Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli

Gao, Guanjun; Teng, Bing; Liu, Li‐Li; Sheng, Duohong; Shen, Binghui; Hua, Yuejin

doi:10.1016/j.dnarep.2003.08.012

Cited by 58 publications

(48 citation statements)

References 31 publications

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“…75, 2011 D. RADIODURANS OXIDATIVE STRESS RESISTANCE 165 (203) and may regulate the transcription of genes through a more classic transcription factor (642). The expression of irrE in E. coli slightly enhances the expression of RecA (1.5-fold), radiation resistance (1.6-fold), the scavenging of O 2 ⅐ Ϫ and H 2 O 2 , and both the constitutive (2.4-fold) and radiation-induced (4.3-fold) activities of KatG (203). Furthermore, the heterologous expression of irrE increases stress tolerance and ethanol production in E. coli; in a …”

Section: Stress Response Transcriptional Regulatorsmentioning

confidence: 99%

Oxidative Stress Resistance inDeinococcus radiodurans

Slade

Radman

2011

Microbiol Mol Biol Rev

660

639

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SUMMARY Deinococcus radiodurans is a robust bacterium best known for its capacity to repair massive DNA damage efficiently and accurately. It is extremely resistant to many DNA-damaging agents, including ionizing radiation and UV radiation (100 to 295 nm), desiccation, and mitomycin C, which induce oxidative damage not only to DNA but also to all cellular macromolecules via the production of reactive oxygen species. The extreme resilience of D. radiodurans to oxidative stress is imparted synergistically by an efficient protection of proteins against oxidative stress and an efficient DNA repair mechanism, enhanced by functional redundancies in both systems. D. radiodurans assets for the prevention of and recovery from oxidative stress are extensively reviewed here. Radiation- and desiccation-resistant bacteria such as D. radiodurans have substantially lower protein oxidation levels than do sensitive bacteria but have similar yields of DNA double-strand breaks. These findings challenge the concept of DNA as the primary target of radiation toxicity while advancing protein damage, and the protection of proteins against oxidative damage, as a new paradigm of radiation toxicity and survival. The protection of DNA repair and other proteins against oxidative damage is imparted by enzymatic and nonenzymatic antioxidant defense systems dominated by divalent manganese complexes. Given that oxidative stress caused by the accumulation of reactive oxygen species is associated with aging and cancer, a comprehensive outlook on D. radiodurans strategies of combating oxidative stress may open new avenues for antiaging and anticancer treatments. The study of the antioxidation protection in D. radiodurans is therefore of considerable potential interest for medicine and public health.

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Section: Stress Response Transcriptional Regulatorsmentioning

confidence: 99%

Oxidative Stress Resistance inDeinococcus radiodurans

Slade

Radman

2011

Microbiol Mol Biol Rev

660

639

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“…Expression of the novel gene, driven by the promoter of D. radiodurans groEL (DR0607), also significantly enhanced the resistance of E. coli to ␥ irradiation. We found that the expression of PprI in E. coli also increased the expression of RecA and improved catalase activity (24). Both microarray and Western blotting analysis demonstrated that ionizing radiation did not increase PprI transcription or trans-lation levels (19,(25)(26)(27).…”

mentioning

confidence: 99%

Deinococcus radiodurans PprI Switches on DNA Damage Response and Cellular Survival Networks after Radiation Damage

Gao

et al. 2009

Molecular & Cellular Proteomics

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Preliminary findings indicate that PprI is a regulatory protein that stimulates transcription and translation of recA and other DNA repair genes in response to DNA damage in the extremely radioresistant bacterium Deinococcus radiodurans. To define the repertoire of proteins regulated by PprI and investigate the in vivo regulatory mechanism of PprI in response to ␥ radiation, we performed comparative proteomics analyses on wild type (R1) and a pprI knock-out strain ( The Gram-positive nonpathogenic bacterium Deinococcus radiodurans is characterized by extreme resistance to ionizing radiation, UV irradiation, desiccation, and a variety of DNAdamaging agents without resulting in lethality or mutagenesis (1, 2). This dramatic capability is ascribed to its outstanding efficiency in reconstructing a functional genome with high fidelity from hundreds of double strand breaks (DSBs) 1 generated by DNA-damaging agents (2, 3), whereas few other organisms can tolerate DSBs (4). Exponentially growing D. radiodurans is able to withstand 50 -100 times more ionizing radiation than Escherichia coli and can survive a 15-kGy acute ionizing radiation dose with no loss of viability. Its ability of continuous growth without any delay when exposed to a maximum of 60 Gy/h ␥ ray (5) has made it one of the most distinguished candidates for bioremediation of radioactive wastes and contaminants (6, 7). More than 50 years of research has provided many lines of evidence supporting the benefits of the extreme radioresistance of D. radiodurans from its highly efficient DNA damage repair system and its remarkable antioxidation system (1, 8 -15). However, the mechanism underlying its radioresistance is still not completely understood (4, 9). Intriguingly this bacterium not only possesses most of the DNA repair genes found in other organisms but also contains many proteins of yet to be determined functions that have been revealed by genome sequencing and comparative genomics (16,17). These function-unknown proteins may play crucial roles in radioresistance (9, 18) as implied by several studies in this bacterium (19 -23).Several years ago, our group and that of John Battista (19, 22) identified and validated a novel protein, PprI (also named IrrE), essential in D. radiodurans radioresistance. It strongly enhanced catalase activities and promoted the expression of RecA and PprA (19). Expression of the novel gene, driven by the promoter of D. radiodurans groEL (DR0607), also significantly enhanced the resistance of E. coli to ␥ irradiation. We found that the expression of PprI in E. coli also increased the expression of RecA and improved catalase activity (24). Both microarray and Western blotting analysis demonstrated that ionizing radiation did not increase PprI transcription or trans-

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“…This approach has been successful in other contexts: for instance, E. coli's resistance to gamma irradiation, to desiccation and to low temperatures has been increased by expressing, respectively, the PprI protein from Deinococcus radiodurans (Gao et al 2003), a sucrose-6-phosphate synthase from Synechocystis sp. strain PCC 6803 (Billi et al 2000b) and the chaperonin Cpn60 and cochaperonin Cpn10 from the psychrophilic bacterium Oleispira antarctica (Ferrer et al 2003).…”

Section: Engineering Cyanobacteriamentioning

confidence: 99%

Sustainable life support on Mars – the potential roles of cyanobacteria

Verseux

Baqué

Lehto

et al. 2015

International Journal of Astrobiology

144

136

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Even though technological advances could allow humans to reach Mars in the coming decades, launch costs prohibit the establishment of permanent manned outposts for which most consumables would be sent from Earth. This issue can be addressed by in situ resource utilization: producing part or all of these consumables on Mars, from local resources. Biological components are needed, among other reasons because various resources could be efficiently produced only by the use of biological systems. But most plants and microorganisms are unable to exploit Martian resources, and sending substrates from Earth to support their metabolism would strongly limit the cost-effectiveness and sustainability of their cultivation. However, resources needed to grow specific cyanobacteria are available on Mars due to their photosynthetic abilities, nitrogen-fixing activities and lithotrophic lifestyles. They could be used directly for various applications, including the production of food, fuel and oxygen, but also indirectly: products from their culture could support the growth of other organisms, opening the way to a wide range of life-support biological processes based on Martian resources. Here we give insights into how and why cyanobacteria could play a role in the development of self-sustainable manned outposts on Mars.

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Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli

Cited by 58 publications

References 31 publications

Oxidative Stress Resistance inDeinococcus radiodurans

Oxidative Stress Resistance inDeinococcus radiodurans

Deinococcus radiodurans PprI Switches on DNA Damage Response and Cellular Survival Networks after Radiation Damage

Sustainable life support on Mars – the potential roles of cyanobacteria

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