2016
DOI: 10.1016/j.neulet.2016.06.030
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Expression of a dynamin 2 mutant associated with Charcot-Marie-Tooth disease leads to aberrant actin dynamics and lamellipodia formation

Abstract: Specific mutations in dynamin 2 are linked to Charcot-Marie-Tooth disease (CMT), an inherited peripheral neuropathy. However, the effects of these mutations on dynamin function, particularly in relation to the regulation of the actin cytoskeleton remain unclear. Here, selected CMT-associated dynamin mutants were expressed to examine their role in the pathogenesis of CMT in U2OS cells. Ectopic expression of the dynamin CMT mutants 555Δ3 and K562E caused an approximately 50% decrease in serum stimulation-depende… Show more

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Cited by 7 publications
(8 citation statements)
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“…43). Recent results indicate a critical role for dynamin and actin (63)(64)(65), facilitated by cortactin (44 -47), in coordinating cytoskeletal remodeling, thus linking our findings of inhibition of dynamin-dependent endocytosis of MVs with CSE-mediated oxidation of actin polymerization (Fig. 5, E and F).…”
Section: Mechanisms and Modulation Of Alveolar Microvesicle Uptakesupporting
confidence: 85%
“…43). Recent results indicate a critical role for dynamin and actin (63)(64)(65), facilitated by cortactin (44 -47), in coordinating cytoskeletal remodeling, thus linking our findings of inhibition of dynamin-dependent endocytosis of MVs with CSE-mediated oxidation of actin polymerization (Fig. 5, E and F).…”
Section: Mechanisms and Modulation Of Alveolar Microvesicle Uptakesupporting
confidence: 85%
“…Alterations in stability and dynamic arrangements of actin filaments or microtubules affect SC process extension and radial sorting (Grove et al, 2007; Sparrow et al, 2012) and/or myelination (Jin et al, 2011; Novak et al, 2011). Since DNM2 is involved in regulating actin and microtubule dynamics and stability (Gu et al, 2010; Tanabe and Takei, 2009; Yamada et al, 2016), potential cytoskeleton abnormalities in P0-Dnm2 KO SCs may also partially contribute to the impaired radial sorting phenotype.…”
Section: Discussionmentioning
confidence: 99%
“…Our previous studies show that dynamin 2-K562E, a point mutation in dynamin 2 PH domain, induces aberrant actin filament structures in U2OS cells ( Yamada et al, 2016b ). To investigate the mode of action for dynamin 2 mutation-triggered changes in actin morphology in HPCs, we first examined actin filament distribution in dynamin 2-WT- or K562E-expressing cells by double-immunofluorescence microscopy.…”
Section: Resultsmentioning
confidence: 99%
“…Rat dynamin 2-WT, 555Δ3, and K562E cloned into pcDNA4 V5/His are described in Yamada et al (2016b) . Rat dynamin 2-WT was subcloned into pIRES2-DsRed2 as an EcoRI-SmaI fragment (Clontech Laboratories).…”
Section: Experimental Methodsmentioning
confidence: 99%
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