Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue

Bründl, Johannes; Wallinger, Sabine; Breyer, Johannes; Weber, Florian; Evert, Matthias; Georgopoulos, Nikolaos T.; Rosenhammer, Bernd; Burger, Maximilian; Otto, Wolfgang; Rubenwolf, Peter

doi:10.1186/s12894-018-0391-y

Cited by 19 publications

(21 citation statements)

References 28 publications

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“…It demonstrated that the AQP5 transcripts were not limited to the site of implantation. In previous studies, we suggested the possibility of luminal cells transdifferentiation into cells of mesenchymal characteristics, which could explain the presence of AQP5 on the first day of culture [30,31].…”

Section: Discussionmentioning

confidence: 88%

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

Wojtanowicz-Markiewicz

Kulus

Knap

et al. 2020

BioMed Research International

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Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx’s behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.

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Section: Discussionmentioning

confidence: 88%

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

Wojtanowicz-Markiewicz

Kulus

Knap

et al. 2020

BioMed Research International

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“…Development of gastric adenocarcinomas is a stepwise progression of histologic lesions, starting with gastritis, followed by intestinal metaplasia, dysplasia, and eventually invasive adenocarcinoma. AQP3 has been reported to associate with human cancer progression and metastasis, such as pancreatic ductal adenocarcinoma, prostate cancer, cervical intraepithelial neoplasia, and triple-negative breast cancer [22][23][24][25][26]. However, the role of AQP3 and its expression pattern in gastric carcinogenesis remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Aquaporin 3 Expression Pattern in Gastric Diseases and its significance

Dai²,

Dou

et al. 2020

Preprint

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Background Aquaporin 3(AQP3) has been implicated in gastric intestinal metaplasia and gastric cancer, and considered as a biomarker to improve treatment strategy. Accumulating evidence suggests that AQP3 is involved in the gastric carcinogenesis and the disease progression. However, whether AQP3 is involved in the transformation from gastritis to gastric cancer remain elusive. In this study, we intended to realized the expression pattern and its significance of AQP3 in different gastric diseases. Methods A total of 101 patients diagnosed with gastric diseases were included in the study. A gastric tissue biopsy was taken from the gastric antrum during endoscopic examination. Expression of AQP3 protein is determined by immunohistochemistry using polyclonal rabbit anti-AQP3 antibody. Percentage of positive cells and staining intensity were counted and measured. Results The frequency of AQP3 positivity was similar between the disease types of chronic gastritis, gastric ulcer, gastric erosion, and atrophic gastritis, whereas the frequency of AQP3 positivity was significantly higher in patients with gastric intestinal metaplasia、gastric dysplasia 、gastric polyps and intestinal-type gastric adenocarcinoma than that in patients with gastritis, gastric ulcer, gastric erosion, or atrophic gastritis(p<0.0001, p=0.001, p=0.006, p=0.0009, respectively), especially in the patients with hyperplastic polyps (p<0.0001). Conclusion The frequency of AQP3 positivity was significantly higher in patients with gastric intestinal metaplasia, gastric dysplasia, and gastric adenocarcinoma, and the frequency between them was similar, suggesting that AQP3 expression is peaked at the stage of gastric intestinal metaplasia, which further confirmed that gastric intestinal metaplasia is a pivotal progression in gastric carcinogenesis pathologically. Interestingly, it is the first time to report AQP3 positive expression in patients with gastric polyps. In short, AQP3 is involved in the progression from gastritis to gastric adenocarcinoma, and might be a potential biomarker to improve the treatment strategy of gastric cancer.

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“…Our results were similar to the studies using anti-AQP3 antibodies prepared from AA180-228 and C terminus peptide of AQP3 in the SCCs of the lung, skin, and oral cavity, UC, prostate adenocarcinoma, and bronchioloalveolar carcinoma (Table 5 ). 17 , 18 , 20 , 29 – 32 , 34 , 35 , 38 , 39 Degradation of AQP3 protein located near the C terminus part in UC and AA180-228 part of AQP3 in OSCC is associated with a higher grade of the tumors and tumor cell invasion (muscle-invasive UC and OSCC with lymphatic metastasis cases), the underlying molecular mechanism of which remains unclear. 17 , 20 , 34 However, our results were contradictory to those of studies on medullary thyroid carcinoma, hepatocellular carcinoma, pancreatic ductal adenocarcinoma, and triple-negative breast cancer, which suggested increased expression of AQP3 in these carcinomas (Table 5 ).…”

Section: Discussionmentioning

confidence: 99%

Assessing the Expression of Aquaporin 3 Antigen-Recognition Sites in Oral Squamous Cell Carcinoma

Udompatanakorn

Yada

Matsuo

2019

Applied Immunohistochemistry &Amp; Molecular Morphology

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Aquaporin 3 (AQP3) serves as a water and glycerol transporter facilitating epithelial cell hydration. Recently, the involvement of AQP3 in cancers has been reported. However, the immunohistochemical expression of AQP3 in carcinomas remains controversial. We hypothesized that differences in aquaporin 3 antigen recognition (AQP3 AR) may influence their expressions. Thus, our study aimed to assess the immunostaining patterns of 3 AQP3 AR sites in oral squamous cell carcinoma (OSCC) and to compare the adjacent areas of high-grade epithelial dysplasia (HG-ED) and normal oral mucosa (NOM). The study group included formalin-fixed OSCC samples (n=51) with adjacent regions of HG-ED (n=12) and NOM (n=51). The tissues were stained with anti-AQP3 antibodies (AR sites at amino acid (AA) 250-C terminus, AA180-228, and N terminus AA1-80) by immunohistochemistry. Our results showed that strong membranous immunostaining was observed for AQP3 AR sites at the AA250-C terminus and AA180-228 in all the samples for NOM and weak AQP3 immunostaining for both the AR sites in all the 12 samples for HG-ED. The invasive front of OSCC samples showed that AQP3 AR at the AA250-C terminus decreased in 42/51 samples (82.4%) and AA180-228 in 47/51 samples (92.2%). Conversely, in the AQP3 AR site at N terminus AA1-80, all samples of the NOM showed negative or slightly positive staining in the cytoplasm of the lower layers. AQP3 expression was increased in 12/12 cases (100%) and 46/51 cases (90.2%) in the HG-ED and invasive front of OSCC, respectively. AQP3 may be used as a biomarker for detecting malignant transformations. AQP3 AR site differences influence their immunohistochemical expression in OSCC.

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Expression, localisation and potential significance of aquaporins in benign and malignant human prostate tissue

Cited by 19 publications

References 28 publications

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

Aquaporin 3 Expression Pattern in Gastric Diseases and its significance

Assessing the Expression of Aquaporin 3 Antigen-Recognition Sites in Oral Squamous Cell Carcinoma

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