Expression Hosts for Enzyme Discovery and Production

Streamlined Preparation of Immobilized Candida antarctica Lipase B

“…38 By contrast, prokaryotic host systems, such as E. coli, are known to provide nonglycosylated proteins. 39 CalB is a monomeric enzyme containing three disulphide bonds and one N-glycosylation sequon, 7,40 and it is therefore not an ideal candidate for E. coli expression. However, mutagenesis has been used to explore the lack of glycosylation on CalB.…”