Expression of epidermal growth factor receptor and vascular endothelial growth factor in malignant canine epithelial nasal tumours*

Shiomitsu, Keijiro; Johnson, C. L.; Malarkey, David E.; Pruitt, Amy F.; Thrall, Donald E.

doi:10.1111/j.1476-5829.2008.00178.x

Cited by 35 publications

(44 citation statements)

References 39 publications

(52 reference statements)

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“…As a result of the research, evaluation of the expression of VEGF and its receptors was demonstrated to be a reliable prognostic factor in squamous cell carcinoma of the oral cavity and tongue in humans, as well as in those of the oral cavity and nasal epithelium in dogs. Owing to this, the development and local recurrence as well as the formation of distant metastases can be monitored (Smith et al, 2000;López de Cicco et al, 2004;Shiomitsu et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

Elevated Vascular Endothelial Growth Factor (VEGF) levels in the blood serum of dogs with malignant neoplasms of the oral cavity

Sobczyńska-Rak

Polkowska

Silmanowicz

2014

Acta Veterinaria Hungarica

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Angiogenesis plays an essential role in the development of a neoplastic tumour by conditioning both its growth and the formation of metastases. The induction of blood vessel growth occurs under the influence of proangiogenic factors, among which Vascular Endothelial Growth Factor (VEGF) seems to be the most important. The aim of this research was to study the level of VEGF measured by ELISA in the serum of dogs with neoplasms of the oral cavity. The study material comprised samples of neoplastic tissue from 17 operated dogs and the serum of the examined animals as well as of dogs from the control group. The tissue samples were taken from dogs of different breeds, aged 6–14 years. The tumour type was determined in accordance with the applicable WHO classification. Blood samples taken from sick dogs and from animals of the control group were centrifuged, and immunoenzymatic labelling of VEGF was performed in the obtained serum using ELISA and R&D system reagents (Quantikine Canine VEGF). All stages of VEGF labelling were performed according to the recommendation of the test manufacturer. The median of VEGF in the serum of the dogs with neoplasms of the oral cavity was 40.64 pg/mL. The lowest value of 14.26 pg/mL was observed in the case of fibrosarcoma, and the highest value of 99.19 pg/mL in the case of squamous cell carcinoma. The VEGF median in the control group amounted to 11.14 pg/mL whereas the VEGF value in the groups of animals diagnosed with benign tumours ranged between 2.30 and 19.74 pg/mL. Elevated VEGF in the blood serum, in comparison with the benign tumour group and the control group, was observed in all examined neoplasms of the oral cavity. It was suggested that overexpression of VEGF can have a prognostic value and is useful in the early detection of neoplasms.

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Section: Discussionmentioning

confidence: 99%

Elevated Vascular Endothelial Growth Factor (VEGF) levels in the blood serum of dogs with malignant neoplasms of the oral cavity

Sobczyńska-Rak

Polkowska

Silmanowicz

2014

Acta Veterinaria Hungarica

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“…Expression of its receptor was observed in most studied cases of squamous skin cancers in cats -it correlated with shorter life expectancy but not with the tumour's mitotic activity (29). Incorrect activation of the EGFR receptor was also identified in epithelial tumours with high metastatic potential located in the lungs, nasal cavity and mamma in dogs (30)(31)(32) and oral cavity in cats (33).…”

Section: Discussionmentioning

confidence: 99%

Elevated EGF Levels in the Blood Serum of Dogs with Periodontal Diseases and Oral Tumours

Sobczyńska-Rak

Żylińska

Polkowska

et al. 2018

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“…Those studies have made it possible to use the expression of VEGF and its receptors as a significant prognostic factor in cases of SCC of the oral cavity in humans as well as SCC of the oral cavity and nasal epithelium in animals. Thus it is possible to monitor the development and local relapse of the disease, as well as the incidence of distant metastases (19).…”

Section: Resultsmentioning

confidence: 99%

Vascular endothelial growth factor (VEGF) expression in dogs suffering from squamous cell carcinoma

Sobczyńska-Rak¹,

Polkowska²,

Gołyńska³

et al. 2017

Medycyna Weterynaryjna

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Sobczyńska-Rak A., Polkowska I., Gołyńska M., Szponder T., Żylińska B., Łopuszyński W.Vascular endothelial growth factor expression in dogs suffering from squamous cell carcinoma SummaryThe aim of the present study was to monitor VEGF expression in the blood serum of dogs suffering from squamous cell carcinoma in correlation with the speed of metastasis. The research material consisted of tumour tissue and blood samples collected from 26 dogs of various breeds, aged between 5 and 15 years, and 10 control group animals. Blood samples were centrifuged, and the serum obtained was examined by immunoenzymatic assays to determine vascular endothelial growth factor (VEGF) levels. The study group animals were observed for a period of 6 months, during which their blood was drawn twice for immunoenzymatic tests. In all study group animals, elevated blood serum VEGF levels were observed on the day of surgery compared with the control group. In 14 cases, disease relapse was observed with metastases to the bones or lungs. In all relapsing animals, significantly higher blood serum VEGF levels were observed.VEGF levels are closely related to the neoplastic process, but the exact mechanism of the factor's involvement in neoplastic progression remains unclear, as does the complexity of the angiogenic processes. Studies of the mechanisms affecting tumour proliferation and the monitoring of VEGF levels can facilitate better diagnostics and prognostics, as well as improve the effectiveness of cancer treatment and the planning of new therapeutic strategies for both human and animal patients.

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Expression of epidermal growth factor receptor and vascular endothelial growth factor in malignant canine epithelial nasal tumours*

Cited by 35 publications

References 39 publications

Elevated Vascular Endothelial Growth Factor (VEGF) levels in the blood serum of dogs with malignant neoplasms of the oral cavity

Elevated Vascular Endothelial Growth Factor (VEGF) levels in the blood serum of dogs with malignant neoplasms of the oral cavity

Elevated EGF Levels in the Blood Serum of Dogs with Periodontal Diseases and Oral Tumours

Vascular endothelial growth factor (VEGF) expression in dogs suffering from squamous cell carcinoma

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