Expression of connexins in human preimplantation embryos in vitro

Bloor, Debra; Wilson, Yvonne; Kibschull, Mark; Traub, Otto; Leese, Henry J.; Winterhager, Elke; Kimber, Susan J.

doi:10.1186/1477-7827-2-25

Cited by 38 publications

(7 citation statements)

References 36 publications

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“…Gerbaud et al found that Cx43 accelerated trophoblast fusion process by activating protein kinase A (PKA)-dependent phosphorylation [ 35 ]. Cx43 is an important component for gap junction formation in human preimplantation embryos [ 36 ]. Rmanathal et al found that Cx43 played critical role in controlling the paracrine secretions of stromal cells under decidualization, which may alter the proliferation and migration of endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

Chen

2016

Reprod Biol Endocrinol

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BackgroundApproximately 45–50 % of the recurrent pregnancy loss (RPL) remain(s) unexplained that challenges its clinical management. Formation and development of placenta as well as angiogenesis are critical for successful pregnancy. Vascular endothelial growth factor (VEGF) and connexin 43 (Cx43) play important roles in angiogenesis and placenta development and aberration of these have been linked to RPL. We aimed to investigate whether the expressions of VEGF and Cx43 were altered in the first-trimester tissues (chorionic villi and decidua) collected from women with RPL compared to those from healthy early pregnant women.MethodsFirst-trimester chorionic villi and decidua were collected from pregnant women diagnosed RPL who ended up with surgical intervention (n = 28) in comparison to those collected from women requesting surgical termination of their unwanted normal first-trimester pregnancies (n = 28). These two groups of women were matched in age and gestational weeks. Tissues were analyzed for the protein and messenger ribonucleic acid (mRNA) expressions of Cx43 and VEGF by immunohistochemistry, western blot, and quantitative reverse transcription polymerase chain reaction (qRT-PCR).ResultsThe expressions of both Cx43 and VEGF at the level of mRNA and protein in the villi and decidua from women with RPL were significantly decreased compared with those from women with normal early pregnancy.ConclusionsReduction of Cx43 and VEGF expressed in the first-trimester tissues might indicate their important roles involved in RPL and thus holds the potential to develop pharmaceutical therapies for treatment of RPL.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-016-0179-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

Chen

2016

Reprod Biol Endocrinol

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“…Xenopus Cx31 and Cx43.4, the orthologues of hCx31 and Cx45, are expressed in both oocytes and early embryos (41). Cx31 and Cx45 transcripts occur in the pronucleate to four cell stage during human embryogenesis (42). Thus, heterotypic coupling between Cx31 and Cx45 may be important in early stages of development.…”

Section: Discussionmentioning

confidence: 99%

Properties of human connexin 31, which is implicated in hereditary dermatological disease and deafness

Abrams

Freidin²,

Verselis³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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The connexins are a family of at least 20 homologous proteins in humans that form aqueous channels connecting the interiors of coupled cells and mediating electrical and chemical communication. Mutations in the gene for human connexin 31 (hCx31) are associated with disorders of the skin and auditory system. Alterations in functional properties of Cx31 junctions are likely to play a role in these diseases; nonetheless, little is known about the properties of the wild-type channels. Here we show that hCx31 channels, like other connexin channels, are gated by voltage and close at low pH and when exposed to long-chain alkanols. Singlechannel conductance of the fully open channel is Ϸ85 pS, and it is permeable to Lucifer yellow, Alexa Fluor 350 , ethidium bromide, and DAPI, which have valences of ؊2, ؊1, ؉1, and ؉2, respectively. In contrast to what has been reported for mouse Cx31, hCx31 appears to form functional heterotypic channels with all four connexins tested, Cx26, Cx30, Cx32, and Cx45. These findings provide an important first step in evaluating the pathogenesis of inherited human diseases associated with mutations in the gene for Cx31.electrical coupling ͉ erythrokeratodermia variabilis ͉ gap junction ͉ intercellular communication

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“…Cx43 is one of the most important proteins present in the gap junction channels located between mammalian ventricular muscle cells ( 49 ). It serves an important role in cardiac development, normal cardiac electrical activity, heart diastolic movement and the differentiation of stem cells to myocardial cells ( 50 ).…”

Section: Discussionmentioning

confidence: 99%

A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells

Lao

Liu

et al. 2017

Molecular Medicine Reports

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In the present study, subcutaneous fat was obtained from adult women that had undergone conventional liposuction surgery. A comparative study was performed to investigate the effect of transparent and white poly-β-hydroxyethyl methacrylate (PHEMA) stents, which have different surface and cross-sectional morphological characteristics, on the differentiation of adipose-derived stem cells (ASCs) into myocardial cells. The cell counting kit-8 assay revealed that cell growth increased at varying rates among the different treatment groups. The absorbance of the experimental transparent PHEMA treated group increased in a time-dependent manner with the duration of incubation. The highest levels of proliferation were observed in the transparent PHEMA group. In addition, the transparent PHEMA treated group exhibited the strongest cell adhesion ability, which was significantly different to that of the white PHEMA group (P<0.01 and P<0.05 for Matrigel and fibronectin assay, respectively). Comparisons between the two stent materials with the inducer control group revealed statistically significant differences in the rate of ASC differentiation (P<0.05). The level of differentiation was the greatest in the transparent PHEMA group, and was significantly different to the white PHEMA group (P<0.05) and the blank control group (P<0.01). The results suggest that the inducers 5-aza-2-deoxycytidin and laminin, and material microstructure stents effectively promote the proliferation, growth and adhesion of ASCs. However, the transparent material microstructure may be a more suitable candidate for ASC-associated injections. The present study provides further evidence that a PHEMA stent structure, comprised of a high number of matrixes and a low water content, induces a high level of ASC differentiation to myocardial cells.

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Expression of connexins in human preimplantation embryos in vitro

Cited by 38 publications

References 36 publications

Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

Reduced expressions of connexin 43 and VEGF in the first-trimester tissues from women with recurrent pregnancy loss

Properties of human connexin 31, which is implicated in hereditary dermatological disease and deafness

A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells

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