Expression of bone morphogenetic protein‐10 mRNA during chicken heart development

Abstract

In this communication we describe the expression pattern of BMP10 mRNA during cardiac development in chickens. BMP10 is considered an important factor in the regulation of cardiac growth and trabeculation in the murine embryo. We identified chicken Ests, which are similar to mouse and human BMP10 in the UMIST database. The cDNA clone that contained most sequences was obtained, verified by sequence analysis, and used to determine the spatiotemporal pattern of gene expression. BMP10 mRNA is initially expressed at HH10 in the myocardium of the arterial pole of the heart tube, anterior to the interventricular groove. Between HH14 and HH22, BMP10 mRNA becomes broadly expressed in the outflow tract, the distal part of the inflow tract, and the trabeculated part of the developing ventricles and atria. From HH31 onward, BMP10 mRNA expression decreases in the ventricular myocardium by first disappearing from the compact myocardium and then from the tips of the trabecules. At HH44, BMP10 mRNA is expressed only in the trabeculated myocardium of the atria and the endocardium of the ventricles. The observed expression pattern of BMP10 mRNA suggests that it may play a role in regulating the formation of the ventricular wall and trabecules.

BMP Signals Promote Proepicardial Protrusion Necessary for Recruitment of Coronary Vessel and Epicardial Progenitors to the Heart

“…To test this possibility we examined BMP ligand expression in the heart. Of the five BMP family members expressed in the avian heart at stages of active PE protrusion (Somi et al, 2004a; Somi et al, 2004b; Teichmann and Kessel, 2004), we detected Bmp2 , Bmp5 and Bmp7 in the AV/IC region (Fig 3A–C, 3E, and 3F) with Bmp2 being highly enriched in this region and absent from the sinoatrium. Bmp2 is expressed in the heart as the PE forms (Figure 3A) and is localized to the AV region prior to attachment (Fig 3B and 3C).…”

Regulation of the ALK1 ligands, BMP9 and BMP10

“…In mouse embryos, high levels of BMP10 expression can be detected from around E8.5 [11], mostly restricted to the trabeculated myocardium of atria and ventricles, playing an important role in the trabeculation of the embryonic heart [15,16]. In adults, BMP10 is still highly expressed in the heart, but its expression is restricted to only the right atrium [10].…”

Role of bone morphogenetic proteins in cardiac differentiation

“…Ventricle formation becomes evident with the formation of trabecules at E8 in mice and stage 12 in chicken. Both in mice [98] and chicken [99] BMP10 expression becomes first evident in the heart tube at the site of chamber formation. Ventricle formation is absent in BMP10 knockout mice that die at E9 [98].…”