Expression and characterization of PhzE from P. aeruginosa PAO1: aminodeoxyisochorismate synthase involved in pyocyanin and phenazine-1-carboxylate production

Culbertson, Justin E.; Toney, Michael D.

doi:10.1016/j.bbapap.2012.10.010

Cited by 25 publications

(19 citation statements)

References 30 publications

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“…Pau21 is a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase responsible for condensation of phosphoenolpyruvate and erythrose 4-phosphate to 2-keto-3-deoxy-D-arabinoheptulosonate-7-phosphate, the first intermediate of the shikimate pathway [ 32 ], which is then converted to chorismate by a number of enzymes from the primary metabolic pathway. Chorismate is the branch point of the shikimate pathway, with one branch leading to phenazines through 2- a mino-2- d eoxy i so c horismate (ADIC) and trans -2,3- d i h ydro-3- h ydroxyanthranilic a cid (DHHA) by ADIC synthase and isochorismatase [ 33 , 34 ]. The early steps of the paulomycin ring A biosynthesis should follow an analogous route that includes a same set of transformations to generate DHHA by Pau18 (ADIC synthase) and Pau19 (isochorismatase).…”

Section: Resultsmentioning

confidence: 99%

Identification and Analysis of the Paulomycin Biosynthetic Gene Cluster and Titer Improvement of the Paulomycins in Streptomyces paulus NRRL 8115

Xie

Wang

et al. 2015

PLoS ONE

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The paulomycins are a group of glycosylated compounds featuring a unique paulic acid moiety. To locate their biosynthetic gene clusters, the genomes of two paulomycin producers, Streptomyces paulus NRRL 8115 and Streptomyces sp. YN86, were sequenced. The paulomycin biosynthetic gene clusters were defined by comparative analyses of the two genomes together with the genome of the third paulomycin producer Streptomyces albus J1074. Subsequently, the identity of the paulomycin biosynthetic gene cluster was confirmed by inactivation of two genes involved in biosynthesis of the paulomycose branched chain (pau11) and the ring A moiety (pau18) in Streptomyces paulus NRRL 8115. After determining the gene cluster boundaries, a convergent biosynthetic model was proposed for paulomycin based on the deduced functions of the pau genes. Finally, a paulomycin high-producing strain was constructed by expressing an activator-encoding gene (pau13) in S. paulus, setting the stage for future investigations.

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Section: Resultsmentioning

confidence: 99%

Identification and Analysis of the Paulomycin Biosynthetic Gene Cluster and Titer Improvement of the Paulomycins in Streptomyces paulus NRRL 8115

Xie

Wang

et al. 2015

PLoS ONE

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“…The strain KNU17Pc1 was subjected to PCR targeting the phenazine (phzE) gene, which is involved in antifungal activity [8,25]. The pair of primers and PCR conditions for phzE were performed as previously described [26] and a Zymoclean Gel DNA Recovery Kit (Zymo Research) was used to gel purify the PCR products.…”

Section: Detection Of Phze Genementioning

confidence: 99%

“…In this study, we tested if our strain KNU17Pc1 carries phzE, which catalyzes the first step in the biosynthesis of PCA and other phenazines [24,25]. The results of PCR using specific primers revealed the gene that codes for phzE (phenazine) was successfully amplified from strain KNU17Pc1 with the expected band size of 450 bp (Fig.…”

Section: Detection Of Phenazine Biosynthesis (Phze) Gene By Pcr Amplimentioning

confidence: 99%

Phenazine and 1-Undecene Producing Pseudomonas chlororaphis subsp. aurantiaca Strain KNU17Pc1 for Growth Promotion and Disease Suppression in Korean Maize Cultivars

Tagele

Lee

Kim

et al. 2019

Journal of Microbiology and Biotechnology

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In this study, strain KNU17Pc1 was tested for its antifungal activity against Rhizoctonia solani AG-1(IA), which causes banded leaf and sheath blight (BLSB) of maize. KNU17Pc1 was tested further for its broad-spectrum antifungal activity and in vitro plant growth promoting (PGP) traits. In addition, the in vivo effects of KNU17Pc1 on reduction of BLSB severity and seedling growth promotion of two maize cultivars under greenhouse conditions were investigated. On the basis of multilocus sequence analysis (MLSA), KNU17Pc1 was confirmed as P. chlororaphis subsp. aurantiaca. The study revealed that KNU17Pc1 had strong in vitro antifungal activity and was effective toward all in vitro PGP traits except phosphate solubilization. In this study, for the first time, a strain of P. chlororaphis against Colletotrichum dematium, Colletotrichum gloeosporioides, Fusarium oxysporum f.sp. melonis, Fusarium subglutinans and Stemphylium lycopersici has been reported. Further biochemical studies showed that KNU17Pc1 was able to produce both types of phenazine derivatives, PCA and 2-OH-PCA. In addition, solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis identified 13 volatile organic compounds (VOCs) in the TSB culture of KNU17Pc1, 1-undecene being the most abundant volatile. Moreover, for the first time, Octamethylcyclotetrasiloxan (D4), dimethyl disulfide, 2-methyl-1,3-butadiene and 1-undecene were detected in P. chlororaphis. Furthermore, this study reported for the first time the effectiveness of P. chlororaphis to control BLSB of maize. Hence, further studies are necessary to test the effectiveness of KNU17Pc1 under different environmental conditions so that it can be exploited further for biocontrol and plant growth promotion.

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“…As enzymes catalyzing the biosynthesis of PCN, the upregulation of proteins PhzA/B, PhzD, PhzF, PhzG and PhzH was line with the efficient PCN production in P3. PhzE is a anthranilate synthase that catalyzes the first step in the biosynthesis of phenazine and is responsible for converting chorismate, the end product of the shikimate pathway, and ammonia supplied by L-glutamine, into 2-amino-4-deoxychorismic acid (ADIC)22627. It is crucial for enhancement of PCN production of mutant P3.…”

Section: Disscusionmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis reveals potential factors associated with the enhancement of phenazine-1-carboxamide production in Pseudomonas chlororaphis P3

Jin

Peng

et al. 2016

Sci Rep

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Phenazine-1-carboxamide (PCN), a phenazine derivative, is strongly antagonistic to fungal phytopathogens. Pseudomonas chlororaphis HT66 is a PCN-producing, non-pathogenic biocontrol strain, and we obtained the mutant P. chlororaphis P3, which produces 4.7 times more PCN than the wild-type HT66 strain. To reveal the cause of PCN production enhancement in P3 and find potential factors related to PCN biosynthesis, an iTRAQ-based quantitative proteomic analysis was used to study the expression changes between the two strains. Of the 452 differentially expressed proteins, most were functionally mapped into PCN biosynthesis pathway or other related metabolisms. The upregulation of proteins, including PhzA/B, PhzD, PhzF, PhzG, and PhzH, involved in PCN biosynthesis was in agreement with the efficient production of PCN in P3. A number of proteins that function primarily in energy production, amino acid metabolism, and secondary metabolism played important roles in PCN biosynthesis. Notably, proteins involved in the uptake and conversion of phosphate, inorganic nitrogen sources, and iron improved the PCN production. Furthermore, the type VI secretion system may participate in the secretion or/and indirect biosynthetic regulation of PCN in P. chlororaphis. This study provides valuable clues to better understand the biosynthesis, excretion and regulation of PCN in Pseudomonas and also provides potential gene targets for further engineering high-yield strains.

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Expression and characterization of PhzE from P. aeruginosa PAO1: aminodeoxyisochorismate synthase involved in pyocyanin and phenazine-1-carboxylate production

Cited by 25 publications

References 30 publications

Identification and Analysis of the Paulomycin Biosynthetic Gene Cluster and Titer Improvement of the Paulomycins in Streptomyces paulus NRRL 8115

Identification and Analysis of the Paulomycin Biosynthetic Gene Cluster and Titer Improvement of the Paulomycins in Streptomyces paulus NRRL 8115

Phenazine and 1-Undecene Producing Pseudomonas chlororaphis subsp. aurantiaca Strain KNU17Pc1 for Growth Promotion and Disease Suppression in Korean Maize Cultivars

iTRAQ-based quantitative proteomic analysis reveals potential factors associated with the enhancement of phenazine-1-carboxamide production in Pseudomonas chlororaphis P3

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