Expression of CD4⁺ forkhead box P3 (FOXP3)⁺ regulatory T cells in inflammatory bowel disease

Abstract

METHODS: The percentage of CD4+ FOXP3 + Treg cells in PBMC was analyzed by flow cytometry. Immunohistochemistry was used to examine the FOXP3 + cells in the inflamed mucosa. Real-time polymerase chain reaction and Western blot were used to detect the expressions of FOXP3 mRNA and protein in PBMC and mucosal biopsy specimens of IBD patients, respectively.

Interleukin (IL)-23 Suppresses IL-10 in Inflammatory Bowel Disease

“…Diverse data about Tregs in IBD have been reported. Wang et al (36) recently indicated that the frequency of Tregs was increased in the peripheral blood and the IBD intestinal mucosa. This phenomenon implies that Treg function is compromised in IBD patients.…”

Hepatocellular Carcinoma Cells Induce Regulatory T Cells and Lead to Poor Prognosis via Production of Transforming Growth Factor-β1

“…Total RNA was extracted from the cultured cells as described previously [25]. The relative expression levels of TGF-β1 and Foxp3 mRNA were detected by real-time PCR (ABI7500 Fast system, UAS) using a SYBR Prime Script kit (TaKaRa, Dalian, China).…”

“…Immunohistochemical analysis was performed using mouse anti-human TGF-β1 primary antibody (Santa Cruz, Texas, USA), mouse anti-human Foxp3 primary antibody (236A/E7, Abcam), and rabbit anti- mouse Foxp3 primary antibody (Abcam), as described previously [25]. The cut-off value for Treg expression was the median value.…”

“…Human Tregs were detected using a Treg-staining kit (eBioscience, California, USA), as described previously [25]. Mouse Tregs were detected using peridinin chlorophyll protein complex-conjugated antimouse CD4 antibody (BD Bioscience, California, USA) and phycoerythrin-conjugated anti-mouse Foxp3 Ab (BD).…”

Exploring the role of the microbiota member Bifidobacterium in modulating immune-linked diseases

“…Exacerbated Th1 or Th17 responses have been linked to auto-immune disease [46], whereas uncontrolled Th2 responses or reduced Treg responses are associated with allergic reactions [47]. A lack of Tregs is often also found in patients with IBD [48]. Notably, several studies have reported that different strains of bifidobacteria can modulate T-cell responses in immune-driven diseases.…”