Expression and localization of ghrelin and its receptor in ovarian follicles during different stages of development and the modulatory effect of ghrelin on granulosa cells function in buffalo

Gupta, Mahesh; Dangi, S.S.; Singh, Gyanendra; Sarkar, Mihir

doi:10.1016/j.ygcen.2014.09.013

Cited by 15 publications

(13 citation statements)

References 39 publications

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“…Our findings are in accordance with previous studies that intense GHSR-1a immunoreactivity was noted in the granulosa cells of developing follicles [16,46], suggesting an important role for ghrelin and GHSR-1a in the ovary [47]. Some previous studies have indicated that ghrelin has an anti-inflammation effect.…”

Section: Discussionsupporting

confidence: 93%

Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells

Chao

Sun

Peng

et al. 2016

IJMS

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Cyclooxygenase-2 (COX-2) and interleukin-8 (IL-8) are two important inflammatory mediators in ovulation. Ghrelin may modulate inflammatory signaling via growth hormone secretagogue receptors. We investigated the role of ghrelin in KGN human ovarian granulosa cells using protein kinase C (PKC) activator phorbol 12, 13-didecanoate (PDD) and synthetic ghrelin analog growth hormone releasing peptide-2 (GHRP-2). GHRP-2 attenuated PDD-induced expression of protein and mRNA, the promoter activity of COX-2 and IL-8 genes, and the secretion of prostaglandin E2 (PGE2) and IL-8. GHRP-2 promoted the degradation of PDD-induced COX-2 and IL-8 proteins with the involvement of proteasomal and lysosomal pathways. PDD-mediated COX-2 production acts via the p38, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways; PDD-mediated IL-8 production acts via the p38, JNK and ERK pathways. GHRP-2 reduced the PDD-induced phosphorylation of p38 and JNK and activator protein 1 (AP-1) reporter activation and PDD-induced NF-κB nuclear translocation and reporter activation. The inhibitors of mitogen-activated protein kinase phosphatase-1 (MKP-1) and protein phosphatase 2 (PP2A) reduced the inhibitory effect of GHRP-2 on PDD-induced COX-2 and IL-8 expression. Our findings demonstrate an anti-inflammatory role for ghrelin (GHRP-2) in PKC-mediated inflammation of granulosa cells, at least in part, due to its inhibitory effect on PKC-induced activation of p38, JNK and NF-κB, possibly by targeting to MKP-1 and PP2A.

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Section: Discussionsupporting

confidence: 93%

Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells

Chao

Sun

Peng

et al. 2016

IJMS

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“…On those days, Graafian follicles can be observed in the ovary. Indeed, a previous study showed that ghrelin expression increases with follicle size, and peaks in the dominant or preovulatory follicle in buffalo (Gupta et al, 2015). Ghrelin was further found to be expressed in the granulosa and theca cells and to inhibit estradiol secretion, aromatase expression and apoptosis, while having a stimulatory effect on cell proliferation of buffalo granulosa cells (Gupta et al, 2015).…”

Section: Discussionmentioning

confidence: 94%

“…Indeed, a previous study showed that ghrelin expression increases with follicle size, and peaks in the dominant or preovulatory follicle in buffalo (Gupta et al, 2015). Ghrelin was further found to be expressed in the granulosa and theca cells and to inhibit estradiol secretion, aromatase expression and apoptosis, while having a stimulatory effect on cell proliferation of buffalo granulosa cells (Gupta et al, 2015). These data, combined with our results, suggest a role for ghrelin in reducing estradiol levels toward the LH surge and ovulation and inducing granulosa cell proliferation toward their conversion into luteal cells of the corpus luteum.…”

Section: Discussionmentioning

confidence: 98%

Seasonal and parity effects on ghrelin levels throughout the estrous cycle in dairy cows

Honig

Ofer

Elbaz

et al. 2016

General and Comparative Endocrinology

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“…The FF was stored at −20°C until the determination of P 4 and E 2 . As healthy follicles have relatively constant P 4 concentrations in FF, only follicles with P 4 below 100 ng/ml FF were used for the evaluation, to exclude atretic follicles (Gupta et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The role of pituitary gonadotropins and growth hormone is well established in the process of follicular and luteal dynamics. Nevertheless, recent evidences also proved the essential modulatory role by locally produced factors such as steroid hormones, peptides and growth factors like vascular endothelial growth factor (VEGF), IGF‐I, fibroblast growth factor (FGF), angiopoietin, thrombopoietin, ghrelin in the development of follicle, CL and regulation of reproductive cycle (Ali et al, ; Babitha et al, ; Berisha & Schams, ; Berisha, Schams, Kosmann, Amselgruber, & Einspanier, ; Chouhan et al, , ; Gupta et al, ; Gupta, Dangi, Singh, & Sarkar, ; Mishra et al, ; Sarkar, Schilffarth, Schams, Meyer, & Berisha, ; Uniyal et al, ). These locally produced compounds constitute a complex intraovarian autocrine, paracrine and juxtacrine regulatory system.…”

Section: Introductionmentioning

confidence: 99%

Expression and localization of adiponectin and its receptors in ovarian follicles during different stages of development and the modulatory effect of adiponectin on steroid production in water buffalo

Gupta¹,

Bahiram²,

Sardar³

et al. 2019

Reprod Domestic Animals

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Adiponectin is an adipocyte-derived hormone regulating energy metabolism, insulin sensitivity and recently found to regulate reproduction. The current study was carried out to investigate gene and protein expression, immunolocalization of adiponectin and its receptors AdipoR1 and AdipoR2 in ovarian follicles of different developmental stages in water buffalo (Bubalus bubalis) and to investigate the effect of adiponectin on steroid production in cultured bubaline granulosa cells. qPCR, western blotting and immunohistochemistry were applied to demonstrate mRNA expression, protein expression and immunolocalization, respectively. The results indicate that adiponectin, AdipoR1 and AdipoR2 were present in granulosa cells (GC) and theca interna (TI) of ovarian follicles and the expression of adiponectin, AdipoR1, AdipoR2 in GC and AdipoR1 and AdipoR2 in TI increased with increase in follicle size (p < .05). Expression of adiponectin was high in small and medium size follicles in TI. The adiponectin and its receptors were immunolocalized in the cytoplasm of GC and TI cells. Further, in the in-vitro study, GCs were cultured and treated with recombinant adiponectin each at 0, 1 and 10 µg/ml alone or with follicle stimulating hormone (FSH) at 30 ng/ml) or Insulin-like growth factor I (IGF-I) at 10 ng/ml for 48 hr after obtaining 75%-80%s confluency. Adiponectin at 10 µg/ml increased IGF-I-induced estradiol (E 2 ) and progesterone (P 4 ) secretion and FSH-induced E 2 secretion from GC and also increased the abundance of factors involved in E 2 and P 4 production (cytochrome P45019A1 [CYP19A1] and 3-beta-hydroxysteroid dehydrogenase [3β-HSD]). In conclusion, this study provides novel evidence for the presence of adiponectin and its receptors in ovarian follicles and modulatory role of adiponectin on steroid production in buffalo.

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Expression and localization of ghrelin and its receptor in ovarian follicles during different stages of development and the modulatory effect of ghrelin on granulosa cells function in buffalo

Cited by 15 publications

References 39 publications

Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells

Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells

Seasonal and parity effects on ghrelin levels throughout the estrous cycle in dairy cows

Expression and localization of adiponectin and its receptors in ovarian follicles during different stages of development and the modulatory effect of adiponectin on steroid production in water buffalo

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