Expression of an ABA responsive 21 kDa protein in finger millet (Eleusine coracana Gaertn.) under stress and its relevance in stress tolerance

Aarati, P; Krishnaprasad, B. T.; Ganeshkumar,; Savitha, M.S.; Ramaswamy, Gopalakrishna; Ramamohan, G.; Udayakumar, M.

doi:10.1016/s0168-9452(02)00321-7

Cited by 16 publications

(10 citation statements)

References 37 publications

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“…These data support previous results for rice, where no correlation was found between the levels of a SALT protein expression and the sensitivity to the stress (Filho et al, 2003). However, our results do not agree with findings in which salt-resistant species show higher levels of expression of a 21 kDa protein than the sensitive ones (Aarati et al, 2003).…”

Section: Biochemical Reaction Of Salt-resistant and Salt-sensitive Whcontrasting

confidence: 63%

“…Our previous results with maize show that the differentially expressed or post-translationally modified proteins are enzymes of carbon and nitrogen metabolism, but not specific proteins contributing to salt resistance . The biochemical reaction in the first phase of salt stress appears to be unspecific as many of the proteins identified in response to short-term salt stress are also induced in response to other stresses such as desiccation, low temperature, dehydration, and wounding (Aarati et al, 2003;Filho et al, 2003). Differential expression of proteins after stress treatment thus reflects the capability of metabolism to adjust to varying requirements, but is not necessarily related to resistance.…”

Section: Biochemical Reaction Of Salt-resistant and Salt-sensitive Whmentioning

confidence: 99%

“…A number of stress-induced proteins has been identified to-date. For example, a 21 kDa protein was induced under desiccation, low temperature, abscisic acid, and 400-600 mM NaCl for 36 h, and salt-resistant species showed higher levels of expression than sensitive ones (Aarati et al, 2003). A 23 kDa protein was significantly decreased by salt stress after 7, 14, and 30 d, and almost disappeared after 45 d of treatment in 400 mM NaCl (Parida et al, 2005).…”

Section: Introductionmentioning

confidence: 98%

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Salt‐resistant and salt‐sensitive wheat genotypes show similar biochemical reaction at protein level in the first phase of salt stress

Saqib

Zörb

Schubert

2006

Z. Pflanzenernähr. Bodenk.

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Salinity has a two-phase effect on plant growth, an osmotic effect due to salts in the outside solution and ion toxicity in a second phase due to salt build-up in transpiring leaves. To elucidate salt-resistance mechanisms in the first phase of salt stress, we studied the biochemical reaction of salt-resistant and salt-sensitive wheat (Triticum aestivum L.) genotypes at protein level after 10 d exposure to 125 mM-NaCl salinity (first phase of salt stress) and the variation of salt resistance among the genotypes after 30 d exposure to 125 mM-NaCl salinity (second phase of salt stress) in solution culture experiments in a growth chamber. The three genotypes differed significantly in absolute and relative shoot and root dry weights after 30 d exposure to NaCl salinity. SARC-1 produced the maximum and 7-Cerros the minimum shoot dry weights under salinity relative to control. A highly significant negative correlation (r 2 = -0.99) was observed between salt resistance (% shoot dry weight under salinity relative to control) and shoot Na + concentration of the wheat genotypes studied. However, the salt-resistant and salt-sensitive genotypes showed a similar biochemical reaction at the level of proteins after 10 d exposure to 125 mM NaCl. In both genotypes, the expression of more than 50% proteins was changed, but the difference between the genotypes in various categories of protein change (up-regulated, down-regulated, disappeared, and new-appeared) was only 1%-8%. It is concluded that the initial biochemical reaction to salinity at protein level in wheat is an unspecific response and not a specific adaptation to salinity.

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Section: Biochemical Reaction Of Salt-resistant and Salt-sensitive Whcontrasting

confidence: 63%

Section: Biochemical Reaction Of Salt-resistant and Salt-sensitive Whmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Salt‐resistant and salt‐sensitive wheat genotypes show similar biochemical reaction at protein level in the first phase of salt stress

Saqib

Zörb

Schubert

2006

Z. Pflanzenernähr. Bodenk.

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“…The expression of LEA 1 was observed in both control and 10% PEG induced stress seedlings at 12 and 24 h. Moreover, expression of this LEA 1 decreased in embryonic axis when compared to cotyledons at 24 h and also completely absent in embryonic axis at 48 h and in root and shoot at 72 h suggesting the differential regulation of LEA 1 under water stress in germinating seedlings of horsegram. Similar results were reported previously by Arati et al (2003) in ragi cultivars under osmotic stress.…”

Section: Discussionsupporting

confidence: 92%

Group 1 and 2 LEA protein expression correlates with a decrease in water stress induced protein aggregation in horsegram during germination and seedling growth

Veeranagamallaiah

Prasanthi

Reddy

et al. 2011

Journal of Plant Physiology

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“…PEG, NaCl and menadione induces osmotic, salinity and oxidative stresses respectively and solutions were prepared as described in previous literatures [27], [57], [58]. Menadione undergoes rapid auto-oxidation with the regeneration of the parent quinone and thus produces superoxide radicals and hydrogen peroxide [59], [60].…”

Section: Methodsmentioning

confidence: 99%

Stable Expression of mtlD Gene Imparts Multiple Stress Tolerance in Finger Millet

et al. 2014

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Finger millet is susceptible to abiotic stresses, especially drought and salinity stress, in the field during seed germination and early stages of seedling development. Therefore developing stress tolerant finger millet plants combating drought, salinity and associated oxidative stress in these two growth stages is important. Cellular protection through osmotic adjustment and efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms in plants. Mannitol, an osmolyte, is known to scavenge hydroxyl radicals generated during various abiotic stresses and thereby minimize stress damage in several plant species. In this study transgenic finger millet plants expressing the mannitol biosynthetic pathway gene from bacteria, mannitol-1-phosphate dehydrogenase (mtlD), were developed through Agrobacterium tumefaciens-mediated genetic transformation. mtlD gene integration in the putative transgenic plants was confirmed by Southern blot. Further, performance of transgenic finger millet under drought, salinity and oxidative stress was studied at plant level in T1 generation and in T1 and T2 generation seedlings. Results from these experiments showed that transgenic finger millet had better growth under drought and salinity stress compared to wild-type. At plant level, transgenic plants showed better osmotic adjustment and chlorophyll retention under drought stress compared to the wild-type. However, the overall increase in stress tolerance of transgenics for the three stresses, especially for oxidative stress, was only marginal compared to other mtlD gene expressing plant species reported in the literature. Moreover, the Agrobacterium-mediated genetic transformation protocol developed for finger millet in this study can be used to introduce diverse traits of agronomic importance in finger millet.

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Expression of an ABA responsive 21 kDa protein in finger millet (Eleusine coracana Gaertn.) under stress and its relevance in stress tolerance

Cited by 16 publications

References 37 publications

Salt‐resistant and salt‐sensitive wheat genotypes show similar biochemical reaction at protein level in the first phase of salt stress

Salt‐resistant and salt‐sensitive wheat genotypes show similar biochemical reaction at protein level in the first phase of salt stress

Group 1 and 2 LEA protein expression correlates with a decrease in water stress induced protein aggregation in horsegram during germination and seedling growth

Stable Expression of mtlD Gene Imparts Multiple Stress Tolerance in Finger Millet

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