Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis

Zou, Sheng Wei; Zhang, Jian Chao; Zhang, Xiao Dong; Miao, Shiying; Zong, Shu Dong; Sheng, Qi; Wang, Lin Fang

doi:10.1038/sj.cr.7290161

Cited by 39 publications

(27 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To our knowledge, the relevance of VCX/Y to cancer has not been previously investigated. VCX/Y proteins are localized in the nucleus and may be involved in regulating ribosome assembly during spermatogenesis (32), inhibiting mRNA decapping and regulating mRNA stability (10). In our study, VCX siRNA inhibited cell proliferation in PC-9 cells, suggesting that VCX/Y genes have oncogenic roles.…”

Section: Discussionmentioning

confidence: 99%

A Search for Novel Cancer/Testis Antigens in Lung Cancer Identifies VCX/Y Genes, Expanding the Repertoire of Potential Immunotherapeutic Targets

et al. 2014

View full text Add to dashboard Cite

Cancer/testis (CT) antigens are potential immunotherapeutic targets in cancer. However, the expression of particular antigens is limited to a subset of tumors of a given type. Thus, there is a need to identify antigens with complementary expression patterns for effective therapeutic intervention. In this study, we searched for genes that were distinctly expressed at a higher level in lung tumor tissue and the testes compared to other non-tumor tissues and identified members of the VCX/Y gene family as novel CT antigens. VCX3A, a member of the VCX/Y gene family, was expressed at the protein level in approximately 20% of lung adenocarcinomas and 35% of squamous cell carcinomas, but not expressed in normal lung tissues. Among CT antigens with concordant mRNA and protein expression levels, four CT antigens, XAGE1, VCX, IL13RA2, and SYCE1, were expressed, alone or in combination, in about 80% of lung adenocarcinoma tumors. The CT antigen VCX/Y gene family broadens the spectrum of CT antigens expressed in lung adenocarcinomas for clinical applications.

show abstract

Section: Discussionmentioning

confidence: 99%

A Search for Novel Cancer/Testis Antigens in Lung Cancer Identifies VCX/Y Genes, Expanding the Repertoire of Potential Immunotherapeutic Targets

et al. 2014

View full text Add to dashboard Cite

show abstract

“…VCX gene expression was reported to be restricted to the testis (Fukami et al, 2000; Lahn and Page, 2000; Zou et al, 2003). Nevertheless, we recently reported the cloning of the VCX-A gene product from human K562 erythroleukemia cells (Jiao et al, 2006), suggesting perhaps a preferential, rather than exclusive, expression in the testis.…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, by RT-PCR, the Y chromosome localized VCY genes are exclusively and robustly expressed in male germ cells (Figure 1A). Despite the reported testis germ cell restriction of the VCX genes (Fukami et al, 2000; Lahn and Page, 2000; Zou et al, 2003), we initially cloned the cDNA of VCX-A from human K562 erythroleukemia cells (Jiao et al, 2006). The presence of the VCX-A mRNA in erythroleukemia cells indicated that expression of this gene is not restricted to testis.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Neuritogenesis by a Protein Implicated in X-Linked Mental Retardation

Jiao¹,

Chen²,

Chen³

et al. 2009

J. Neurosci.

View full text Add to dashboard Cite

Posttranscriptional regulation is an important control mechanism governing gene expression in neurons. We recently demonstrated that VCX-A, a protein implicated in X-linked mental retardation, is an RNA-binding protein that specifically binds the 5Ј end of capped mRNAs to prevent their decapping and decay. Previously, expression of VCX-A was reported to be testes restricted. Consistent with a role in cognitive function, we demonstrate that VCX-A is ubiquitously expressed in human tissues including the brain. Moreover, retinoic acid-induced differentiation of human SH-SY5Y neuroblastoma cells promoted the accumulation of VCX-A in distinct cytoplasmic foci within neurites that colocalize with staufen1-containing RNA granules, suggesting a role in translational suppression and/or mRNA transport. Exogenous expression of VCX-A in rat primary hippocampal neurons, which normally do not express the primate-restricted VCX proteins, promoted neurite arborization, and shRNA-directed knockdown of the VCX genes in SH-SY5Y cells resulted in a reduction of both primary and secondary neurite projections upon differentiation. We propose that the cap-binding property of VCX-A reflects a role of this protein in mRNA translational regulation. In support of this hypothesized role, we demonstrate that VCX-A can specifically bind a subset of mRNAs involved in neuritogenesis and is also capable of promoting translational silencing. Thus, VCX-A contains the capacity to modulate the stability and translation of a subset of target mRNAs involved in neuronal differentiation and arborization. It is plausible that defects of these functions in the absence of the VCX genes could contribute to a mental retardation phenotype.

show abstract

“…For instance, DAZ-family members have been shown to bind to specific transcripts in vivo and regulate pre-meiotic translation of important factors such as the mouse VASA-homolog MVH (Reynolds et al 2005), which in turn plays a major role in the processing of PIWI-interacting RNAs (Kuramochi-Miyagawa et al 2010). RNA binding has also been observed for the splicing-associated RBMY (Elliott et al 2000), while other genes residing within the AZFb locus exhibit transcription factor activity (HSFY; Tessari et al 2004) or are involved in ribosome assembly (VCY; Zou et al 2003). Hence, impairment of the testicular transcriptional network by altering the function of transcription factors through modulation of their mRNAs or their translation could play a putative role.…”

Section: Discussionmentioning

confidence: 99%

A biopsy sample reduction approach to identify significant alterations of the testicular transcriptome in the presence of Y-chromosomal microdeletions that are independent of germ cell composition

et al. 2010

View full text Add to dashboard Cite

Y-chromosomal microdeletions (YCMD) are the major genetic cause of male infertility. To date, it is not known which global changes are induced by the presence of AZFc or AZFb + c deletions in the human testicular transcriptome. We investigated this question by microarray analysis in which we had to eliminate the ‘germ cell effect’, i.e., the dominating effect of germ cell transcripts due to the quantitative difference in germ cell composition in samples with/without YCMD. This problem was tackled by selecting 26 samples from an initial cohort of 34 samples by their homogeneity in respect to cellular composition as obtained from gene expression clustering. This way, the ‘germ cell effect’ was minimized, and a distinct ‘deletion effect’ became more apparent. Several hundred genes are influenced by YCMD as shown on the three different phenotypes hypospermatogenesis, meiotic arrest, and Sertoli-cell only syndrome. We validated on an independent cohort of samples five genes by quantitative real-time PCR that are expressed in germ cells or the somatic compartment and which are exclusively altered by the presence of YCMD. We conclude that the deletion of Y-chromosomal genes has a significant effect on spermatogenesis by modulating the transcriptional network of the germ cell and somatic compartment.Electronic supplementary materialThe online version of this article (doi:10.1007/s00439-010-0865-9) contains supplementary material, which is available to authorized users.

show abstract

Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis

Cited by 39 publications

References 13 publications

A Search for Novel Cancer/Testis Antigens in Lung Cancer Identifies VCX/Y Genes, Expanding the Repertoire of Potential Immunotherapeutic Targets

A Search for Novel Cancer/Testis Antigens in Lung Cancer Identifies VCX/Y Genes, Expanding the Repertoire of Potential Immunotherapeutic Targets

Modulation of Neuritogenesis by a Protein Implicated in X-Linked Mental Retardation

A biopsy sample reduction approach to identify significant alterations of the testicular transcriptome in the presence of Y-chromosomal microdeletions that are independent of germ cell composition

Contact Info

Product

Resources

About