Expression and changes of HSP70 in the rat forebrain subjected to gamma knife (100Gy) irradiation targeted on the caudate putamen and survived for different times

Rao, Zhi-Ren; Ge, Xu; Qiou, Jian-Yong; Yang, Tao; Duan, Li; Gong, Jianping

doi:10.1016/s0168-0102(00)00134-6

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…Thus, the observed histological changes can be considered to be the effects of irradiation during GKS. There are also some reports on irradiation-induced changes in heat shock protein 70, glial cell line derived neurotrophic factor, and certain metabolites in the rat brain. To the best of our knowledge, no comprehensive studies are available on unraveling the molecular changes elicited by irradiation during GKS.…”

Section: Introductionmentioning

confidence: 99%

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Gel-Based Proteomics of Unilateral Irradiated Striatum after Gamma Knife Surgery

et al. 2007

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Gamma knife surgery (GKS) is used for the treatment of various brain disorders. The biological effects of focal gamma ray irradiation on targeted or surrounding areas in the brain are not well-known. In the present study, we evaluated protein expression changes in the unilateral irradiated (60 Gy) striatum in rat. Striata of irradiated and control brains were dissected 16 h post-irradiation for analysis by large-format two-dimensional gel electrophoresis (2-DGE). In parallel, we also examined the un-targeted contralateral striatum over the control for potential changes in proteins patterns that may have occurred due to the effects of irradiation to the unilateral striatum. A total of 17 reproducible and differentially expressed silver nitrate-stained protein spots in the irradiated striatum was detected on 2-D gel. Their subsequent analysis by tandem mass spectrometry (nESI-LC-MS/MS) resulted in the identification of 13 nonredundant proteins. Interestingly, out of these 13 changed proteins, 2 proteins were also detected in the contralateral striatum. Some of the significantly changed proteins identified were creatine kinase, protein disulfide isomerase A3 precursor (PDA3), and peroxiredoxin 2 (Prx2). Western analysis with anti-PDA3 and anti-Prx2 antibodies revealed 4 and 2 cross-reacting protein spots on 2-D gel blots. Interestingly, after GKS, in the irradiated and un-irradiated striata, these spots showed a shift toward the acidic side, suggesting post-translational modifications. Taken together, these results indicate that unilateral irradiation during GKS triggers molecular changes in the bilateral striata.

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Section: Introductionmentioning

confidence: 99%

“…9 Thus, the observed histological changes can be considered to be the effects of irradiation during GKS. There are also some reports on irradiation-induced changes in heat shock protein 70, 10 glial cell line derived neurotrophic factor, 11 and certain metabolites 12 in the rat brain.…”

Section: Introductionmentioning

confidence: 99%

Gel-Based Proteomics of Unilateral Irradiated Striatum after Gamma Knife Surgery

et al. 2007

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“…The heat shock proteins (HSPs) are a group of stress proteins whose synthesis of some can be inducted and are suggested to play a role in neuron protection. The group of Rao has shown that from 3 hours to 30 days after GKRS, the expression of HSP70 changed in function of time and according to the cell types (Rao et al, 2000). This animal model was useful to perform assays Experimental Investigations in Small Animal Models 169 at protein and metabolite level in brain tissue after GKRS.…”

Section: Response Of Healthy Brain To Focused Radiationmentioning

confidence: 98%

Applications of Gamma Knife Radiosurgery for Experimental Investigations in Small Animal Models

Charest¹,

Paquette²,

Mathieu³

2011

Gamma Knife Radiosurgery

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“…How normal brain tissue responds to focally restricted irradiation, as occurs within and around tumor regions during targeted RT, and whether/how unirradiated or minimally irradiated tissue responds to radiation that is targeted to adjacent or distant areas, are less clear. Several laboratories have reported using the GK device for focal irradiation of the rat brain, targeting one hemisphere and comparing cellular and molecular changes in the targeted versus the “un-irradiated” hemisphere [24] [25] [26] [27], and/or reporting changes outside the targeted region [28] [29]. Significantly, however, even with the relatively steep gradient provided by the GK, the high target doses (60–100 Gy) used in these studies expose most or all of the brain to several Gy; they are similar to WBI with a high dose SRS “boost” to a target region.…”

Section: Introductionmentioning

confidence: 99%

Neurobiological responses to stereotactic focal irradiation of the adult rodent hippocampus

Schindler

Bourland

Forbes

et al. 2011

Journal of the Neurological Sciences

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Radiation effectively treats brain tumors and other pathologies but dose and treatment plans are limited by normal tissue injury, a major cause of morbidity in survivors. Clinically significant normal tissue injury can occur even with therapies that target pathological tissue and limit out-of-target irradiation. Elucidating the mechanisms underlying normal tissue injury is facilitated by studying the effects of focal irradiation and comparing irradiated and un-irradiated tissue in experimental animals. Young adult rats were irradiated using the Leksell Gamma Knife® with a 10 Gy maximum dose directed at the left hippocampus and shaped to minimize irradiation contralaterally. At least 95% of targeted hippocampus received ≥ 3 Gy, while all points in the contralateral hippocampus received < 0.3 Gy. Neuronal and microglial markers of damage were assessed in the targeted and contralateral hemispheres of Gamma Knife®-treated rats and compared to non-irradiated controls. Acute cell death and sustained changes in neurogenesis and in microglia occurred in the dentate gyrus of the targeted, but not the contralateral, hippocampus, providing experimental evidence that focal irradiation at doses received by peri-target regions during targeted radiation therapy produces robust normal tissue responses. Additional studies using this approach will facilitate assessment of in vivo dose responses and the cellular and molecular mechanisms of radiation-induced brain injury.

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Expression and changes of HSP70 in the rat forebrain subjected to gamma knife (100Gy) irradiation targeted on the caudate putamen and survived for different times

Cited by 8 publications

References 24 publications

Gel-Based Proteomics of Unilateral Irradiated Striatum after Gamma Knife Surgery

Gel-Based Proteomics of Unilateral Irradiated Striatum after Gamma Knife Surgery

Applications of Gamma Knife Radiosurgery for Experimental Investigations in Small Animal Models

Neurobiological responses to stereotactic focal irradiation of the adult rodent hippocampus

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