Expression of adiponectin and adiponectin receptors 1 and 2 in the porcine uterus, conceptus, and trophoblast during early pregnancy

Smolińska, Nina; Maleszka, Anna; Kieżun, Marta; Szeszko, Karol; Kamiński, Tadeusz

doi:10.1016/j.theriogenology.2014.07.018

Cited by 28 publications

(28 citation statements)

References 39 publications

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“…Expression of adiponectin and its receptors has been reported for porcine endometria during the estrous cycle and early pregnancy (Smolinska et al, ,). To determine temporal and cell‐specific expression of ADIPOR1 and ADIPOR2 for cyclic and pregnant pigs, we performed quantitative RT‐PCR and in situ hybridization analyses.…”

Section: Resultsmentioning

confidence: 99%

“…In mice, there is adiponectin signaling associated with embryonic development and uterine receptivity as there is abundant expression of adiponectin and its receptors in trophoblast stem cells and decidual cells during the preimplantation period of pregnancy (Kim et al, ). Furthermore, adiponectin and its receptors are expressed in endometria and conceptuses during early pregnancy in pigs in support of implantation (Smolinska et al, ). However, inhibitory effects of parity on expression of adiponectin in the uterus are associated with a reduction in litter size and uterine receptivity to implantation have not been identified.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, in human placentae and maternal adipose tissues, adiponectin stimulates production of pro‐inflammatory cytokines such as interleukin‐1 beta (IL‐1β), IL‐6, and tumor necrosis factor alpha (TNF‐α) through nuclear factor‐kappa B (NF‐κB), PPARγ, and ERK1/2 MAPK (Lappas, Permezel, & Rice, ). However, adiponectin‐mediated signal transduction in porcine endometrium has not been identified previously even though there is abundant expression of adiponectin and its receptors in uterine LE and stroma cells during the peri‐implantation period of pregnancy (Smolinska et al, ). Therefore, in the present study, we demonstrated that adiponectin activated phosphorylation of PI3 K (AKT, P70S6K, and S6) and MAPK (ERK1/2, P38, and JNK) proteins in pLE cells in a time‐dependent manner and that inhibition of PI3K, ERK1/2, P38, and JNK signaling reduced proliferation of pLE cells.…”

Section: Discussionmentioning

confidence: 99%

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A critical role for adiponectin‐mediated development of endometrial luminal epithelial cells during the peri‐implantation period of pregnancy

Lim

Choi

Bae

et al. 2017

Journal Cellular Physiology

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Adiponectin is one of the adipokines in the collagen superfamily. It is secreted primarily by white adipocytes and influences reproductive processes including ovarian and uterine functions. Adiponectin regulates energy homeostasis, insulin sensitivity, and is anti-inflammatory in various tissues. Its receptors (ADIPOR1 and ADIPOR2) are widely expressed in mammalian tissues, including porcine conceptuses and endometrial during the estrous cycle and peri-implantation period of pregnancy. However, regulatory effects of adiponectin on endometrial epithelial cells are unknown. Therefore, we investigated the effects of parity on expression of ADIPOR1 and ADIPOR2 and the effects of adiponectin in the porcine endometrium during early pregnancy. Results of this study revealed robust expression of ADIPOR1 and ADIPOR2 in uterine luminal (LE) and glandular (GE) epithelia during early pregnancy and expression decreased as with increasing parity. For porcine luminal epithelial (pLE) cells, adiponectin enhanced proliferation, and increased phosphorylation of AKT, P70S6K, S6, ERK1/2, JNK, P38, and P90RSK in a time-dependent manner. Moreover, the abundance of adiponectin-activated signaling molecules were suppressed by pharmacological inhibitors including wortmannin, U0126, SP600125, and SB203580, respectively, in pLE cells. Furthermore, inhibition of each targeted signal transduction molecule influenced proliferation of adiponectin-stimulated pLE cells. In addition, adiponectin inhibited tunicamycin-induced endoplasmic reticulum (ER)-stress through effects on ER stress regulated proteins in pLE cells. Collectively, these results suggest that adiponectin affects development of porcine uterine epithelia and reproductive performance through modulation of PI3K/AKT and MAPK cell signaling pathways.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A critical role for adiponectin‐mediated development of endometrial luminal epithelial cells during the peri‐implantation period of pregnancy

Lim

Choi

Bae

et al. 2017

Journal Cellular Physiology

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“…Expression of the adiponectin system in the uterus has been observed in mouse, pig, human, and other species during pregnancy, embryo implantation, and the estrous cycle. [242526] Previous studies showed differences in the protein expression of the adiponectin system during the estrous cycle and in early gestation, which demonstrated that the expression of adiponectin system could be modulated by steroid hormones, such as estrone and estradiol. [2627] The adiponectin mRNA levels in HFD- and E 2 -treated rats were higher than HFD rats, and a similar trend was observed in E 2 -treated rats in comparison with control group.…”

Section: Discussionmentioning

confidence: 99%

Effect of High-fat Diet-induced Disorders on Rat with Endometrial Hyperplasia and Adiponectin System in Circulation and Uterus

Shang

Liu

Wang

et al. 2017

Chinese Medical Journal

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Background:Epidemiologic and genetic studies suggest a link between insulin resistance (IR) and endometrial cancer, and endometrial hyperplasia (EH) is a precancerous stage of endometrial cancer. Adiponectin is an adipokine which previously shown to be a risk factor for endometrial cancer. The aim of the study was to develop a rat model of IR and EH and evaluate adiponectin system in circulation and uterus.Methods:This study was a 46-week animal trial from February 2014 to January 2015. Female Sprague-Dawley rats were fed with high-fat diet (HFD) for 40 weeks to induce IR. Followed by ovariectomization, rats were orally administrated to 17β-estradiol (E2) for 4 weeks to induce EH and then sacrificed. A total of 36 rats were divided into four groups: E2, HFD, HFD + E2, and control groups. Data were analyzed with Student's t-test, one-way analysis of variance (ANOVA), and Mann-Whitney U-tests. Chi-square was used to evaluate the score of immunohistochemistry.Results:The thickness of endometrial, glandular epithelium, and myometrium in the HFD-E2 group were higher than the E2 group (F = 59.02, F = 23.51 and F = 12.53, respectively, all P < 0.001). Plasma adiponectin levels in the E2 group were lower than those in the control group, and the levels in the HFD-E2 group were lower than those in the HFD group (F = 13.15, P < 0.05). However, after normalized to visceral adipose tissue, compared to the control group, plasma adiponectin levels were decreased in rat with HFD in the absence or presence of E2, respectively (F = 6.72, P < 0.05). Adiponectin gene (F = 10.48, P < 0.05) and protein (P < 0.05) levels in uterus in the HFD-E2 group were higher than those in the HFD group.Conclusions:This study manifests that IR can effectively modulate EH, which suggests the involvement of energetic metabolism in uterine alternation. The combination effects of IR and EH modulate circulating adiponectin levels. However, adiponectin gene and protein levels in uterus are mainly response to estradiol.

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“…Female reproductive functions are closely associated with nutritional status, and adiponectin seems to be an important factor linking the energy homoeostasis with reproductive function. The impact of adiponectin in the endometrium in productive species is almost unknown (Smolinska et al., ), and as far as we know this is the first description of adiponectin receptors in bovine uterus. In humans (Takemura et al., ) and rodent uterus (Mirkin et al., ), expression of adiponectin receptors ( ADIPOR1/2 ) increased during the window of implantation suggesting a relevant role during this period.…”

Section: Discussionmentioning

confidence: 94%

Endometrial gene expression in primiparous dairy cows at the end of the voluntary waiting period is affected by nutrition: Total mixed ration vs increasing levels of herbage allowance

Astessiano

Carriquiry

Mattiauda

et al. 2017

Reprod Domestic Animals

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The study postulated that differential nutritional management during the early lactation period would be reflected in endometrial expression of genes related to embryo growth at the end of the voluntary waiting period. Thus, the effect of the combined use of total mixed ration (TMR) and grazing under different herbage allowances during the first 75 days post-partum (DPP) on endometrial gene expression was evaluated in primiparous dairy cows. Cows were blocked by body weight, age and body condition score and randomly assigned to three grazing treatments: high (HA, 30 kg DM per cow per day), medium (MA, 15 kg DM per cow per day) and low (LA, 7.5 kg DM per cow per day) herbage allowance (mixed pasture, 2,600 kg DM per ha) plus 8 kg DM of supplement or TMR (55% forage, 45% concentrate) fed ad libitum (TMR) from calving to 75 DPP. At 57 DPP, cows were synchronized for oestrus (day 0, 68 DPP) and at day 7, endometrial biopsies were obtained. The nutritional treatment did not affect insulin, IGF-1 and leptin concentrations on days 0, 4 or 7. Expression of IGF1, IGFBP3, IGFBP4, ADIPOR1 and ADIPOR2 mRNA was significantly affected by the nutritional treatment. Endometrial IGF1 and IGFBP4 mRNA were twofold greater in TMR and HA than MA and LA cows. Expression of IGFBP3 and ADIPOR1 mRNAs was greater in TMR and HA than MA cows, but did not differ from LA cows. All groups had greater expression of ADIPOR2 mRNA than MA cows. This study provided solid evidence of the importance of nutritional management during early lactation on uterine environment at the end of the voluntary waiting period. The greater expression of genes related to embryo growth and uterine function (IGF system, progesterone and adiponectin receptors) in cows fed diets maximizing energy intake suggests a favourable environment for embryonic growth, which may explain the improved reproductive performance of cows in good energy balance.

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Expression of adiponectin and adiponectin receptors 1 and 2 in the porcine uterus, conceptus, and trophoblast during early pregnancy

Cited by 28 publications

References 39 publications

A critical role for adiponectin‐mediated development of endometrial luminal epithelial cells during the peri‐implantation period of pregnancy

A critical role for adiponectin‐mediated development of endometrial luminal epithelial cells during the peri‐implantation period of pregnancy

Effect of High-fat Diet-induced Disorders on Rat with Endometrial Hyperplasia and Adiponectin System in Circulation and Uterus

Endometrial gene expression in primiparous dairy cows at the end of the voluntary waiting period is affected by nutrition: Total mixed ration vs increasing levels of herbage allowance

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