Expression of Adhesion Molecules on CD34 ⁺ Cells in Peripheral Blood of Non‐Hodgkin's Lymphoma Patients Mobilized with Different Growth Factors

Abstract

The mean percentage of CD34 + cells expressing VLA-4 in the BM and PB was relatively high (73.4% ± 12% and 65.4% ± 6.6%, respectively) and dropped considerably in the PBSC collections to 43.5% ± 7.1% with a similar pattern observed for patients in arms A, B, and C. However, when the patients were regrouped as "good mobilizers" and "poor mobilizers," a higher percentage of CD34 + cells expressing VLA-4 was observed in the PBSC of the pooled "good mobilizers" (50.5% ± 9% versus 36.3% ± 6.4%; p = 0.01). We conclude that release of CD34 + cells to the PB involves a general downregulation of Thy1, L-selectin and VLA-4 on CD34 + cells, irrespective of the growth factor used for mobilization. However, good mobilizers had a relatively higher percentage of CD34 + cells expressing the VLA-4 antigen.

Decreased Stroma Adhesion Capacity of CD34+Progenitor Cells from Mobilized Peripheral Blood Is Not Lineage- or Stage-Specific and Is Associated with Lowβ1 andβ2 Integrin Expression

“…In our study, we found decreased levels of a4 and b1 integrin chains expressed on mobilized PB CD34 1 cells compared to BM cells, as previously reported (11,21,(23)(24)(25), together with decreased stroma adhesion capacity to BM stroma. This emphasizes the crucial role of VLA-4 in maintaining HPC within the BM microenvironment, as directly demonstrated by others (3)(4)(5).…”

Recent Developments in the Regulation of Peripheral Blood Stem Cell Mobilization and Engraftment by Cytokines, Chemokines, and Adhesion Molecules

“…In recent studies, we also observed a decrease in the co-expression of VLA-4 and L-selectin on CD34 1 cells in the apheresis collections of NHL patients mobilized with cyclophosphamide plus G-CSF, GM-CSF, and GM-CSF followed by G-CSF, compared to CD34 1 cells in the steady-state bone marrow and peripheral blood (58,59). Furthermore, the decreases in the expression of VLA-4 and L-selectin on CD34 1 cells was observed in the apheresis collections of all patients, regardless of the growth factor used for mobilization.…”

Improved Mobilization of Peripheral Blood CD34+Cells and Dendritic Cells by AMD3100 plus Granulocyte–Colony-Stimulating Factor in Non-Hodgkin's Lymphoma Patients

“…I T IS WIDELY ESTABLISHED that mobilization of peripheral blood stem cells (PBSCs) with hematopoietic growth factors such as granulocyte-colony-stimulating factor (G-CSF) or granulocyte macrophage (GM-CSF) involves down regulation of adhesion molecules such as VLA4, VLA5, CD44, and L-selectin (1)(2)(3)(4)(5)(6). Stromal derived factor-1 (SDF-1) is a chemokine and plays an important role in chemotaxis of many blood cells and in survival and proliferation of hematopoietic stem cells through activation of its specific receptor CXCR4.…”