Expression and function of long non‑coding RNA LINC01420 in thyroid cancer

Abstract

The Human Genome Project revealed that >90% of the human genome was found to transcribe non-coding RNAs, including micro RNAs and long non-coding RNAs (lncRNAs). lncRNAs have been identified to play a crucial role in cancer progression. Thyroid cancer (TC) is a common type of endocrine cancer; however, the functional roles of lncRNAs in TC have yet to be fully elucidated. The present study investigated whether LINC01420 was upregulated in TC tissues, compared with normal thyroid tissues, and the results suggested that LINC01420 may play a regulatory role in TC. Bioinformatics analysis demonstrated that LINC01420 was associated with translation, rRNA processing, mRNA splicing, regulation of transcription, DNA repair and double-strand break repair. Furthermore, the exact role of LINC01420 in TC was explored by performing a loss-of-function assay, which revealed that the knockdown of LINC01420 inhibited TC cell proliferation and cell cycle progression. The findings of the present study provide a novel insight into the molecular mechanisms underlying TC development. Moreover, they suggest that LINC01420 may serve as a potential therapeutic target for the treatment of TC, and that increased LINC01420 expression levels show potential as a prognostic marker for the disease.

LncRNA PVT1 Acts as a Tumor Promoter in Thyroid Cancer and Promotes Tumor Progression by Mediating miR-423-5p-PAK3

“…22 Their abnormal expression in cancer patients may cause the normal physiological process of cells to get out of control and eventually lead to the development and progression of tumors. 23 This study explored TC, which is a common malignant endocrine tumor, 24 so we analyzed the potential regulatory value of LncRNAs through clinical samples and cell functional experiments in vitro. PVT1 has been confirmed to function as a tumor promoter in TC, but its molecular mechanism remains unclear.…”

Emerging role of long noncoding RNA-encoded micropeptides in cancer

“…Linc01420 is upregulated in nasopharyngeal cancer and is positively associated with tumor metastasis and an unfavorable prognosis [59]. Linc01420 also facilitates cell proliferation and migration in melanoma [60], promotes pancreatic cancer development via targeting KRAS [61], and accelerates cell cycle progression and cell proliferation in thyroid cancer [62]. Proteomics analysis showed that the 7 kDa micropeptide, non-annotated P-body dissociating micropeptide (NoBody), was translated from LINC01420/LOC550643 in leukemia K562 and breast cancer MDA-MB-231 cells (Fig.…”

LOC550643, a Long Non-coding RNA, Acts as Novel Oncogene in Regulating Breast Cancer Growth and Metastasis

“…Furthermore, LOC550643 knockdown could suppress pancreatic cancer cell growth and invasion capability through releasing miR-494-3p sponging. Relevant studies have also investigated the role of LOC550643 in thyroid cancer, revealing significantly higher levels of LOC550643 expression in thyroid cancer cells and that LOC550643 knockdown inhibited cancer cell growth by impairing cell cycle progression ( Luo et al, 2020 ). By contrast, Konina et al (2019) reported an opposite role, in which LOC550643 inhibited melanoma cell migration ability but did not influence cell growth.…”