Expression of CPEB4 in Human Glioma and Its Correlations With Prognosis

Abstract

CPEB4 plays an important role in cancer progression. However, the clinicopathological significance of CPEB4 expression to glioma and its expression levels in glioma tissues and cell lines are unknown. The present study investigated the potential prognostic value of CPEB4 for human glioma.Immunohistochemistry (IHC) was performed to examine the dynamics of CPEB4 expression in glioma and nonneoplastic brain tissues, and the expression of CPEB4 in cell lines and freshly prepared tissue samples was measured using Western blotting and real-time PCR.CPEB4 was highly expressed at the mRNA and protein levels in 4 glioma cell lines and in 4 freshly prepared glioma tissues. Immunohistochemical analysis demonstrated that CPEB4 expression in glioma tissue was higher than that in corresponding nonneoplastic brain tissue (P < 0.01). This high expression level was further increased in high-grade gliomas, and the CPEB4 expression level correlated with the WHO classification (r = 0.774, P < 0.01). Moreover, the overall survival of glioma patients displaying high CPEB4 protein expression (P < 0.01) was clearly lower than that of those displaying low CPEB4 expression, and the high CPEB4 expression indicated a poorer survival in high-grade glioma patients (P < 0.01).Our study suggests that CPEB4 is significantly expressed in human glioma and that the upregulation of CPEB4 protein is significantly associated with advanced WHO grade. CPEB4 may serve as a highly sensitive prognostic indicator for glioma patients.

Downregulated cytoplasmic polyadenylation element-binding protein-4 is associated with the carcinogenesis of head and neck squamous cell carcinoma

“…The association between CPEB4 and histological grading has been identified in several previous studies, indicating that CPEB4 expression was positively associated with differing tumor grades (22,32,33). In the present study, the analysis of the GEO datasets suggested that downregulated CPEB4 expression further decreased in association with increasing histological grades of HNSCC.…”

“…In the present study, CPEB4 expression was investigated between various types of cancer and corresponding normal tissues. Notably in previous studies, the expression of CPEB4 protein was increased in several malignant tumors, including metastatic prostate cancer (34), colorectal cancer (CRC) (31), astrocytic tumors (32), invasive ductal breast carcinoma (IDC) (22) and glioma (33). In contrast, another study reported the opposite outcome in that CPEB4 protein was downregulated in hepatocellular carcinoma and non-small cell lung cancer (35).…”

“…Ortiz-Zapater et al (21) initially demonstrated the direct link between CPEB4 expression and cancer etiology, and suggested that overexpression may be a common mechanism for regulating the reprogramming of gene expression involved in cancer progression. Subsequently, a number of studies have been performed that demonstrated that the aberrant expression of CPEB4 is significantly associated with the clinical prognosis of patients with malignant tumors (22,(31)(32)(33).…”

MicroRNA-203-mediated posttranscriptional deregulation of CPEB4 contributes to colorectal cancer progression

“…Recent studies have demonstrated that CPEB4 is highly expressed in several malignant tumors [8,9], but little is known about its expression and functional role in colorectal cancer. In this study, we explored CPEB4 expression levels in clinical colorectal cancer specimens and revealed that CPEB4 was aberrant expressed in colorectal cancer tissues and negatively correlated with survival, which is consistent with previous studies [10].…”

“…CPEB4, a typical member of the CPEB family, can modulate the cellular epigenetic profile and impact several biological activities [5e7]. Recently, aberrant high expression level of CPEB4 has been demonstrated in a variety of malignancies, including pancreatic ductal adenocarcinomas, metastatic prostate cancer and glioma compared with their corresponding normal tissues [6,8,9]. Importantly, it has been reported that CPEB4 plays oncogenic role in pancreatic ductal adenocarcinoma by promoting cell proliferation and invasion, and could be exploited as a target for cancer treatment [6].…”