Expression and Function of Growth Factor Ligands and Receptors in Preimplantation Mouse Embryos

Rappolee, Daniel A.; Sturm, Karin S.; Schultz, Gilbert A.; Basilico, Claudio; Bowen‐Pope, Daniel F.; Pedersen, Roger A.; Werb, Zena

doi:10.1007/978-1-4612-3162-2_15

Cited by 36 publications

(60 citation statements)

References 54 publications

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“…Subsequently, a number of studies have elucidated the ontogeny of insulin and IGF-I and -11 ligands and their cognate receptor expression by preimplantation mouse embryos. To summarize, mRNA of IGF-I is detectable in oocytes, cleavage stage, and blastocyst stage embryos (Doherty et al, 1994); IGF-I1 is expressed in two-cell and subsequent stage embryos (Rappolee et al, 1990); however, insulin is not detectable in any preimplantation stage mouse embryos. The IGF-I1 receptor is expressed from the two-cell stage onwards (Rappolee et al, 1990), while the expression of receptors for insulin and IGF-I is found first at the compacted eight-cell stage (Rappolee et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Mouse embryonic stem cells express receptors of the insulin family of growth factors

Shi

Dhir

Kesavan

et al. 1995

Molecular Reproduction Devel

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Insulin and insulin-like growth factors (IGF-I and -II) are members of a family of growth factors which are known to be developmentally regulated during preimplantation mouse embryogenesis. The physiological actions of the insulin family of growth factors are mediated by interactions with specific cell surface receptors that are detectable on the cells of preimplantation mouse embryos. Mouse embryonic stem (ES) cells are totipotent cells derived directly from the inner cell mass of the blastocyst. ES cells have the ability to differentiate into all three germ layers and have unlimited growth potential under certain culture conditions. The great advantage of ES cells is the ability to obtain large amounts of tissue for biochemical studies as compared with preimplantation embryos. To examine in greater detail the biological actions of the insulin family of growth factors, the expression of their cognate receptors on ES cells was examined. ES cells were cultured in DMEM medium supplemented with leukemia inhibitory factor (LIF) to maintain the undifferentiated state. Receptor expression was evaluated at the mRNA level using the reverse transcription polymerase chain reaction (RT-PCR), and at the protein level by radioactive labeled ligand-receptor binding assay. Using RT-PCR, mRNAs of all three growth factor receptors were detected in ES cells. Messenger RNA from ES cells was reverse transcribed into cDNA by AMV reverse transcriptase at 42 degrees C for 1 hr. The reverse transcription reaction was amplified with Taq polymerase and specific primers for insulin, IGF-I, or IGF-II receptors by PCR. RT-PCR and the control plasmid cDNA PCR products were resolved electrophoretically on 3% agarose gels. Each amplified PCR product showed the predicted correct size.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Introductionmentioning

confidence: 99%

Mouse embryonic stem cells express receptors of the insulin family of growth factors

Shi

Dhir

Kesavan

et al. 1995

Molecular Reproduction Devel

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“…The growth rate of embryos is increased by increasing culture density [1], and by adding exogenous growth factors, such as insulin, epidermal growth factor (EGF) and insulin-like growth factor-II (IGF-II) [1][2][3][4]. These growth factors and their cognate receptor tyrosine kinases are expressed during the preimplantation stage [5,6]. These reports suggest that signal tr an s d u c t i on s ys te m s, i n c l u d i n g t y r o si n e phosphorylation, are involved in growth regulation in embryos.…”

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confidence: 99%

Dynamic Changes in the Expression of Protein Tyrosine Phosphatases During Preimplantation Mouse Development: Semi-Quantification by Real-Time PCR

Hara

Sakuma

Sakai

et al. 2003

Journal of Reproduction and Development

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Abstract. Protein tyrosine phosphatase (PTP) expression was examined in preimplantation mouse embryos. We previously reported that SHP-2, LAR, PTPT9, SHP-1, and mRPTPB were expressed in preimplantation mouse embryos. Here, we examined changes in the expression levels of these PTPs during preimplantation development. cDNA was obtained by reverse transcription of embryo mRNA, amplified with 10 PCR cycles, and then subjected to real-time fluorescence-monitored PCR. Experiments with an mRNA dilution series revealed that the data obtained matched the quantities of mRNA used. The measurements obtained with real-time fluorescence-monitored PCR showed that the expression of each PTP mRNA changed dynamically, and that each had a different expression pattern. This suggests that PTPs are involved in the regulation of growth and differentiation during preimplantation development. Key words: Mouse embryo, Protein tyrosine phosphatase, Gene expression, Real-time PCR (J. Reprod. Dev. 49: [323][324][325][326][327][328] 2003) rotein tyrosine phosphorylation plays an important role in the regulation of various cellular functions, including growth, differentiation and tumor transformation. Reversible tyrosine phosphorylation is involved in signal transduction that is mediated by extracellular ligands, such as growth factors, hormones and cytokines, which have receptors or receptor-associated proteins containing protein tyrosine kinase (PTK) activity [1]. Several studies of growth factors suggest that tyrosine phosphorylation is involved in the d e v e l o p m e n t a l r e g u l a t i on o f m a m m a l i a n preimplantation embryos. The growth rate of embryos is increased by increasing culture density [1], and by adding exogenous growth factors, such as insulin, epidermal growth factor (EGF) and insulin-like growth factor-II (IGF-II) [1][2][3][4]. These growth factors and their cognate receptor tyrosine kinases are expressed during the preimplantation stage [5,6]. These reports suggest that signal tr an s d u c t i on s ys te m s, i n c l u d i n g t y r o si n e phosphorylation, are involved in growth regulation in embryos.Since cellular levels of tyrosine phosphorylation are regulated by the activity of PTKs and protein tyrosine phosphatases (PTPs), both should play important roles in regulating various cellular functions. To date, PTP expression has been reported in various tissues and cell lines, but the function of a considerable number of these PTPs remains unclear.Our previous report showed that SHP-2, LAR,

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“…Furthermore, IGF-I appears to act as a survival factor by decreasing apoptosis [7]. IGF-I receptors are present in mouse embryos from the compacted 8-cell stage of development and in rabbits from the morula stage onward [8][9][10]. However, studies on the synthesis of IGF-I by the conceptus prior to implantation have led to conflicting results.…”

Section: Introductionmentioning

confidence: 92%

“…However, studies on the synthesis of IGF-I by the conceptus prior to implantation have led to conflicting results. While some investigators were unable to detect IGF-I mRNA in preimplantation embryos [9,11,12], others described its presence from 1 This work was supported by the Deutsche Forschungsgemeinschaft (DFG-grant, He 2688/1-1, to A.H.) and the Thoroughbred Breeders' Association of Great Britain. [13,14].…”

Section: Introductionmentioning

confidence: 96%

Horse Conceptuses Secrete Insulin-Like Growth Factor-Binding Protein 31

Herrler

Pell

Allen

et al. 2000

Biology of Reproduction

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Insulin-like growth factor-I (IGF-I) promotes early embryonic development in several species. In the rabbit, IGF-I binds to the embryonic coats from Day 3 of development onward by a 38-kDa protein that is probably insulin-like growth factor-binding protein 3 (IGFBP3). In the present study, ligand, Western, and Northern blot analyses were used to demonstrate the presence of IGF-I-binding activity, several immunoreactive IGFBP3 proteins, and IGFBP3 mRNA in horse conceptuses with particularly large amounts of immunoreactive IGFBP3 in the conceptus capsule. In addition, immunoprecipitation of radiolabeled proteins showed that cultured horse conceptuses secreted IGFBP3 into the culture medium. Endometrial samples from mares also contained IGFBP3 mRNA and protein; but there was no evidence of secretion of IGFBP3 into the uterine lumen by ligand blot analysis, and there was evidence of only very small amounts by Western blot analysis. These results indicate that the horse conceptus secretes significant quantities of IGFBP3 toward the conceptus capsule from as early as Day 10 after ovulation. Thus, most of the IGFBP3 contained within the capsule, which binds IGF-I to this special extracellular matrix of the preimplantation horse conceptus, is likely to be embryonic in origin. IGFBP3 in the horse conceptus capsule may enhance or modulate the action of IGFs on the developing conceptus.

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Expression and Function of Growth Factor Ligands and Receptors in Preimplantation Mouse Embryos

Cited by 36 publications

References 54 publications

Mouse embryonic stem cells express receptors of the insulin family of growth factors

Mouse embryonic stem cells express receptors of the insulin family of growth factors

Dynamic Changes in the Expression of Protein Tyrosine Phosphatases During Preimplantation Mouse Development: Semi-Quantification by Real-Time PCR

Horse Conceptuses Secrete Insulin-Like Growth Factor-Binding Protein 31

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