Expression of CD3-associated antigen-binding receptors on suppressor T cells.

Kuchroo, Vijay K.; Steele, J.; Billings, Paul R.; Selvaraj, Periasamy; Dorf, Martin E.

doi:10.1073/pnas.85.23.9209

Cited by 13 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Utilizing the strategies of TcR a or p expression loss variants followed by reconstitution with homologous TcR a cDNA, a critical role for TcR a chain in antigen-specific Ts function was directly demonstrated. (11). This observation has been confirmed in other suppressor T-cell systems (12,24,25).…”

Section: Discussionsupporting

confidence: 72%

“…Initially, donorderived TcR (3-chain rearrangements were not detected in antigen-specific suppressor T-cell (Ts) hybridomas (1,2). Subsequent analyses of Ts hybridomas indicated that Ts do express TcR a-chain mRNA derived from the Ts donor (3,4) and that some express the CD3-TcR a/(3 complex on their surface (5)(6)(7)(8)(9)(10)(11). Selection of Ts hybridomas for expression of surface CD3 resulted in an increase in antigen binding (11) and suppressive bioactivity (11,12).…”

mentioning

confidence: 99%

“…Subsequent analyses of Ts hybridomas indicated that Ts do express TcR a-chain mRNA derived from the Ts donor (3,4) and that some express the CD3-TcR a/(3 complex on their surface (5)(6)(7)(8)(9)(10)(11). Selection of Ts hybridomas for expression of surface CD3 resulted in an increase in antigen binding (11) and suppressive bioactivity (11,12). Molecular analysis of three 4-hydroxy-3-nitrophenyl acetyl (NP)-specific suppressor effector (Ts3) hybridomas indicated that each expressed a donor-derived TcR a chain and a TcR (3 chain derived from the BW5147 fusion partner (4).…”

mentioning

confidence: 99%

See 2 more Smart Citations

T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

Kuchroo

Byrne

Atsumi

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 72%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

Kuchroo

Byrne

Atsumi

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This indicates that the earlier results on the absence of TCR in regulatory T-cell hybridomas must be reevaluated. More recently, Kuchroo et al (25) confirmed and extended these findings by using several Ts3 hybridomas. Selection for CD3' cells resulted in enhanced factor production and yielded populations capable of binding antigen.…”

Section: Discussionsupporting

confidence: 67%

Specific inhibition of cell-surface T-cell receptor expression by antisense oligodeoxynucleotides and its effect on the production of an antigen-specific regulatory T-cell factor.

Zheng

Sahai

Kilgannon

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

We have used antisense oligodeoxynucleotides corresponding to genes encoding the variable (V) region of the T-cell receptor (TCR) a and j3 chains (V. and Vp) to control TCR expression in T-cell hybridomas. Two hybridomas, A1.1 and B1.1, recognize a synthetic polypeptide antigen designated poly 18 {poly[Glu-Tyr-Lys-(Glu-Tyr-Ala)51} together with I-Ad. We have found that TCR function (production of lymphokines in response to antigen) and T3 expression were removed after protease treatment of the cells and were fully recovered 48 hr later. However, when antisense oligodeoxynucleotides corresponding to the appropriate TCR V genes were present after protease treatment, little or no recovery of TCR function or T3 expression was observed. This effect was specific for the TCR V genes utilized by the T cell: antisense oligodeoxynucleotides corresponding to the TCR V regions of AM.1 had no effect on TCR expression in B1.1 and vice versa. Thus, antisense oligodeoxynucleotides can be used to temporarily block expression of a TCR gene in a T-cell hybridoma. This technique was then applied to a paradoxical phenomenon in Al.1 cells. We had observed previously that Al.1 releases an antigen-specific immunoregulatory activity that shows the same antigenic fine specificity as is displayed by the TCR of AL.L. We now report that antisense oligodeoxynucleotides corresponding to the AM.1 V. gene blocked the production of this soluble antigen-specific activity by the cell. Antisense oligodeoxynucleotides corresponding to All Vp, on the other hand, had no effect on the production of this antigen-specific activity. We discuss these observations in the context of recent findings on the nature of T cell-derived antigen-specific regulatory factors.T lymphocytes specifically recognize foreign antigen together with self major histocompatibility complex (MHC) molecules through the cell-surface T-cell receptor (TCR) complex. This complex is composed of the TCR a and p chains, which are responsible for antigen and MHC specificity (1), and the T3 molecules, which may be responsible for transducing the membrane signal (2). a Chain, p chain, and T3 are only expressed as a complex; in the absence ofany one component, cell-surface expression does not occur (2).A helper T-cell hybridoma (A1.1) has been described (3) that expresses TCR a and 8 molecules specific for a synthetic polypeptide designated poly 18 {poly[Glu-Tyr-Lys-(Glu-TyrAla)5]} and, in the presence of specific antigen and I-Ad, releases lymphokines. This T-cell hybridoma also constitutively produces a poly 18-specific cell-free factor involved in antigen-specific induction of suppression, which we call "suppressor-inducer factor" (4). Analysis has revealed (4) that the factor produced by Al. 1 displayed the same antigenic fine specificity exhibited by the TCR on the Al.1 cell surface.Further, an anti-TCR antiserum was found to bind the antigen-specific factor (5). These results led us to speculate that at least some of the genes encoding the TCR a and 83 chains may be responsibl...

show abstract

“…In order to assess the blocking effect of TNP-lysine on specif ic Ag binding by immune LN cells, we utilized a rosette as say [15,16]. TNCB-immune LN cells were incubated for 1 h at 4°C with different concentrations of TNP-lysine in Hank's balanced salt solution (HBSS) (Nissui Pharmaceu tical Co.).…”

Section: Binding Ability O F Tnp-lysine To T-cell Receptorsmentioning

confidence: 99%

Down-Regulation of Murine Contact Sensitivity by Hapten-Amino Acid Derivatives

Satoh¹,

Yokozeki²,

Katayama³

et al. 1993

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The low responsiveness of contact sensitivity (CS) in sensitized and challenged mice was induced by using hapten-amino acid derivatives. Subcutaneous injection of trinitrophenyl (TNP)-lysine or oxazolone-glycine inhibited ear swelling responses in mice that received rechallenge. The inhibitory effect was long-lasting and antigen-specific. TNP-lysine blocked the transfer of CS in vivo and the binding of immune lymph node cells to antigen in vitro. Thus, the suppression was mediated by inhibition of effector cells, but not associated with the generation of efferent limb-acting suppressor cells. The tool reported herein seems to be quite useful for controlling ongoing murine CS to simple chemicals.

show abstract

Expression of CD3-associated antigen-binding receptors on suppressor T cells.

Cited by 13 publications

References 34 publications

T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

Specific inhibition of cell-surface T-cell receptor expression by antisense oligodeoxynucleotides and its effect on the production of an antigen-specific regulatory T-cell factor.

Down-Regulation of Murine Contact Sensitivity by Hapten-Amino Acid Derivatives

Contact Info

Product

Resources

About