Expression and localization of bone morphogenetic protein6 (BMP6) in the corpus luteum during different stages of estrous cycle in the buffalo (Bubalus bubalis)

Abstract

Emerging evidence suggests that Bone Morphogenetic Proteins (BMPs), which belong to the Transforming Growth Factor-β (TGF-β) super-family, are known to be involved in the follicular growth and steroid production in different species. This study describes BMP6 bearing in corpus luteum over various stages of estrous cycle. The bubaline CL was classified into four stages according to the morphology and progesterone (P4) concentration. The real time PCR and immunoblot studies revealed that BMP6 was significantly (P<0.05) unregulated during the mid stage of CL that was consistent with immunohistochemical localization in the luteal cells. The transcriptional and translational expressions of BMP6 in the early and late CL were comparable and significantly (P<0.05) lower than that of mid CL. In conclusion, BMP6 expression is dependent on the stage of CL in the buffalo.