Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata

Li, Huie; Xu, Yan; Xiao, Yu; Zhu, Ziguo; Xie, Xiaoqing; Zhao, Heqing; Wang, Yuejin

doi:10.1007/s00425-010-1258-y

Cited by 110 publications

(72 citation statements)

References 49 publications

(69 reference statements)

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“…These genotypes were maintained in the grape germplasm resources orchard of Northwest A&F University, Yangling, China (34°200ꞌN, 108°240ꞌE). When shoots of the vines were 30-40 cm in length, the third to sixth fully expanded young leaves below the apex were selected for treatments (Li et al, 2010a). E. necator was collected from the highly susceptible V. adstricta Hance genotype 'Taishan-2' in the grape germplasm resource orchard, Northwest A&F University.…”

Section: Plant and Pathogen Materialsmentioning

confidence: 99%

“…At each inoculation, leaves were sampled at 0,6,12,24,48,72,96, and 120 h post-inoculation (hpi), immediately frozen in liquid nitrogen, and stored at -80°C. Selection and treatment of grapevine leaves with defense signaling molecules [SA, methyl jasmonate (MeJA), and ethephon (Eth)] were performed as described in Li et al, 2010a. The treated grapevine leaves were sampled at 0, 0.5, 1, 3, 6, and 12 h, immediately frozen in liquid nitrogen, and stored at -80°C.…”

Section: Biotic and Signaling Molecule Treatmentsmentioning

confidence: 99%

“…qRT-PCR and data analysis were conducted as described in Li et al (2010a). Firststrand cDNA was synthesized from 1 μg DNase-treated total RNA using PrimeScript TM RTase (TaKaRa Biotechnology).…”

Section: Vtc Expression Analysis By Qrt-pcr In Different Grapevine Gementioning

confidence: 99%

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Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

Hou

Li²,

Gao³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Using rapid amplification of cDNA ends, a full-length cDNA sequence of a GDP-L-galactose phosphorylase-like gene was isolated from leaves infected by Erysiphe necator in the Chinese wild (Vitis pseudoreticulata) clone, 'Baihe-35-1', an E. necator-resistant genotype. The full-length cDNA, designated as VpVTC, comprised 1943 bp and putatively encodes a 453-amino acid polypeptide containing an HIT motif. The deduced amino acid sequence showed high similarity with that of VTC genes from other plants. The expression of VpVTC, determined by reverse transcriptase-polymerase chain reaction, was induced by E. necator and defense signaling molecules, including salicylic acid, methyl jasmonate, and ethephon, in 'Baihe-35-1', the V. quinquangularis genotype 'Shang-24', and the E. necator-susceptible A GGP-like gene from a Chinese wild Vitis species V. pseudoreticulata genotype, 'Hunan-1'. Transcript levels of VpVTC correlated well with the degree of disease resistance in the 3 genotypes. Maximum induction of VpVTC by E. necator (>7-fold at 96 h postinoculation) occurred in 'Baihe-35-1', which also showed the fastest response to signaling molecules. Upregulating the expression of VpVTC in 'Baihe-35-1' resulted in a gradual increase in the ascorbic acid concentration of leaves inoculated with E. necator. Furthermore, VpVTC was expressed in leaves, stems, inflorescence, tendrils, and fruit at all developmental stages, with the highest level occurring in fruit 35 days after flowering.

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Section: Plant and Pathogen Materialsmentioning

confidence: 99%

Section: Biotic and Signaling Molecule Treatmentsmentioning

confidence: 99%

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Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

Hou

Li²,

Gao³

et al. 2013

Genet. Mol. Res.

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“…Recent studies also characterized several WRKY genes in cultivated grapevines and Chinese wild Vitis pseudoreticulata. All of them have been shown to regulate biotic or abiotic responses [20][21][22][23][24][25][26], but there has been no report about grapevine WRKY transcription factors affecting the ABA signaling and ABA production, until now.…”

Section: Introductionmentioning

confidence: 99%

VvWRKY13 enhances ABA biosynthesis in Vitis vinifera

Hao

Hou

et al. 2017

Acta Soc Bot Pol

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Abscisic acid (ABA) plays critical roles in plant growth and development as well as in plants' responses to abiotic stresses. We previously isolated VvWRKY13, a novel transcription factor, from Vitis vinifera (grapevine), and here we present evidence that VvWRKY13 may regulate ABA biosynthesis in plants. When VvWRKY13 was ectopically expressed in Arabidopsis, the transgenic lines showed delayed seed germination, smaller stomatal aperture size, and several other phenotypic changes, indicating elevated ABA levels in these plants. Sequence analysis of several genes that are involved in grapevine ABA synthetic pathway identified WRKY-specific binding elements (W-box or W-like box) in the promoter regions. Indeed, transient overexpression of VvWRKY13 in grapevine leaves significantly increased the transcript levels of ABA synthetic pathway genes. Taken together, we conclude that VvWRKY13 may promote ABA production by activating genes in the ABA synthetic pathway.

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“…A large number of WRKY genes have been identified in plants (Mangelsen et al 2008;Liu & Ekramoddoullah 2009;Pandey & Somssich 2009;Rushton et al 2010;. They are involved in multiple biotic and abiotic stress responses, and developmental and physiological processes, such as anthocyanin biosynthesis, senescence, trichome development, starch biosynthesis, and hormone responses (Lagace & Matton 2004;Jiang & Yu 2009;Wu et al 2009;Chen et al 2010;Li et al 2010;Miao & Zentgraf 2010;Ren et al 2010;Liu et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco

Pan

Ying

et al. 2014

Biologia

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Dendrobium seedlings showed low survival rate when they were transferred from in vitro conditions to greenhouse or field environment. One of the major reasons is their low tolerance to environmental changes. WRKY transcription factors are one of the largest families of transcriptional regulators in plants. They are involved in various biotic and abiotic stress responses. One DnWRKY11 gene was isolated from Dendrobium nobile. To explore the function of DnWRKY11 in Dendrobium defense responses to abiotic stress, it was overexpressed in tobacco. Under salt and drought stresses, the DnWRKY11 transgenic tobacco showed higher germination rate, longer root length, higher fresh weight, higher activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and lower content of malonidialdehyde (MDA) than the wild type. These results proved the important roles of DnWRKY11 in plant response to drought and salt stresses, and provided a potential gene for improving environmental stress tolerance of Dendrobium seedlings.

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Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata

Cited by 110 publications

References 49 publications

Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

VvWRKY13 enhances ABA biosynthesis in Vitis vinifera

Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco

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