marker of porcine oocyte quality [27]. INHBA was involved in the synthesis of inhibin A/AB.…”

Section: Discussionmentioning

confidence: 99%

Integrated Multi-Tissue Transcriptome Profiling Characterizes the Genetic Basis and Biomarkers Affecting Reproduction in Sheep (Ovis aries)

Liu,

Fu,

et al. 2023

Genes

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The heritability of litter size in sheep is low and controlled by multiple genes, but the research on its related genes is not sufficient. Here, to explore the expression pattern of multi-tissue genes in Chinese native sheep, we selected 10 tissues of the three adult ewes with the highest estimated breeding value in the early study of the prolific Xinggao sheep population. The global gene expression analysis showed that the ovary, uterus, and hypothalamus expressed the most genes. Using the Uniform Manifold Approximation and Projection (UMAP) cluster analysis, these samples were clustered into eight clusters. The functional enrichment analysis showed that the genes expressed in the spleen, uterus, and ovary were significantly enriched in the Ataxia Telangiectasia Mutated Protein (ATM) signaling pathway, and most genes in the liver, spleen, and ovary were enriched in the immune response pathway. Moreover, we focus on the expression genes of the hypothalamic–pituitary–ovarian axis (HPO) and found that 11,016 genes were co-expressed in the three tissues, and different tissues have different functions, but the oxytocin signaling pathway was widely enriched. To further explore the differences in the expression genes (DEGs) of HPO in different sheep breeds, we downloaded the transcriptome data in the public data, and the analysis of DEGs (Xinggao sheep vs. Sunite sheep in Hypothalamus, Xinggao sheep vs. Sunite sheep in Pituitary, and Xinggao sheep vs. Suffolk sheep in Ovary) revealed the neuroactive ligand–receptor interactions. In addition, the gene subsets of the transcription factors (TFs) of DEGs were identified. The results suggest that 51 TF genes and the homeobox TF may play an important role in transcriptional variation across the HPO. Altogether, our study provided the first fundamental resource to investigate the physiological functions and regulation mechanisms in sheep. This important data contributes to improving our understanding of the reproductive biology of sheep and isolating effecting molecular markers that can be used for genetic selection in sheep.

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“…The next gene that showed a decrease in expression after IVM is inhibit subunit beta A (INHBA). It is believed that it plays an important role in reproduction and development through the coding of activins from TGF-beta superfamily [ 84 , 85 ] and is involved in the secretion of FSH from the pituitary [ 86 ], being also a marker of porcine oocyte quality [ 87 ]. A study on boars showed that INHBA is significant in testicular development, especially during puberty [ 88 ].…”

Section: Discussionmentioning

confidence: 99%

Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro

Kulus

Kranc

Ješeta

et al. 2020

Genes

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Polyspermia is an adverse phenomenon during mammalian fertilization when more than one sperm fuses with a single oocyte. The egg cell is prepared to prevent polyspermia by, among other ways, producing cortical granules (CGs), which are specialized intracellular structures containing enzymes that aim to harden the zona pellucida and block the fusion of subsequent sperm. This work focused on exploring the expression profile of genes that may be associated with cortical reactions, and evaluated the distribution of CGs in immature oocytes and the peripheral density of CGs in mature oocytes. Oocytes were isolated and then processed for in vitro maturation (IVM). Transcriptomic analysis of genes belonging to five ontological groups has been conducted. Six genes showed increased expression after IVM (ARHGEF2, MAP1B, CXCL12, FN1, DAB2, and SOX9), while the majority of genes decreased expression after IVM. Using CG distribution analysis in immature oocytes, movement towards the cortical zone of the oocyte during meiotic competence acquisition was observed. CGs peripheral density decreased with the rise in meiotic competence during the IVM process. The current results reveal important new insights into the in vitro maturation of oocytes. Our results may serve as a basis for further studies to investigate the cortical reaction of oocytes.

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Expression and Cellular Distribution of INHA and INHB before and afterIn VitroCultivation of Porcine Oocytes Isolated from Follicles of Different Size

Cited by 10 publications

References 33 publications

Relative transcript abundance in porcine cumulus cells collected from different sized follicles

Relative transcript abundance in porcine cumulus cells collected from different sized follicles

Integrated Multi-Tissue Transcriptome Profiling Characterizes the Genetic Basis and Biomarkers Affecting Reproduction in Sheep (Ovis aries)

Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro

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