Expression and functional characterization of three β-defensins in grass carp (Ctenopharyngodon idella)

Feng, Jianhua; Zhao, Jia; Yuan, Gaoliang; Zhu, Xiao-Zhen; Liu, Qin; Wu, Kaizheng; Wang, Junya; Zou, Jun

doi:10.1016/j.dci.2022.104616

Cited by 8 publications

(4 citation statements)

References 55 publications

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“…A. hydrophila were prepared as previously described [ 35 ], and were resuspended in sterile PBS at 1 × 10 7 CFU/mL. Thirty grass carp (15 ± 2 g) were randomly placed into two tanks, each containing 15 fish.…”

Section: Methodsmentioning

confidence: 99%

“…Heat-inactivated A. hydrophila were prepared as previously described [ 35 ]. In brief, when OD 600 of A. hydrophila culture reached 0.6, 1 mL of bacteria were collected and washed three times with sterile PBS.…”

Section: Methodsmentioning

confidence: 99%

“…2.5. Expression Analysis of il21 in the Primary HKLs after Stimulation with Inactivated A. hydrophila and LPS Heat-inactivated A. hydrophila were prepared as previously described [35]. In brief, when OD 600 of A. hydrophila culture reached 0.6, 1 mL of bacteria were collected and washed three times with sterile PBS.…”

Section: Hematoxylin and Eosin (Hande) Staining Of Grass Carp Intestinementioning

confidence: 99%

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The Biological Functions and Intestinal Inflammation Regulation of IL-21 in Grass Carp (Ctenopharyngodon idella) during Infection with Aeromonas hydrophila

Yuan,

Zhao,

Zhang

et al. 2023

Cells

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Interleukin (IL) 21 is a pleiotropic cytokine that plays an important role in regulating innate and adaptive immune responses. In fish, the biological functions and cell source of IL-21 remain largely unknown. In this study, we performed qRT-PCR, Western blotting and immunofluorescent microscopy to examine the expression of IL-21 at the mRNA and protein levels. We found that il21 expression was induced in the primary head kidney leukocytes of grass carp (Ctenopharyngodon idella) by heat-inactivated Aeromonas hydrophila (A. hydrophila) and LPS and in tissues after infection with A. hydrophila. Recombinant IL-21 protein produced in the CHO-S cells was effective in elevating the expression of antibacterial genes, including β-defensin and lysozyme, and, interestingly, inhibited the NF-κB signaling pathway. Furthermore, we investigated the response of the IL-21 expressing cells to A. hydrophila infection. Immunofluorescent assay showed that IL-21 protein was detected in the CD3γ/δ T cells and was markedly accumulated in the anterior, middle and posterior intestine. Collectively, the results indicate that IL-21 plays an important role in regulating the intestinal inflammation induced by bacterial infection in grass carp.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Hematoxylin and Eosin (Hande) Staining Of Grass Carp Intestinementioning

confidence: 99%

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The Biological Functions and Intestinal Inflammation Regulation of IL-21 in Grass Carp (Ctenopharyngodon idella) during Infection with Aeromonas hydrophila

Yuan,

Zhao,

Zhang

et al. 2023

Cells

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“…In addition, the expression of Citl1a in the hindgut, gills and head kidney of grass carp upon A. hydrophila stimulation was analyzed. A. hydrophila was provided by the National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, and the challenge experiment was conducted as previously described [21] . In brief, a single colony of A. hydrophila was inoculated in the LB medium and cultured with constant shaking (at 170 rpm) overnight at 28 °C.…”

Section: Methodsmentioning

confidence: 99%