Expression of adhesion molecules on mature cholangiocytes in canal of Hering and bile ductules in wedge biopsy samples of primary biliary cirrhosis

Abstract

Abstract Abstract Abstract AIM:To examine the expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) expression on canals of Hering (CoH) and bile ductules associated with the autoimmune process of bile duct destruction in primary biliary cirrhosis (PBC). METHODS:Ten wedged liver biopsies of PBC (five cases each of stages 2 and 3) were studied. The liver specimens were processed for transmission electron microscopy. Immunohistochemistry was performed using anti-ICAM-1 and anti-LFA-1 mouse mAbs. In situ hybridization was done to examine the messenger RNA expression of ICAM-1 in formalin-fixed, paraffin-embedded sections using peptide nucleic acid probes and the catalyzed signal amplification (CSA) technique. Immunogold-silver staining for electron microscopy was performed using anti-ICAM and anti-LFA-1 mouse mAbs. The immunogold particles on epithelial cells of bile ductules and cholangiocytes of CoH cells were counted and analyzed semi-quantitatively. Western blotting was performed to confirm ICAM-1 protein expression. RESULTS: CONCLUSION:De novo expression of ICAM-1 both on mature cholangiocytes in CoH and epithelial cells in bile ductules in PBC implies that lymphocyte-induced destruction through adhesion by ICAM-1 and binding of LFA-1-expressing activated lymphocytes takes place not only in bile ductules but also in the CoH.

Vascular cell adhesion molecule 1 as a predictor of severe osteoarthritis of the hip and knee joints

“…Taken together, these previous findings suggest that CXCL12 might be involved in the formation of the coronal arrangement of CD38 + cells around bile ducts. Other potential contributing factors for the formation of coronal arrangement include hepatocyte growth factor, 49 lymphocyte function–associated antigen‐1 (LFA‐1)/ intercellular adhesion molecule‐1 (ICAM‐1), 50, 51 CXCL13, and CXCL5, 52 which should also be explored in future studies.…”

Effect of thalidomide dithiocarbamate analogs on the intercellular adhesion molecule-1 expression

“…Smith and Thomas [45] have previously reported the expression of ICAM-1 in liver hepatocytes and kidney tubules. Up-regulated ICAM-1 expression in chronic liver disease has been reported in several studies [46,47]. ICAM-1 expression in metastatic liver cancer has a positive correlation with tumor size; it increases as metastasis occurs, and then maintained high [48].…”

The Pathogenesis of Primary Biliary Cholangitis: A Comprehensive Review

“…41 IgA transcytosis has been demonstrated to be involved in PBC pathogenesis. 42 Importantly, BECs express toll-like receptors (TLRs) and antimicrobial peptides 43 ; act as antigen-presenting cells (APCs) by expressing human leukocyte antigen (HLA) molecules and costimulatory molecules [44][45][46] ; recruit leukocytes to the target site by expressing adhesion molecules, 47,48 cytokines, and chemokines 49,50 ; and induce apoptosis of leukocytes to limit the immune responses. 51 In response to injury, BECs proliferate, change their phenotype, and become active players in the immune response 52 (►Table 1).…”