Expression and characterization of the chitinases from Serratia marcescens GEI strain for the control of Varroa destructor, a honey bee parasite

Potential of Chitinolytic Bacillus amyloliquefaciens SAHA 12.07 and Serratia marcescens KAHN 15.12 as Biocontrol Agents of Ganoderma boninense

“…The similar result also reported that chitinase of B. amyloliquefaciens V656 had optimum activity at pH 6 and 7 (Wang et al, 2002). Similarly, chitinase of S. marcescens GEI and Serratia marcescens MO-1 had optimum activity at pH 7 (Tu et al, 2010;Okay et al, 2013). Chitinase of bacteria generally have a pH range from acidic to alkaline.…”

“…Chitinase activity at temperature 20°C was only found on S. marcescens KAHN 15.12 although, it was lower than the optimum temperature. The same result also reported that chitinase had optimum activity between 35-75°C (Wang et al, 2002;Dai et al, 2011;Tu et al, 2010;Zarei et al, 2011;Asril et al, 2014;Laribi-Habchi et al, 2015).…”

“…Microbiol., 10 (10): 452-465, 2015 expansum (Kim and Chung, 2004;Abdullah et al, 2008;Arrebola et al, 2010;Hu et al, 2010). Serratia marcescens was also reported as a potential biocontrol agent on Pythium ultimum, Pyricularia oryzae and Varroa destructor (Jaiganesh et al, 2007;Roberts et al, 2007;Tu et al, 2010). Microbial which have a broad spectrum of inhibition pathogenic are very well be used as biocontrol agents (Wang et al, 2014).…”

“…This occurred because of S. marcescens KAHN 15.12 had a chitinase specific activity that was higher than B. amyloliquefaciens SAHA 12.07, it was alleged the ability to lyse G. boninense cell wall will be higher and NAG will be produced in the higher number. This is supported that Serratia marcescens has also known as a potential chitinase producing bacteria that has been widely applied as biocontrol (Tu et al, 2010;Zarei et al, 2011;Okay et al, 2013;Fadhil et al, 2014). In the condition above optimum time, here concentration of NAG decreased.…”

“…The content of chitin in the mycelium of the phylum Basidiomycota approximately 26.4-64.5% (Di Mario et al, 2008), whereas in Ganoderma lucidum is 41% (Alvarez et al, 2014). Chitin can be degraded enzymatically by chitinase which function as biocontrol in fungal pathogens (Singh et al, 1999;Dai et al, 2011) and insect pathogens Nurdebyandaru et al, 2010;Tu et al, 2010;Lee and Kim, 2015).…”