Expression and Functional Characterization of the Serine Protease Inhibitor Neuroserpin in Endocrine Cells

Hill, Rena M.; Coates, Leigh C.; Parmar, Parmjeet K.; Mezey, Éva; Pearson, John; Birch, Nigel P.

doi:10.1111/j.1749-6632.2002.tb04503.x

Cited by 14 publications

(11 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neuroserpin may alter cell survival and intercellular communication in mature endocrine tissues by regulating cell adhesion. 23 These findings support the hypothesis that neuroserpin may also cause an outgrowth response of prostate cancer cells and promote the migration of cancer cells.…”

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

Neuroserpin (PI‐12) is upregulated in high‐grade prostate cancer and is associated with survival

Hasumi

Ishiguro

Nakamura

et al. 2005

Intl Journal of Cancer

View full text Add to dashboard Cite

We carried out Genechip analysis using prostate cancer and nonmalignant tissue to identify specific genes related to prostate cancer. We focused on neuroserpin (PI-12), which has been identified as one of the genes with high expression in prostate cancer. We analyzed the relationship between its expression pattern and clinical characteristics. Prostate cancer and normal prostate tissue were analyzed by Affymetrix GeneChip technology. We carried out real-time quantitative PCR on a total of 102 specimens: 45 of normal prostate, 45 of previously untreated prostate cancer (constituting 45 pairs of samples obtained at radical prostatectomy, with each pair dissected from the same prostate specimen) and 12 of recurrent hormone refractory prostate cancer (HRPC). Results showed that the neuroserpin gene was more highly expressed in prostate cancer than in normal prostate tissue. Neuroserpin expression in untreated prostate cancer was significantly higher than that in normal prostate. In HRPC it was significantly higher than that in untreated prostate cancer and normal prostate. In untreated prostate cancer, neuroserpin expression was significantly higher in high grade tumors such as poorly differentiated adenocarcinoma than in lower grade tumors such as well or moderately differentiated adenocarcinoma. Higher neuroserpin expression was associated with shorter recurrence-free survival after radical prostatectomy, shorter recurrence-free survival in HRPC patients and shorter overall survival in HRPC patients. The neuroserpin gene may be associated with the development, progression and aggressiveness of prostate cancer. Our present data suggests that higher neuroserpin expression may predict an unfavorable outcome after radical prostatectomy or hormone therapy. ' 2005 Wiley-Liss, Inc.

show abstract

Section: Discussionsupporting

confidence: 79%

“…23 The extension of cellular processes by endocrine cells may aid in the migration of cells to their final location in the developing gland. Neuroserpin may alter cell survival and intercellular communication in mature endocrine tissues by regulating cell adhesion.…”

Section: Discussionmentioning

confidence: 99%

Neuroserpin (PI‐12) is upregulated in high‐grade prostate cancer and is associated with survival

Hasumi

Ishiguro

Nakamura

et al. 2005

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…There is a growing body of evidence that serine proteases play an essential role in hormone biosynthesis in endocrine organs (Hill et al 2002). Our data show that expression of several serine protease inhibitors in adrenals is suppressed by LXR agonist treatment including Spil-1, -3 and -4 (Figs 5 and 6).…”

Section: Discussionmentioning

confidence: 52%

Genome-wide expression profiling; a panel of mouse tissues discloses novel biological functions of liver X receptors in adrenals

Steffensen¹,

Neo²,

Stulnig³

et al. 2004

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

The liver X receptors and (LXR and LXR ) are members of the nuclear receptor superfamily of proteins which are highly expressed in metabolically active tissues. They regulate gene expression of critical genes involved in cholesterol catabolism and transport, lipid and triglyceride biosynthesis and carbohydrate metabolism in response to distinct oxysterols and intermediates in the cholesterol metabolic pathway. The biological roles of the LXRs in tissues other than liver, intestine and adipose tissue are poorly elucidated. In this study we used global gene-expression profiling analysis to detect differences in expression patterns in several tissues from mice fed an LXR agonist or vehicle. Our results show that LXR plays an important role in the kidney, lung, adrenals, brain, testis and heart where several putative LXR target genes were found. The effects of the LXRs were further analysed in adrenals where treatment with an LXR agonist induced expression of adrenocorticotrophic hormone receptor, suppressed expression of uncoupling protein (UCP)-1 and UCP-3 as well as several glycolytic enzymes and led to increased serum corticosterone levels. These results indicate novel biological roles of the LXR including regulation of energy metabolism, glycolysis and steroidogenesis in the adrenals via alteration of expression profiles of putative target genes.

show abstract

“…Also in PC12 and AtT20 cell lines transfected with neuroserpin, the protein was localized in secretory granules [31]. Proposed functions of neuroserpin in the pituitary include the regulation of intragranular proteases and, following regulated secretion, inhibition of enzymes regulating cell-extracellular matrix interactions [29]. On the basis of results from studies on Drosophila Spn4.1, Osterwalder et al [18] also suggested that neuroserpin, the closest vertebrate Spn4.1 orthologue, might function intracellularly as a regulator of prohormone processing.…”

Section: Discussionmentioning

confidence: 99%

“…Neuroserpin represents a recently identified serpin family member, which is expressed primarily in neuronal and neuroendocrine tissues. Thus far, several roles for neuroserpin have been proposed, among which an involvement in neuronal plasticity and learning [10], neuronal cell survival [12], neurite outgrowth, and intercellular communication in mature neuroendocrine tissues by the regulation of cell adhesion [29]. …”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis and Expression of Neuroserpin in <i>Xenopus laevis</i>

Groot

Pol²,

Martens³

2005

Neuroendocrinology

View full text Add to dashboard Cite

Serine protease inhibitors form a diverse family of proteins of which most members inhibit target serine proteases. Neuroserpin is a member of this family. Here, we have characterized neuroserpin in the nonmammalian species Xenopus laevis and found a high degree of aminoacid sequence conservation, especially of the reactive center loop, of the Xenopus protein compared to mammalian and chicken neuroserpin sequences, suggesting a conserved target specificity. Neuroserpin mRNA and protein were expressed throughout Xenopus development, while in the adult frog high mRNA expression was found in neuronal and neuroendocrine tissues, and the reproductive organs, and the neuroserpin protein was detected mainly in brain and pituitary. More specifically, in Xenopus pituitary neuroserpin mRNA was expressed higher in the neurointermediate lobe than in the pars distalis. At the protein level, we detected a 55-kDa neuroserpin protein in the pars nervosa, two neuroserpin proteins of 44- and 50-kDa in the melanotrope cells of the pars intermedia, and a 46-kDa product in the pars distalis. On the basis of its relatively high degree of sequence conservation and its expression pattern, we conclude that Xenopus neuroserpin may play an important physiological role, e.g. as a serine protease inhibitor during development, and for proper neuronal and neuroendocrine cell functioning.

show abstract

Expression and Functional Characterization of the Serine Protease Inhibitor Neuroserpin in Endocrine Cells

Cited by 14 publications

References 53 publications

Neuroserpin (PI‐12) is upregulated in high‐grade prostate cancer and is associated with survival

Neuroserpin (PI‐12) is upregulated in high‐grade prostate cancer and is associated with survival

Genome-wide expression profiling; a panel of mouse tissues discloses novel biological functions of liver X receptors in adrenals

Comparative Analysis and Expression of Neuroserpin in <i>Xenopus laevis</i>

Contact Info

Product

Resources

About