Expression of CT-wpr, screened by cDNA-AFLP approach, associated with hydroxysafflor yellow A in Carthamus tinctorius L.

Li, Yakui; Zhang-jian, Wang; Chang, Hong; Wang, Yan; Guo, Meili

doi:10.1016/j.bse.2010.10.010

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…sHSP is a small heat shock protein and encoded by CTL- hsyapr, which might be directly or indirectly disturb HSYA biosynthetic pathway ( Tang et al, 2010 ). When safflower is under external pressure, CT- wpr (TDF-11) was activated, which might arouse sHSP and inhibit the expression of HSya to some extent, finally leading to the inhibition of biosynthetic pathway of HSYA ( Li et al, 2010b ).…”

Section: Acquisition Of Hydroxysafflower Yellow Amentioning

confidence: 99%

Hydroxysafflor Yellow A: A Systematical Review on Botanical Resources, Physicochemical Properties, Drug Delivery System, Pharmacokinetics, and Pharmacological Effects

et al. 2020

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Hydroxysafflower yellow A (HSYA), as a principal natural ingredient extracted from safflower (Carthamus tinctorius L.), has significant pharmacological activities, such as antioxidant, anti-inflammatory, anticoagulant, and anticancer effects. However, chemical instability and low bioavailability have been severely hampering the clinical applications of HSYA during the treatment of cardiovascular and cerebrovascular disease. Therefore, this present review systematically summarized the materials about HSYA, including acquisition methods, extraction and detection methods, pharmacokinetics, pharmacological effects and molecular mechanism, especially focus on the possible causes and resolutions about the chemical instability and low bioavailability of HSYA, in order to provide relatively comprehensive basic data for the related research of HSYA.

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Section: Acquisition Of Hydroxysafflower Yellow Amentioning

confidence: 99%

Hydroxysafflor Yellow A: A Systematical Review on Botanical Resources, Physicochemical Properties, Drug Delivery System, Pharmacokinetics, and Pharmacological Effects

et al. 2020

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“…Multiple enzymes (such as UGTs) and TFs (such as bHLHs and MYBs) coexpressed with CarCHS s would contribute to the complexity of flavonoid biosynthesis in safflower (Figure S49 ). For example, the antisense RNA ( CT‐wpr ) of the coexpressed CtAH10G0189700 (encoding a member of the aspartyl protease family, ASP) was significantly associated with the presence of HSYA and was up‐regulated in flowers lacking HSYA (Li et al., 2010 ). By contrast, CtAH10G0189700 was up‐regulated in flowers containing HSYA compared with flowers lacking HSYA.…”

Section: Discussionmentioning

confidence: 99%

“…Natural antisense transcripts are endogenous RNA molecules containing sequences that are complementary to other transcripts and can negatively regulate the corresponding sense transcript (Lapidot and Pilpel, 2006 ). Therefore, the sense transcript ( CtAH10G0189700 ) was possibly regulated by its antisense transcript ( CT‐wpr ), which likely functions as a noncoding RNA to suppress the HSYA biosynthetic pathway (Li et al., 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…For years, efforts have been made to understand the molecular mechanisms underlying the two most important traits in safflower, fatty acid composition (Golkar et al., 2011 ) and flavonoid biosynthesis, via genetic analyses (Li et al., 2010 ). Many flavonoid biosynthesis genes have been cloned in safflower, including those encoding chalcone synthases (CHSs), UDP‐glucuronosyltransferases (UGTs), chalcone isomerases (CHIs) and flavanone 3‐hydroxylases (F3Hs; Chen et al., 2020 ); however, the evolution and regulation of gene families involved the biosynthesis of flavonoids such as HSYA remain unclear at the genomic level.…”

Section: Introductionmentioning

confidence: 99%

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The chromosome‐scale reference genome of safflower (Carthamus tinctorius) provides insights into linoleic acid and flavonoid biosynthesis

Liu

Zhan

et al. 2021

Plant Biotechnology Journal

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Summary Safflower (Carthamus tinctorius L.), a member of the Asteraceae, is a popular crop due to its high linoleic acid (LA) and flavonoid (such as hydroxysafflor yellow A) contents. Here, we report the first high‐quality genome assembly (contig N50 of 21.23 Mb) for the 12 pseudochromosomes of safflower using single‐molecule real‐time sequencing, Hi‐C mapping technologies and a genetic linkage map. Phyloge nomic analysis showed that safflower diverged from artichoke (Cynara cardunculus) and sunflower (Helianthus annuus) approximately 30.7 and 60.5 million years ago, respectively. Comparative genomic analyses revealed that uniquely expanded gene families in safflower were enriched for those predicted to be involved in lipid metabolism and transport and abscisic acid signalling. Notably, the fatty acid desaturase 2 (FAD2) and chalcone synthase (CHS) families, which function in the LA and flavonoid biosynthesis pathways, respectively, were expanded via tandem duplications in safflower. CarFAD2‐12 was specifically expressed in seeds and was vital for high‐LA content in seeds, while tandemly duplicated CarFAD2 genes were up‐regulated in ovaries compared to CarFAD2‐12, which indicates regulatory divergence of FAD2 in seeds and ovaries. CarCHS1, CarCHS4 and tandem‐duplicated CarCHS5˜CarCHS6, which were up‐regulated compared to other CarCHS members at early stages, contribute to the accumulation of major flavonoids in flowers. In addition, our data reveal multiple alternative splicing events in gene families related to fatty acid and flavonoid biosynthesis. Together, these results provide a high‐quality reference genome and evolutionary insights into the molecular basis of fatty acid and flavonoid biosynthesis in safflower.

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“…(1) Differentiate homologous gene copies which is an advantage over microarray (2) The specificity and sensitivity of this technique detects poorly expressed genes as well as distinguishes between homologous sequences Amaranthus caudatus SSH Calcium stress-induced genes [111] Camellia sinensis cDNA microarray Analysis of albescent stages from 'Anji Baicha [253] Camellia sinensis Microarray Large-scale gene expression [174] Camellia sinensis SSH Formation of adventitious root induced by indole-3-butyric acid [254] Carthamus tinctorius cDNA-AFLP Effect of CT-wpr expression on hydroxysafflor yellow A [255] Carthamus tinctorius cDNA-AFLP Transcripts related to biosynthetic of hydroxysafflor yellow A [256] Carthamus tinctorius cDNA-AFLP Expression of CTL-hsyapr in associate with hydroxysafflor yellow A [257] Catharanthus roseus cDNA-AFLP Responsive genes against wheat blue dwarf phytoplasma [258] Chrysanthemum lavandulifolium cDNA-AFLP salt-inducible genes [259] Citrus aurantifolia SSH Resistance responsive genes against citrus tristeza virus [104] Citrus reticulata Blanco Microarray Transcriptome analysis of a citrus late-ripening [260] Cucumis sativus SSH Powdery mildew-resistant genes [105] Disambiguation SSH Ethylene climacteric-induced genes [112] Eustoma grandiflorum Microarray Floral transcriptome [261] Festuca arundinacea Microarray Responsive genes involved in endophyte infection [262] Fragaria chiloensis SSH Responsive genes in development and ripening of fruit [99] Glycine max SSH Salt stress-induced genes [101] Gossypium arboreum SSH Resistance responsive genes against Apolygus lucorum [106] Gossypium hirsutum cDNA-AFLP Responsive genes against infection of Verticillium dahliae [263] Gossypium hirsutum Microarray Salt-responsive genes [264] Helianthus annuus SSH Phosphorus stress-induced genes [113,115] Lepidium sativum SSH Peroxidases [115] Melon Fruit SSH Expressed genes during ethylene climacteric [112] Musa acuminate SSH Tolerance to Fusarium oxysporum [107] Nicotiana tabacum cDNA-AFLP Responsive genes against tobacco mosaic virus …”

Section: Sshmentioning

confidence: 99%

Suppression Subtractive Hybridization Versus Next-Generation Sequencing in Plant Genetic Engineering: Challenges and Perspectives

et al. 2015

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Suppression subtractive hybridization (SSH) is an effective method to identify different genes with different expression levels involved in a variety of biological processes. This method has often been used to study molecular mechanisms of plants in complex relationships with different pathogens and a variety of biotic stresses. Compared to other techniques used in gene expression profiling, SSH needs relatively smaller amounts of the initial materials, with lower costs, and fewer false positives present within the results. Extraction of total RNA from plant species rich in phenolic compounds, carbohydrates, and polysaccharides that easily bind to nucleic acids through cellular mechanisms is difficult and needs to be considered. Remarkable advancement has been achieved in the next-generation sequencing (NGS) field. As a result of progress within fields related to molecular chemistry and biology as well as specialized engineering, parallelization in the sequencing reaction has exceptionally enhanced the overall read number of generated sequences per run. Currently available sequencing platforms support an earlier unparalleled view directly into complex mixes associated with RNA in addition to DNA samples. NGS technology has demonstrated the ability to sequence DNA with remarkable swiftness, therefore allowing previously unthinkable scientific accomplishments along with novel biological purposes. However, the massive amounts of data generated by NGS impose a substantial challenge with regard to data safe-keeping and analysis. This review examines some simple but vital points involved in preparing the initial material for SSH and introduces this method as well as its associated applications to detect different novel genes from different plant species. This review evaluates general concepts, basic applications, plus the probable results of NGS technology in genomics, with unique mention of feasible potential tools as well as bioinformatics.

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Expression of CT-wpr, screened by cDNA-AFLP approach, associated with hydroxysafflor yellow A in Carthamus tinctorius L.

Cited by 8 publications

References 23 publications

Hydroxysafflor Yellow A: A Systematical Review on Botanical Resources, Physicochemical Properties, Drug Delivery System, Pharmacokinetics, and Pharmacological Effects

Hydroxysafflor Yellow A: A Systematical Review on Botanical Resources, Physicochemical Properties, Drug Delivery System, Pharmacokinetics, and Pharmacological Effects

The chromosome‐scale reference genome of safflower (Carthamus tinctorius) provides insights into linoleic acid and flavonoid biosynthesis

Suppression Subtractive Hybridization Versus Next-Generation Sequencing in Plant Genetic Engineering: Challenges and Perspectives

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